SLC6A3 Antibody - #DF4529

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製品: SLC6A3 Antibody
カタログ: DF4529
タンパク質の説明: Rabbit polyclonal antibody to SLC6A3
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 68 KD; 68kD(Calculated).
ユニプロット: Q01959
RRID: AB_2836880

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Dog(91%)
クローナリティ:
Polyclonal
特異性:
SLC6A3 Antibody detects endogenous levels of total SLC6A3.
RRID:
AB_2836880
引用形式: Affinity Biosciences Cat# DF4529, RRID:AB_2836880.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DA transporter; DAT 1; DAT; DAT1; Dopamine transporter 1; Dopamine transporter; PKDYS; SC6A3_HUMAN; SLC6A3; Sodium dependent dopamine transporter; Sodium-dependent dopamine transporter; Solute carrier family 6 (neurotransmitter transporter dopamine), member 3; Solute carrier family 6 (neurotransmitter transporter), member 3; Solute carrier family 6 member 3; Variable number tandem repeat (VNTR);

免疫原

免疫原:
Uniprot:

Q01959(SC6A3_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC6A3(6531)
発現特異性:
Q01959 SC6A3_HUMAN:

Highly expressed in substantia nigra.

タンパク質配列:
MSKSKCSVGLMSSVVAPAKEPNAVGPKEVELILVKEQNGVQLTSSTLTNPRQSPVEAQDRETWGKKIDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLLFMVIAGMPLFYMELALGQFNREGAAGVWKICPILKGVGFTVILISLYVGFFYNVIIAWALHYLFSSFTTELPWIHCNNSWNSPNCSDAHPGDSSGDSSGLNDTFGTTPAAEYFERGVLHLHQSHGIDDLGPPRWQLTACLVLVIVLLYFSLWKGVKTSGKVVWITATMPYVVLTALLLRGVTLPGAIDGIRAYLSVDFYRLCEASVWIDAATQVCFSLGVGFGVLIAFSSYNKFTNNCYRDAIVTTSINSLTSFSSGFVVFSFLGYMAQKHSVPIGDVAKDGPGLIFIIYPEAIATLPLSSAWAVVFFIMLLTLGIDSAMGGMESVITGLIDEFQLLHRHRELFTLFIVLATFLLSLFCVTNGGIYVFTLLDHFAAGTSILFGVLIEAIGVAWFYGVGQFSDDIQQMTGQRPSLYWRLCWKLVSPCFLLFVVVVSIVTFRPPHYGAYIFPDWANALGWVIATSSMAMVPIYAAYKFCSLPGSFREKLAYAIAPEKDRELVDRGEVRQFTLRHWLKV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Xenopus
67
Zebrafish
64
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01959 基板として

Site PTM Type Enzyme
S2 Phosphorylation
S4 Phosphorylation
K5 Ubiquitination
S7 Phosphorylation P17612 (PRKACA)
S12 Phosphorylation
S13 Phosphorylation
S53 Phosphorylation P28482 (MAPK1)
T62 Phosphorylation
T278 Phosphorylation
T286 Phosphorylation
Y593 Phosphorylation
T613 Phosphorylation

研究背景

機能:

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in substantia nigra.

サブユニット構造:

Homooligomer; disulfide-linked (Ref.14). Interacts with PRKCABP and TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2. Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location (By similarity). Interacts with alpha-synuclein/SNCA.

タンパク質ファミリー:

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.

研究領域

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Nervous system > Dopaminergic synapse.

参考文献

1). Integrative multilevel exploration of the mechanism by which Er-Zhi-Wan alleviates the Parkinson's disease (PD)-like phenotype in the MPTP-induced PD mouse model. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37348406) [IF=7.5]

Application: WB    Species: Mouse    Sample:

Fig. 3. EZW reduces the dopaminergic neuron damage in MPTP-PD mice. (A) The expression of TH, α-syn, and DAT in the striatum of each group was measured by Western blot analysis (n = 3 per group). (B) Relative densities of TH, α-syn, DAT, and β-actin were measured in different groups using densitometric analysis of Western blots. TH, α-syn, and DAT proteins were normalized to β-actin, and were expressed as the fold difference relative to the control group (Data represent the mean ± SD, n = 3. * P 

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