APOA1 Antibody - #BF0578

(1)  
製品: APOA1 Antibody
カタログ: BF0578
タンパク質の説明: Mouse monoclonal antibody to APOA1
アプリケーション: WB ELISA
反応性: Human
分子量: 25kDa; 31kD(Calculated).
ユニプロット: P02647
RRID: AB_2833629

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説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Mouse
アプリケーション:
ELISA 1:10000, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFB1605]
特異性:
APOA1 antibody detects endogenous levels of total APOA1.
RRID:
AB_2833629
引用形式: Affinity Biosciences Cat# BF0578, RRID:AB_2833629.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Apo-AI; ApoA I; ApoA-I; APOA1; APOA1_HUMAN; Apolipoprotein A-I(1-242); Apolipoprotein A1; Apolipoprotein AI; Brp14; Ltw1; Lvtw1; Sep1; Sep2;

免疫原

免疫原:

Purified recombinant fragment of human APOA1 expressed in E. Coli.

Uniprot:

P02647(APOA1_HUMAN) >>Visit HPA database.

遺伝子(ID):
APOA1(335)
発現特異性:
P02647 APOA1_HUMAN:

Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.

タンパク質の説明:
APOA1: apolipoprotein A-I, it is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.
タンパク質配列:
MKAAVLTLAVLFLTGSQARHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ

PTMs - P02647 基板として

Site PTM Type Enzyme
Y53 Phosphorylation
S55 Phosphorylation
S60 Phosphorylation
K130 Acetylation
K142 Ubiquitination
S166 Phosphorylation
T185 Phosphorylation
Y190 Phosphorylation
S191 Phosphorylation
Y216 Phosphorylation
K219 Acetylation
T221 O-Glycosylation
T221 Phosphorylation
S225 Phosphorylation
T226 Phosphorylation
S228 O-Glycosylation
S252 Phosphorylation
K262 Acetylation

研究背景

機能:

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

PTMs:

Glycosylated.

Palmitoylated.

Phosphorylation sites are present in the extracellular medium.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.

サブユニット構造:

Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Interacts with SCGB3A2.

タンパク質ファミリー:

Belongs to the apolipoprotein A1/A4/E family.

研究領域

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Digestive system > Fat digestion and absorption.

· Organismal Systems > Digestive system > Vitamin digestion and absorption.

· Organismal Systems > Digestive system > Cholesterol metabolism.

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