ZIP7 Antibody - #DF4635

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製品: ZIP7 Antibody
カタログ: DF4635
タンパク質の説明: Rabbit polyclonal antibody to ZIP7
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 50 KD; 50kD(Calculated).
ユニプロット: Q92504
RRID: AB_2836926

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MS Report
HPLC Report
MSDS
説明書
COA
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
ZIP7 Antibody detects endogenous levels of total ZIP7.
RRID:
AB_2836926
引用形式: Affinity Biosciences Cat# DF4635, RRID:AB_2836926.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

D6S115E; D6S2244E; H2 KE4; Histidine rich membrane protein Ke4; Histidine-rich membrane protein Ke4; HKE4; HLA class II region expressed gene KE4; KE4; Ke4 gene mouse, human homolog of; Really interesting new gene 5 protein; RING5; S39A7_HUMAN; SLC39A7; Solute carrier family 39 (zinc transporter), member 7; Solute carrier family 39 member 7; solute carrier family 39 zinc transporter member 7; Zinc transporter SLC39A7; ZIP7;

免疫原

免疫原:

A synthesized peptide derived from human ZIP7, corresponding to a region within the internal amino acids.

Uniprot:

Q92504(S39A7_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC39A7(7922)
発現特異性:
Q92504 S39A7_HUMAN:

Widely expressed.

タンパク質配列:
MARGLGAPHWVAVGLLTWATLGLLVAGLGGHDDLHDDLQEDFHGHSHRHSHEDFHHGHSHAHGHGHTHESIWHGHTHDHDHGHSHEDLHHGHSHGYSHESLYHRGHGHDHEHSHGGYGESGAPGIKQDLDAVTLWAYALGATVLISAAPFFVLFLIPVESNSPRHRSLLQILLSFASGGLLGDAFLHLIPHALEPHSHHTLEQPGHGHSHSGQGPILSVGLWVLSGIVAFLVVEKFVRHVKGGHGHSHGHGHAHSHTRGSHGHGRQERSTKEKQSSEEEEKETRGVQKRRGGSTVPKDGPVRPQNAEEEKRGLDLRVSGYLNLAADLAHNFTDGLAIGASFRGGRGLGILTTMTVLLHEVPHEVGDFAILVQSGCSKKQAMRLQLLTAVGALAGTACALLTEGGAVGSEIAGGAGPGWVLPFTAGGFIYVATVSVLPELLREASPLQSLLEVLGLLGGVIMMVLIAHLE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Sheep
91
Rabbit
91
Xenopus
64
Zebrafish
45
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Zinc transporter, that transports Zn(2+) from the endoplasmic reticulum/Golgi apparatus to the cytosol. Transport is stimulated by growth factors, such as EGF, and Ca(2+), as well as by exogenous Zn(2+).

PTMs:

Rapidly phosphorylated by CK2 following Zn(2+) treatment. This phosphorylation is required for efficient cytosolic Zn(2+) release.

細胞の位置付け:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus>cis-Golgi network membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed.

タンパク質ファミリー:

Belongs to the ZIP transporter (TC 2.A.5) family. KE4/Catsup subfamily.

参考文献

1). The NLRX1-SLC39A7 complex orchestrates mitochondrial dynamics and mitophagy to rejuvenate intervertebral disc by modulating mitochondrial Zn2+ trafficking. Autophagy, 2024 (PubMed: 37876250) [IF=14.6]
2). SYNJ2BP ameliorates intervertebral disc degeneration by facilitating mitochondria-associated endoplasmic reticulum membrane formation and mitochondrial Zn2+ homeostasis. Free radical biology & medicine, 2024 (PubMed: 38158052) [IF=7.1]

Application: WB    Species: human    Sample: NP cell

Fig. 2. MAM structure is essential for SLC39A7-NLRX1 complex formation and NP cell proliferation by maintaining mitochondrial Zn2+ homeostasis. (A) Confocal analysis of TOMM20 and CANX with IF staining in human NP cells isolated from health NP tissues following the treatments of PBS or TBHP with MFN2 knockdown or not, scale bar: 10 μm. (B) MAM structure analysis by TEM in human NP cells isolated from health NP tissues following the treatments of PBS or TBHP with MFN2 knockdown or not, scale bar: 1 μm. (C) Endogenous protein immunoprecipitated (IP) from NP cells isolated from health NP tissues with MFN2 knockdown or not, followed by western blotting, IP with anti-NLRX1 antibody. (D) Protein lysates extracted specially in mitochondria and cytoplasm of human NP cells isolated from health NP tissues with MFN2 knockdown or not, followed by western blotting. (E) Zinc distribution in primary human NP cells isolated from health NP tissues with MFN2 knockdown or not, detected by FluoZin™-3-AM labelling and confocal fluorescence microscope, scale bar: 10 μm. (F) Mitochondrial morphology analysis by fluorescence microscope with MitoTracker Red CMXRos label in primary human NP cells isolated from health NP tissues following the treatments of PBS or TBHP with MFN2 knockdown or not, scale bar: 5 μm. (G, H) Protein expressions of cell senescence indicators (TP53, CDKN2A) and extracellular matrix (COL2A1) in primary human NP cells isolated from health NP tissues following the treatments of PBS or TBHP with MFN2 knockdown or not, as determined by western blotting. (I-K) Cell senescence (SA-GLB1/β-gal staining) and cell proliferation (EdU incorporation) in primary human NP cells isolated from health NP tissues following the treatments of PBS or TBHP with MFN2 knockdown or not, scale bar: 100 μm (white light images), 200 μm (IF images). Data are represented as mean ± SD. *p 

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