SF3B4 Antibody - #DF4540
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4540, RRID:AB_2836891.
折りたたみ／展開
AFD1; Hsh49; MGC10828; Pre mRNA splicing factor SF3b 49 kDa subunit; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SAP 49; SAP49; Sf3b4; SF3B4_HUMAN; SF3b49; SF3b50; Spliceosomal protein; Spliceosome associated protein (U2 snRNP); Spliceosome associated protein 49; Spliceosome-associated protein 49; Splicing factor 3b subunit 4 49kDa; Splicing factor 3B subunit 4;
免疫原
- Q15427 SF3B4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADAPPPPSAPNPVVSSLGSGLPPPGMPPPGSFPPPVPPPGALPPGIPPAMPPPPMPPGAAGHGPPSAGTPGAGHPGHGHSHPHPFPPGGMPHPGMSQMQLAHHGPHGLGHPHAGPPGSGGQPPPRPPPGMPHPGPPPMGMPPRGPPFGSPMGHPGPMPPHGMRGPPPLMPPHGYTGPPRPPPYGYQRGPLPPPRPTPRPPVPPRGPLRGPLPQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15427 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| Y16 | Phosphorylation | Uniprot | |
| T42 | Phosphorylation | Uniprot | |
| Y56 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| Y69 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| Y117 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| R390 | Methylation | Uniprot | |
| R398 | Methylation | Uniprot | |
| R409 | Methylation | Uniprot | |
| R415 | Methylation | Uniprot | |
| R419 | Methylation | Uniprot |
研究背景
Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Nucleus.
Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts directly with SF3B2. Found in a complex with PRMT9, SF3B2 and SF3B4. The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome.
Belongs to the SF3B4 family.
研究領域
· Genetic Information Processing > Transcription > Spliceosome.
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