SEPT6 Antibody - #DF4459
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4459, RRID:AB_2836814.
折りたたみ／展開
KIAA0128; Nkrf; RCG53214, isoform CRA_d; RP5-876A24.2; SEP2; SEPT2; SEPT6; SEPT6/MLL FUSION GENE; SEPT6_HUMAN; Septin 2; Septin 6; Septin-6;
免疫原
- Q14141 SEPT6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAATDIARQVGEGCRTVPLAGHVGFDSLPDQLVNKSVSQGFCFNILCVGETGLGKSTLMDTLFNTKFEGEPATHTQPGVQLQSNTYDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVLHTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADAISKSELTKFKIKITSELVSNGVQIYQFPTDDESVAEINGTMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEDKKKSLDDEVNAFKQRKTAAELLQSQGSQAGGSQTLKRDKEKKNNPWLCTE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14141 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| Y295 | Phosphorylation | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| Y324 | Phosphorylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| K362 | Acetylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K367 | Acetylation | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| K379 | Acetylation | Uniprot | |
| K380 | Acetylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| K397 | Acetylation | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| T418 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| C432 | S-Nitrosylation | Uniprot |
研究背景
Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation.
Cytoplasm. Cytoplasm>Cytoskeleton>Spindle. Chromosome>Centromere>Kinetochore. Cleavage furrow. Midbody. Cell projection>Cilium>Flagellum.
Note: In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. Found in the sperm annulus (PubMed:25588830).
Widely expressed.
Septins polymerize into heterooligomeric protein complexes that form filaments, and associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation. Filaments are assembled from asymmetrical heterotrimers, composed of SEPTIN2, SEPTIN6 and SEPTIN7 that associate head-to-head to form a hexameric unit. Within the trimer, directly interacts with SEPTIN2 and SEPTIN7. Also interacts with SEPTIN9 and SEPTIN12. Interaction with SEPTIN12 alters filament structure. Component of a septin core octomeric complex consisting of SEPTIN12, SEPTIN7, SEPTIN6 and SEPTIN2 or SEPTIN4 in the order 12-7-6-2-2-6-7-12 or 12-7-6-4-4-6-7-12 and located in the sperm annulus. Interacts with SOCS7. Interacts with HNRNPA1.
(Microbial infection) Interacts with HCV NS5B.
Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.
研究領域
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.