NEK7 Antibody - #DF4467
(2)
(6)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4467, RRID:AB_2836822.
折りたたみ／展開
EC 2.7.11.1; NEK7; NEK7_HUMAN; Never in mitosis A-related kinase 7; Never in mitosis gene a-related kinase 7; NIMA (never in mitosis gene a) related kinase 7; NIMA (never in mitosis gene a)-related expressed kinase 7; NimA related protein kinase 7; NIMA-related kinase 7; NimA-related protein kinase 7; Serine/threonine-protein kinase Nek7;
免疫原
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
- Q8TDX7 NEK7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TDX7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| T30 | Phosphorylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| Y97 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| T190 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Q8TD19 (NEK9) | Uniprot |
| T199 | Phosphorylation | Uniprot | |
| Y201 | Phosphorylation | Uniprot | |
| Y202 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| Y244 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| Y287 | Phosphorylation | Uniprot | |
| Y289 | Phosphorylation | Uniprot | |
| K293 | Ubiquitination | Uniprot |
研究背景
Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.
Phosphorylation at Ser-195 required for its activation.
Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity).
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
Monomer. Interacts with NEK9. Interacts with ANKS3; this interaction alters the subcellular distribution of NEK7 by preventing its nuclear translocation.
Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.
The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity.
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
研究領域
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
参考文献
Application: WB Species: Human Sample: colon cancer tissues
Application: WB Species: Rat Sample: diabetic myocardium
Application: IHC Species: Rat Sample: diabetic myocardium
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