SNX1 Antibody - #DF4536
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4536, RRID:AB_2836887.
折りたたみ/展開
HsT17379; MGC8664; SNX 1; SNX 1a; Snx1; SNX1_HUMAN; SNX1A; Sorting nexin 1; Sorting nexin 1A; Sorting nexin-1; Vps5;
免疫原
- Q13596 SNX1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASGGGGCSASERLPPPFPGLEPESEGAAGGSEPEAGDSDTEGEDIFTGAAVVSKHQSPKITTSLLPINNGSKENGIHEEQDQEPQDLFADATVELSLDSTQNNQKKVLAKTLISLPPQEATNSSKPQPTYEELEEEEQEDQFDLTVGITDPEKIGDGMNAYVAYKVTTQTSLPLFRSKQFAVKRRFSDFLGLYEKLSEKHSQNGFIVPPPPEKSLIGMTKVKVGKEDSSSAEFLEKRRAALERYLQRIVNHPTMLQDPDVREFLEKEELPRAVGTQTLSGAGLLKMFNKATDAVSKMTIKMNESDIWFEEKLQEVECEEQRLRKLHAVVETLVNHRKELALNTAQFAKSLAMLGSSEDNTALSRALSQLAEVEEKIEQLHQEQANNDFFLLAELLSDYIRLLAIVRAAFDQRMKTWQRWQDAQATLQKKREAEARLLWANKPDKLQQAKDEILEWESRVTQYERDFERISTVVRKEVIRFEKEKSKDFKNHVIKYLETLLYSQQQLAKYWEAFLPEAKAIS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13596 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S3 | Phosphorylation | Uniprot | |
S25 | Phosphorylation | Uniprot | |
S32 | Phosphorylation | Uniprot | |
S39 | Phosphorylation | Uniprot | |
T41 | Phosphorylation | Uniprot | |
T48 | Phosphorylation | Uniprot | |
S54 | Phosphorylation | Uniprot | |
S58 | Phosphorylation | Uniprot | |
S64 | Phosphorylation | Uniprot | |
S72 | Phosphorylation | Uniprot | |
Y131 | Phosphorylation | Uniprot | |
Y162 | Phosphorylation | Uniprot | |
S172 | Phosphorylation | Uniprot | |
K179 | Ubiquitination | Uniprot | |
S188 | Phosphorylation | Uniprot | |
Y194 | Phosphorylation | Uniprot | |
K196 | Ubiquitination | Uniprot | |
K200 | Ubiquitination | Uniprot | |
S215 | Phosphorylation | Uniprot | |
T220 | Phosphorylation | Uniprot | |
K223 | Ubiquitination | Uniprot | |
K226 | Ubiquitination | Uniprot | |
K237 | Acetylation | Uniprot | |
K237 | Ubiquitination | Uniprot | |
R262 | Methylation | Uniprot | |
K267 | Ubiquitination | Uniprot | |
T276 | Phosphorylation | Uniprot | |
T278 | Phosphorylation | Uniprot | |
S280 | Phosphorylation | Uniprot | |
K286 | Ubiquitination | Uniprot | |
K290 | Ubiquitination | Uniprot | |
K297 | Ubiquitination | Uniprot | |
K325 | Ubiquitination | Uniprot | |
K349 | Ubiquitination | Uniprot | |
S368 | Phosphorylation | Uniprot | |
K442 | Ubiquitination | Uniprot | |
K445 | Ubiquitination | Uniprot | |
K450 | Ubiquitination | Uniprot | |
K490 | Ubiquitination | Uniprot | |
Y502 | Phosphorylation | Uniprot | |
K519 | Ubiquitination | Uniprot |
研究背景
Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors (IGF2R, M6PR and SORT1) and Shiginella dysenteria toxin stxB. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Involvement in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN. Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking.
Endosome membrane>Peripheral membrane protein>Cytoplasmic side. Golgi apparatus>trans-Golgi network membrane>Peripheral membrane protein>Cytoplasmic side. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium.
Note: Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PubMed:15498486). Colocalized with SORT1 to tubular endosomal membrane structures called endosome-to-TGN transport carriers (ETCs) which are budding from early endosome vacuoles just before maturing into late endosome vacuoles (PubMed:18088323). Colocalizes with DNAJC13 and Shiginella dysenteria toxin stxB on early endosomes (PubMed:19874558). Colocalized with F-actin at the leading edge of lamellipodia in a KALRN-dependent manner (PubMed:20604901).
Predominantly forms heterodimers with BAR domain-containing sorting nexins SNX5, SNX6 and SNX32; can self-associate to form homodimers. The heterodimers are proposed to self-assemble into helical arrays on the membrane to stabilize and expand local membrane curvature underlying endosomal tubule formation. Thought to be a component of the originally described retromer complex (also called SNX-BAR retromer) which is a pentamer containing the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS) and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity (Probable). Interacts with SNX5, SNX6, SNX32, VPS26A, VPS29, VPS35, DRD5, DENND5A, KALRN, RHOG (GDP-bound form). The interaction with SNX2 is reported controversially. Interacts with DNAJC13; prevented by presence of HGS. Interacts with HGS (By similarity).
The BAR domain is able to sense membrane curvature upon dimerization (PubMed:19816406). Membrane remodeling seems to implicate insertion of a N-terminal amphipatric helix (AH) in the membrane (Probable).
Belongs to the sorting nexin family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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