SLC39A4 Antibody - #DF4638

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製品: SLC39A4 Antibody
カタログ: DF4638
タンパク質の説明: Rabbit polyclonal antibody to SLC39A4
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human
予測: Pig, Bovine, Horse, Dog
分子量: 68 KD; 68kD(Calculated).
ユニプロット: Q6P5W5
RRID: AB_2836929

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(80%), Bovine(80%), Horse(83%), Dog(83%)
クローナリティ:
Polyclonal
特異性:
SLC39A4 Antibody detects endogenous levels of total SLC39A4.
RRID:
AB_2836929
引用形式: Affinity Biosciences Cat# DF4638, RRID:AB_2836929.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

1600025H15Rik; Acrodermatitis enteropathica zinc deficiency type; Activated in W/Wv mouse stomach 2; AEZ; AU041686; AWMS2; FLJ20327; MGC156705; MGC74741; S39A4_HUMAN; Slc39a4; Solute carrier family 39 member 4; Ssolute carrier family 39 zinc transporter member 4; Zinc transporter ZIP4; ZIP-4; ZIP4; Zrt and Irt like protein 4; Zrt- and Irt-like protein 4;

免疫原

免疫原:

A synthesized peptide derived from human SLC39A4, corresponding to a region within the internal amino acids.

Uniprot:

Q6P5W5(S39A4_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC39A4(55630)
発現特異性:
Q6P5W5 S39A4_HUMAN:

Highly expressed in kidney, small intestine, stomach, colon, jejunum and duodenum.

タンパク質配列:
MASLVSLELGLLLAVLVVTATASPPAGLLSLLTSGQGALDQEALGGLLNTLADRVHCANGPCGKCLSVEDALGLGEPEGSGLPPGPVLEARYVARLSAAAVLYLSNPEGTCEDARAGLWASHADHLLALLESPKALTPGLSWLLQRMQARAAGQTPKMACVDIPQLLEEAVGAGAPGSAGGVLAALLDHVRSGSCFHALPSPQYFVDFVFQQHSSEVPMTLAELSALMQRLGVGREAHSDHSHRHRGASSRDPVPLISSSNSSSVWDTVCLSARDVMAAYGLSEQAGVTPEAWAQLSPALLQQQLSGACTSQSRPPVQDQLSQSERYLYGSLATLLICLCAVFGLLLLTCTGCRGVTHYILQTFLSLAVGAVTGDAVLHLTPKVLGLHTHSEEGLSPQPTWRLLAMLAGLYAFFLFENLFNLLLPRDPEDLEDGPCGHSSHSHGGHSHGVSLQLAPSELRQPKPPHEGSRADLVAEESPELLNPEPRRLSPELRLLPYMITLGDAVHNFADGLAVGAAFASSWKTGLATSLAVFCHELPHELGDFAALLHAGLSVRQALLLNLASALTAFAGLYVALAVGVSEESEAWILAVATGLFLYVALCDMLPAMLKVRDPRPWLLFLLHNVGLLGGWTVLLLLSLYEDDITF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
83
Dog
83
Pig
80
Bovine
80
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Plays an important role in cellular zinc homeostasis as a zinc transporter. Regulated in response to zinc availability (By similarity).

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Recycling endosome membrane>Multi-pass membrane protein.
Note: Colocalized with TFRC in the recycling endosomes. Cycles between endosomal compartments and the plasma membrane in response to zinc availability.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in kidney, small intestine, stomach, colon, jejunum and duodenum.

タンパク質ファミリー:

Belongs to the ZIP transporter (TC 2.A.5) family.

研究領域

· Organismal Systems > Digestive system > Mineral absorption.

参考文献

1). UKLF/PCBP2 axis governs the colorectal cancer development by transcriptionally activating SLC39A4. Biochimica et biophysica acta. Molecular cell research, 2024 (PubMed: 38768927) [IF=4.6]

Application: WB    Species: human    Sample: CRC tissues

Fig. 8. UKLF activates the transcription of SLC39A4. (A) A positive correlation was observed between UKLF and SLC39A4 expression in CRC tissues. HCT116 and SW480 cells were transfected with shUKLFGFP or OV-UKLFGFP to construct UKLF knockdown or overexpression cell lines, (B) the mRNA and (C–D) protein expression of SLC39A4 was measured. (E) Analysis of UKLF binding sites in the SLC39A4 promoter by JASPAR database. (F) HCT116 cells were co-transfected with SLC39A4 promoter luciferase reporter plasmids and UKLF overexpression plasmids (OV-UKLF), 48 h post-transfection, the relationship between UKLF and SLC39A4 was determined by a luciferase reporter assay. (G) The binding of UKLF to the promoter regions of SLC39A4 was determined by chromatin immunoprecipitation. HCT116 were co-transfected with OV-UKLFGFP and shSLC39A4GFP, 48 h post-transfection, the mRNA expression of (H) UKLF and (I) SLC39A4 was detected by qRT-PCR. (J) The viability was measured with the MTT assay. Values were mean ± SD. *p < 0.05, **p < 0.01. Cell experiments were performed at least three biological repetitions per group.

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