製品: SNX3 Antibody
カタログ: DF4537
タンパク質の説明: Rabbit polyclonal antibody to SNX3
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 18 KD; 19kD(Calculated).
ユニプロット: O60493
RRID: AB_2836888

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(91%)
クローナリティ:
Polyclonal
特異性:
SNX3 Antibody detects endogenous levels of total SNX3.
RRID:
AB_2836888
引用形式: Affinity Biosciences Cat# DF4537, RRID:AB_2836888.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Grd19; MCOPS8; MGC151262; MGC151266; MGC17570; OTTHUMP00000016941; OTTHUMP00000046475; OTTHUMP00000046476; Protein SDP3; SDP3; SNX3; SNX3_HUMAN; SNX3A; Sorting nexin 3; Sorting nexin 3A; Sorting nexin-3;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MAETVADTRRLITKPQNLNDAYGPPSNFLEIDVSNPQTVGVGRGRFTTYEIRVKTNLPIFKLKESTVRRRYSDFEWLRSELERESKVVVPPLPGKAFLRQLPFRGDDGIFDDNFIEERKQGLEQFINKVAGHPLAQNERCLHMFLQDEIIDKSYTPSKIRHA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
82
Chicken
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60493 基板として

Site PTM Type Enzyme
Ubiquitination
A2 Acetylation
K14 Ubiquitination
Y22 Phosphorylation
S26 Phosphorylation
R43 Methylation
R45 Methylation
T47 Phosphorylation
T48 Phosphorylation
Y49 Phosphorylation
K54 Ubiquitination
T55 Phosphorylation
K61 Ubiquitination
K63 Ubiquitination
T66 Phosphorylation
Y71 Phosphorylation
S72 Phosphorylation
S79 Phosphorylation
K86 Sumoylation
K86 Ubiquitination
K95 Sumoylation
K95 Ubiquitination
K119 Sumoylation
K119 Ubiquitination
K128 Sumoylation
K128 Ubiquitination
K152 Ubiquitination
S153 Phosphorylation
Y154 Phosphorylation
T155 Phosphorylation
K158 Sumoylation
K158 Ubiquitination

研究背景

機能:

Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Also can bind phosphatidylinositol 4-phosphate (PtdIns(P4)), phosphatidylinositol 5-phosphate (PtdIns(P5)) and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2) (By similarity). Plays a role in protein transport between cellular compartments. Together with RAB7A facilitates endosome membrane association of the retromer cargo-selective subcomplex (CSC/VPS). May in part act as component of the SNX3-retromer complex which mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G (By similarity). Not involved in EGFR degradation. Involved in the regulation of phagocytosis in dendritic cells possibly by regulating EEA1 recruitment to the nascent phagosomes. Involved in iron homeostasis through regulation of endocytic recycling of the transferrin receptor TFRC presumably by delivering the transferrin:transferrin receptor complex to recycling endosomes; the function may involve the CSC retromer subcomplex (By similarity). In the case of Salmonella enterica infection plays arole in maturation of the Salmonella-containing vacuole (SCV) and promotes recruitment of LAMP1 to SCVs.

PTMs:

Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP10 (By similarity).

細胞の位置付け:

Early endosome. Cytoplasmic vesicle>Phagosome.
Note: Colocalizes to clathrin-coated endosomal vesicles morphologically distinct from retromer-decorated non-branched endosomal tubule structures (PubMed:21725319) Colocalizes with EEA1 on nascent phagosomes in dendritic cells but competes with EEA1 for binding to phagosomal membrane (PubMed:23237080). In the case of Salmonella enterica infection localizes to Salmonella-containing vacuoles (SCVs) from which SNX3-containing tubules form 30-60 min after infection (PubMed:20482551).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with VPS26A, VPS29 and VPS35; the interaction with VPS35 is direct. The association with the retromer CSC subcomplex subunits is proposed to represent a functional distinct retromer variant described as SNX3-retromer complex. Interacts with USP10 and SCNN1A (By similarity). Interacts with TRFC (By similarity). Interacts with SNX8; 2 molecules of SNX8 seems to associate with one molecule of SNX3. Interacts with PTPRU.

タンパク質ファミリー:

The PX domain mediates specific binding to phosphatidylinositol 3-phosphate (PtdIns(P3)).

Belongs to the sorting nexin family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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