YOD1 Antibody - #DF4597
| 製品: | YOD1 Antibody |
| カタログ: | DF4597 |
| タンパク質の説明: | Rabbit polyclonal antibody to YOD1 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 分子量: | 35 KD; 38kD(Calculated). |
| ユニプロット: | Q5VVQ6 |
| RRID: | AB_2836961 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4597, RRID:AB_2836961.
折りたたみ／展開
YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) edit item name - YOD1 OTU deubiquinating enzyme 1, S. cerevisiae, homolog of; DUBA-8; DUBA8; HIN-7; HIV-1-induced protease 7; HsHIN7; OTU domain containing 2; OTU domain-containing protein 2; OTU1_HUMAN; OTUD2; PRO0907; RP11-164O23.1; Ubiquitin thioesterase OTU1; yod1; YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae); YOD1 OTU deubiquinating enzyme 1 homolog (yeast);
免疫原
- Q5VVQ6 OTU1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFGPAKGRHFGVHPAPGFPGGVSQQAAGTKAGPAGAWPVGSRTDTMWRLRCKAKDGTHVLQGLSSRTRVRELQGQIAAITGIAPGGQRILVGYPPECLDLSNGDTILEDLPIQSGDMLIIEEDQTRPRSSPAFTKRGASSYVRETLPVLTRTVVPADNSCLFTSVYYVVEGGVLNPACAPEMRRLIAQIVASDPDFYSEAILGKTNQEYCDWIKRDDTWGGAIEISILSKFYQCEICVVDTQTVRIDRFGEDAGYTKRVLLIYDGIHYDPLQRNFPDPDTPPLTIFSSNDDIVLVQALELADEARRRRQFTDVNRFTLRCMVCQKGLTGQAEAREHAKETGHTNFGEV
PTMs - Q5VVQ6 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S130 | Phosphorylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K325 | Ubiquitination | Uniprot |
研究背景
Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin. May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes. May recruit PLAA, UBXN6 and VCP to damaged lysosome membranes decorated with K48-linked ubiquitin chains and remove these chains allowing autophagosome formation.
Cytoplasm.
Note: Recruited to damaged lysosomes decorated with K48-linked ubiquitin chains.
Interacts with VCP; the interaction is direct. Interacts with FAF2/UBXD8. Interacts with DERL1; however interaction is dependent on the UBAX-like region, suggesting that it may be indirect. Interacts with PLAA, UBXN6 and VCP; may form a complex involved in macroautophagy.
The UBAX-like region mediates the interaction with VCP. According to PubMed:19818707, it corresponds to a UBX domain, which is a hallmark for VCP-associated proteins. However, no canonical UBX is detected by prediction tools such as Pfam or PROSITE.
The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains (PubMed:23827681).
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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