製品: EIF2B4 Antibody
カタログ: DF4577
タンパク質の説明: Rabbit polyclonal antibody to EIF2B4
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 57 KD; 58kD(Calculated).
ユニプロット: Q9UI10
RRID: AB_2836941

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(89%)
クローナリティ:
Polyclonal
特異性:
EIF2B4 Antibody detects endogenous levels of total EIF2B4.
RRID:
AB_2836941
引用形式: Affinity Biosciences Cat# DF4577, RRID:AB_2836941.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

EI2BD_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor subunit delta; eIF-2B GDP-GTP exchange factor subunit delta; Eif2b4; EIF2Bdelta; eukaryotic translation initiation factor 2B subunit 4; Translation initiation factor eIF-2B subunit delta;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MAAVAVAVREDSGSGMKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPETGSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKGEQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSLVSRILQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGEHVALANWQNHASLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLRVKSSDQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
89
Zebrafish
89
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UI10 基板として

Site PTM Type Enzyme
A2 Acetylation
S12 Phosphorylation
C69 S-Nitrosylation
T86 Phosphorylation
S130 Phosphorylation
T137 Phosphorylation
K142 Ubiquitination
Y147 Phosphorylation
K176 Ubiquitination
Y186 Phosphorylation
S187 Phosphorylation
S191 Phosphorylation
Y214 Phosphorylation
K282 Ubiquitination
K291 Ubiquitination
K297 Ubiquitination
S328 Phosphorylation
S340 Phosphorylation
R374 Methylation
S384 Phosphorylation
Y385 Phosphorylation
S399 Phosphorylation
K442 Ubiquitination
C465 S-Nitrosylation
K466 Acetylation
K466 Ubiquitination

研究背景

機能:

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

サブユニット構造:

Complex of five different subunits; alpha, beta, gamma, delta and epsilon.

タンパク質ファミリー:

Belongs to the eIF-2B alpha/beta/delta subunits family.

研究領域

· Genetic Information Processing > Translation > RNA transport.

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