SGK269 Antibody - #DF4583
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4583, RRID:AB_2836947.
折りたたみ／展開
FLJ21140; FLJ34483; KIAA2002; NKF3 kinase family member; PEAK1; PEAK1_HUMAN; pseudopodium enriched atypical kinase; Pseudopodium-enriched atypical kinase 1; SGK269; Sugen kinase 269; Tyrosine-protein kinase SgK269;
免疫原
- Q9H792 PEAK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNVKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMIVADGQSICGELSIQEHCENKPVIIGWNRNRAALSQKPLNNNNEDDEGISHVPKPYGNNDSAKKMSDNNNGLTEVLKEIAGLDTAPQIRGNETNSRETFLGRINDCYKRSLERKLPPSCMIGGIKETQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLFSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTIPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGQGSIQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGLSPGNPGDSKDMKEIEPNYESPSSNNQDKDSSQASKSSIKVPETHKAVLALRLEEKDGKIAVQTEKEESKASTDVAGQAVTINLVPTEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGPVNSPKTKSSSSTPNSPVTSSSLTPGQISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELITSGTGPNVPPRKNCHKSAPTSPTATNISSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKVPDNTTSKTTDCLQTKGFSNSTEHKRGSVAQKVQEFNNCLNRGQSSPQRSYSSSHSSPAKIQRATQEPVAKIEGTQESQMVGSSSTREKASTVLSQIVASIQPPQSPPETPQSGPKACSVEELYAIPPDADVAKSTPKSTPVRPKSLFTSQPSGEAEAPQTTDSPTTKVQKDPSIKPVTPSPSKLVTSPQSEPPAPFPPPRSTSSPYHAGNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKEREKGKLVGLDGTVIHMLPPPPVQRHHWFTEAKGESSEKPAIVFMYRCDPAQGQLSVDQSKARTDQAAVMEKGRAENALLQDSEKKRSHSSPSQIPKKILSHMTHEVTEDFSPRDPRTVVGKQDGRGCTSVTTALSLPELEREDGKEDISDPMDPNPCSATYSNLGQSRAAMIPPKQPRQPKGAVDDAIAFGGKTDQEAPNASQPTPPPLPKKMIIRANTEPISKDLQKSMESSLCVMANPTYDIDPNWDASSAGSSISYELKGLDIESYDSLERPLRKERPVPSAANSISSLTTLSIKDRFSNSMESLSSRRGPSCRQGRGIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAVRQSLAVHFNIQQDCGHFLAEVPNRLLPWEDPDDPEKDEDDMEETEEDAKGETDGKNPKPCSEAASSQKENQGVMSKKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDLYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGTAQGFGPAEPSPTSSYPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEREYTRADLPRIPFRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTACPSLVQRNTLLQNWLDIKRTLLMIKFAEKSLDREGGISLEDWLCAQYLAFATTDSLSCIVKILQHR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H792 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S212 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| T293 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| Y462 | Phosphorylation | Uniprot | |
| Y475 | Phosphorylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| T504 | Phosphorylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| T510 | Phosphorylation | Uniprot | |
| T515 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| Y531 | Phosphorylation | Uniprot | |
| T539 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| K563 | Acetylation | Uniprot | |
| K567 | Acetylation | Uniprot | |
| S568 | Phosphorylation | Uniprot | |
| T571 | Phosphorylation | Uniprot | |
| S572 | Phosphorylation | Uniprot | |
| T574 | Phosphorylation | Uniprot | |
| T576 | Phosphorylation | Uniprot | |
| K581 | Acetylation | Uniprot | |
| S587 | Phosphorylation | Uniprot | |
| Y598 | Phosphorylation | Uniprot | |
| T615 | Phosphorylation | Uniprot | |
| Y616 | Phosphorylation | Uniprot | |
| Y635 | Phosphorylation | P07948 (LYN) | Uniprot |
| Y641 | Phosphorylation | Uniprot | |
| S643 | Phosphorylation | Uniprot | |
| S648 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| T657 | Phosphorylation | Uniprot | |
| Y665 | Phosphorylation | Uniprot | |
| S725 | Phosphorylation | Uniprot | |
| S729 | Phosphorylation | Uniprot | |
| S730 | Phosphorylation | Uniprot | |
| T759 | Phosphorylation | Uniprot | |
| S779 | Phosphorylation | Uniprot | |
| T783 | Phosphorylation | Uniprot | |
| S786 | Phosphorylation | Uniprot | |
| S792 | Phosphorylation | Uniprot | |
| Y797 | Phosphorylation | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| T813 | Phosphorylation | Uniprot | |
| S819 | Phosphorylation | Uniprot | |
| T822 | Phosphorylation | Uniprot | |
| S826 | Phosphorylation | Uniprot | |
| T834 | Phosphorylation | Uniprot | |
| S837 | Phosphorylation | Uniprot | |
| S847 | Phosphorylation | Uniprot | |
| T852 | Phosphorylation | Uniprot | |
| S854 | Phosphorylation | Uniprot | |
| S856 | Phosphorylation | Uniprot | |
| T860 | Phosphorylation | Uniprot | |
| S861 | Phosphorylation | Uniprot | |
| S864 | Phosphorylation | Uniprot | |
| T876 | Phosphorylation | Uniprot | |
| S877 | Phosphorylation | Uniprot | |
| S878 | Phosphorylation | Uniprot | |
| Y880 | Phosphorylation | Uniprot | |
| T897 | Phosphorylation | Uniprot | |
| S898 | Phosphorylation | Uniprot | |
| T900 | Phosphorylation | Uniprot | |
| K921 | Acetylation | Uniprot | |
| S927 | Phosphorylation | Uniprot | |
| T937 | Phosphorylation | Uniprot | |
| T975 | Phosphorylation | Uniprot | |
| S981 | Phosphorylation | Uniprot | |
| S1001 | Phosphorylation | Uniprot | |
| S1028 | Phosphorylation | Uniprot | |
| S1035 | Phosphorylation | Uniprot | |
| S1036 | Phosphorylation | Uniprot | |
| S1038 | Phosphorylation | Uniprot | |
| S1046 | Phosphorylation | Uniprot | |
| S1057 | Phosphorylation | Uniprot | |
| T1074 | Phosphorylation | Uniprot | |
| T1077 | Phosphorylation | Uniprot | |
| T1078 | Phosphorylation | Uniprot | |
| S1104 | Phosphorylation | Uniprot | |
| T1106 | Phosphorylation | Uniprot | |
| Y1107 | Phosphorylation | Uniprot | |
| T1151 | Phosphorylation | Uniprot | |
| T1165 | Phosphorylation | Uniprot | |
| Y1188 | Phosphorylation | Uniprot | |
| S1214 | Phosphorylation | Uniprot | |
| Y1215 | Phosphorylation | Uniprot | |
| S1217 | Phosphorylation | Uniprot | |
| S1234 | Phosphorylation | Uniprot | |
| S1248 | Phosphorylation | Uniprot | |
| S1250 | Phosphorylation | Uniprot | |
| S1253 | Phosphorylation | Uniprot | |
| S1256 | Phosphorylation | Uniprot | |
| S1292 | Phosphorylation | Uniprot | |
| S1347 | Phosphorylation | Uniprot | |
| Y1348 | Phosphorylation | Uniprot | |
| Y1371 | Phosphorylation | Uniprot | |
| Y1372 | Phosphorylation | Uniprot | |
| S1374 | Phosphorylation | Uniprot | |
| S1563 | Phosphorylation | Uniprot | |
| T1583 | Phosphorylation | Uniprot | |
| Y1587 | Phosphorylation | Uniprot | |
| S1657 | Phosphorylation | Uniprot |
研究背景
Probable catalytically inactive kinase. Scaffolding protein that regulates the cytoskeleton to control cell spreading and migration by modulating focal adhesion dynamics. Acts as a scaffold for mediating EGFR signaling.
Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Phosphorylation at Tyr-665 by a Src family kinase controls subcellular localization to focal adhesion and focal adhesion dynamics. Phosphorylation at Tyr-1188 is essential for binding to SHC1.
Cytoplasm>Cytoskeleton. Cell junction>Focal adhesion.
Note: Colocalizes with F-actin in serum-rich medium (PubMed:20534451). Actin colocalization is reduced during serum starvation (PubMed:20534451).
Homodimer. Interacts with BCAR1 and CRK. Interacts with PRAG1. Interacts (when phosphorylated at Tyr-1188) with SHC1 (via PID domain). Found in a complex with PPP1CA, PPP1CC, SHC1 and PEAK1.
The dimerization region encompasses helices both from the N- and C-terminal of the protein kinase domain.
Belongs to the protein kinase superfamily.
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