製品: SEPT2 Antibody
カタログ: DF4458
タンパク質の説明: Rabbit polyclonal antibody to SEPT2
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 41 KD; 41kD(Calculated).
ユニプロット: Q15019
RRID: AB_2836813

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
SEPT2 Antibody detects endogenous levels of total SEPT2.
RRID:
AB_2836813
引用形式: Affinity Biosciences Cat# DF4458, RRID:AB_2836813.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

DIFF6; hNEDD5; KIAA0158; NEDD 5; NEDD5; Neural precursor cell expressed developmentally down regulated 5; Neural precursor cell expressed developmentally down regulated protein 5; Pnutl3; Protein NEDD5; SEPT2; septin 2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q15019 SEPT2_HUMAN:

Widely expressed. Up-regulated in liver cancer.

タンパク質配列:
MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVIPGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Zebrafish
92
Chicken
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15019 基板として

Site PTM Type Enzyme
K3 Ubiquitination
T7 Phosphorylation
T14 Phosphorylation
Y17 Phosphorylation
S31 Phosphorylation
K74 Ubiquitination
T78 Phosphorylation
R96 Methylation
C111 S-Nitrosylation
K116 Ubiquitination
S120 Phosphorylation
S134 Phosphorylation
K174 Ubiquitination
K183 Ubiquitination
K190 Acetylation
K190 Ubiquitination
K209 Ubiquitination
Y211 Phosphorylation
S218 Phosphorylation P68400 (CSNK2A1)
K225 Ubiquitination
T228 Phosphorylation
K232 Acetylation
K232 Ubiquitination
K249 Ubiquitination
K275 Ubiquitination
T291 Phosphorylation
Y296 Phosphorylation
K310 Ubiquitination
K318 Ubiquitination
K325 Ubiquitination

研究背景

機能:

Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

細胞の位置付け:

Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Spindle. Chromosome>Centromere>Kinetochore. Cleavage furrow. Midbody. Cytoplasm>Cell cortex. Cell projection>Cilium membrane. Cell projection>Cilium>Flagellum.
Note: In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria. In epithelial cells, colocalizes with polyglutamylated tubulin around the trans-Golgi network, as well as juxatnuclear and proximal Golgi apparatus. Localizes at the base of the cilia near the morphological distinction between the cilia and plasma membranes. Found in the sperm annulus (PubMed:25588830).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Up-regulated in liver cancer.

サブユニット構造:

Septins polymerize into heterooligomeric protein complexes that form filaments, and associate with cellular membranes, actin filaments and microtubules Ref.36). GTPase activity is required for filament formation. Filaments are assembled from asymmetrical heterotrimers, composed of SEPTIN2, SEPTIN6 and SEPTIN7 that associate head-to-head to form a hexameric unit. Interaction between SEPTIN2 and SEPTIN7 seems indirect. Interacts with SEPTIN5 (By similarity). Interaction with SEPTIN4 not detected (By similarity). Interacts with SEPTIN9. Component of a septin core octomeric complex consisting of SEPTIN12, SEPTIN7, SEPTIN6 and SEPTIN2 or SEPTIN4 in the order 12-7-6-2-2-6-7-12 or 12-7-6-4-4-6-7-12 and located in the sperm annulus. Interacts with MAP4. Interacts with DZIP1L.

タンパク質ファミリー:

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.

研究領域

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

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