UBE2T Antibody - #DF4601
| 製品: | UBE2T Antibody |
| カタログ: | DF4601 |
| タンパク質の説明: | Rabbit polyclonal antibody to UBE2T |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 22 KD; 23kD(Calculated). |
| ユニプロット: | Q9NPD8 |
| RRID: | AB_2836965 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4601, RRID:AB_2836965.
折りたたみ／展開
Cell proliferation inducing gene 50 protein; Cell proliferation-inducing gene 50 protein; HSPC150; HSPC150 protein similar to ubiquitin conjugating enzyme; PIG50; Ube2t; UBE2T_HUMAN; Ubiquitin carrier protein T; Ubiquitin conjugating enzyme; ubiquitin conjugating enzyme E2 T; Ubiquitin conjugating enzyme E2T; Ubiquitin protein ligase T; Ubiquitin-conjugating enzyme E2 T; Ubiquitin-protein ligase T;
免疫原
- Q9NPD8 UBE2T_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NPD8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K28 | Ubiquitination | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| T72 | Phosphorylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| K191 | Acetylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot |
研究背景
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.
Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-182 are unclear: according to a report, monoubiquitination inactivates E2 enzyme activity. In contrast, according to another report, autoubiquitination does not affect E2 enzyme activity.
Nucleus.
Note: Accumulates to chromatin.
Directly interacts with FANCL. Interacts with BRCA1.
Belongs to the ubiquitin-conjugating enzyme family.
研究領域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
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