CACNA1H Antibody - #DF4615

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製品: CACNA1H Antibody
カタログ: DF4615
タンパク質の説明: Rabbit polyclonal antibody to CACNA1H
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Dog
分子量: 259 KD; 259kD(Calculated).
ユニプロット: O95180
RRID: AB_2836979

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
CACNA1H Antibody detects endogenous levels of total CACNA1H.
RRID:
AB_2836979
引用形式: Affinity Biosciences Cat# DF4615, RRID:AB_2836979.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Alpha1 3.2; Alpha13.2; CAC1H_HUMAN; CACNA 1H; CACNA1 H; CACNA1 HB; Cacna1h; CACNA1HB; Calcium channel alpha13.2 subunit; Calcium channel voltage dependent T type alpha 1H subunit; Calcium channel, voltage-dependent, T type, alpha 1Hb subunit; Cav 3.2; Cav T.2; Cav3.2; CavT.2; EIG 6; EIG6; Low voltage activated calcium channel alpha 13.2 subunit; Low voltage activated calcium channel alpha1 3.2 subunit; Low-voltage-activated calcium channel alpha1 3.2 subunit; MNCb 1209; T type Cav3.2; Voltage dependent t type calcium channel alpha 1H subunit; Voltage gated calcium channel alpha subunit Cav 3.2; Voltage gated calcium channel alpha subunit Cav T.2; Voltage gated calcium channel alpha subunit Cav3.2; Voltage gated calcium channel alpha subunit CavT.2; Voltage-dependent T-type calcium channel subunit alpha-1H; Voltage-gated calcium channel subunit alpha Cav3.2;

免疫原

免疫原:
Uniprot:

O95180(CAC1H_HUMAN) >>Visit HPA database.

遺伝子(ID):
CACNA1H(8912)
発現特異性:
O95180 CAC1H_HUMAN:

In nonneuronal tissues, the highest expression levels are found in the kidney, liver, and heart. In the brain, most abundant in the amygdala, caudate nucleus, and putamen (PubMed:9670923, PubMed:9930755). In the heart, expressed in blood vessels. Isoform 1 and isoform 2 are expressed in testis, primarily in the germ cells, but not in other portions of the reproductive tract, such as ductus deferens. Isoform 2 is not detected in brain (PubMed:11751928). Expressed in the adrenal glomerulosa (at protein level) (PubMed:25907736, PubMed:27729216).

タンパク質配列:
MTEGARAADEVRVPLGAPPPGPAALVGASPESPGAPGREAERGSELGVSPSESPAAERGAELGADEEQRVPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHVSMLVIMLNCVTLGMFRPCEDVECGSERCNILEAFDAFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVVAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQTEEGEENPFICSSRRDNGMQKCSHIPGRRELRMPCTLGWEAYTQPQAEGVGAARNACINWNQYYNVCRSGDSNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRARHLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSAVQGQGPGHRQRRAGRHTASVHHLVYHHHHHHHHHYHFSHGSPRRPGPEPGACDTRLVRAGAPPSPPSPGRGPPDAESVHSIYHADCHIEGPQERARVAHAAATAAASLRLATGLGTMNYPTILPSGVGSGKGSTSPGPKGKWAGGPPGTGGHGPLSLNSPDPYEKIPHVVGEHGLGQAPGHLSGLSVPCPLPSPPAGTLTCELKSCPYCTRALEDPEGELSGSESGDSDGRGVYEFTQDVRHGDRWDPTRPPRATDTPGPGPGSPQRRAQQRAAPGEPGWMGRLWVTFSGKLRRIVDSKYFSRGIMMAILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIIVVISVWEIVGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLVKTMDNVATFCTLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSDTDEDKTSVHFEEDFHKLRELQTTELKMCSLAVTPNGHLEGRGSLSPPLIMCTAATPMPTPKSSPFLDAAPSLPDSRRGSSSSGDPPLGDQKPPASLRSSPCAPWGPSGAWSSRRSSWSSLGRAPSLKRRGQCGERESLLSGEGKGSTDDEAEDGRAAPGPRATPLRRAESLDPRPLRPAALPPTKCRDRDGQVVALPSDFFLRIDSHREDAAELDDDSEDSCCLRLHKVLEPYKPQWCRSREAWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQCRAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRSTFPSPEAQRRPYYADYSPTRRSIHSLCTSHYLDLFITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRECSREDKHCLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAELDAEIELEMAQGPGSARRVDADRPPLPQESPGARDAPNLVARKVSVSRMLSLPNDSYMFRPVVPASAPHPRPLQEVEMETYGAGTPLGSVASVHSPPAESCASLQIPLAVSSPARSGEPLHALSPRGTARSPSLSRLLCRQEAVHTDSLEGKIDSPRDTLDPAEPGEKTPVRPVTQGGSLQSPPRSPRPASVRTRKHTFGQRCVSSRPAAPGGEEAEASDPADEEVSHITSSACPWQPTAEPHGPEASPVAGGERDLRRLYSVDAQGFLDKPGRADEQWRPSAELGSGEPGEAKAWGPEAEPALGARRKKKMSPPCISVEPPAEDEGSARPSAAEGGSTTLRRRTPSCEATPHRDSLEPTEGSGAGGDPAAKGERWGQASCRAEHLTVPSFAFEPLDLGVPSGDPFLDGSHSVTPESRASSSGAIVPLEPPESEPPMPVGDPPEKRRGLYLTVPQCPLEKPGSPSATPAPGGGADDPV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95180 基板として

Site PTM Type Enzyme
S215 Phosphorylation
T446 Phosphorylation
S561 Phosphorylation
T615 Phosphorylation
S619 Phosphorylation
S623 Phosphorylation
T749 Phosphorylation
S783 Phosphorylation
K1044 Ubiquitination
S1073 Phosphorylation
S1107 Phosphorylation P17612 (PRKACA)
S1144 Phosphorylation P17612 (PRKACA)
T1191 Phosphorylation
S1198 Phosphorylation
S1587 Phosphorylation
S1905 Phosphorylation
S1926 Phosphorylation
S1991 Phosphorylation
S1999 Phosphorylation
S2057 Phosphorylation
T2073 Phosphorylation
S2137 Phosphorylation
S2188 Phosphorylation

研究背景

機能:

Voltage-sensitive calcium channel that gives rise to T-type calcium currents. T-type calcium channels belong to the 'low-voltage activated (LVA)' group. A particularity of this type of channel is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle (Probable). They may also be involved in the modulation of firing patterns of neurons. In the adrenal zona glomerulosa, participates in the signaling pathway leading to aldosterone production in response to either AGT/angiotensin II, or hyperkalemia.

PTMs:

In response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.
Note: Interaction with STAC increases expression at the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

In nonneuronal tissues, the highest expression levels are found in the kidney, liver, and heart. In the brain, most abundant in the amygdala, caudate nucleus, and putamen. In the heart, expressed in blood vessels. Isoform 1 and isoform 2 are expressed in testis, primarily in the germ cells, but not in other portions of the reproductive tract, such as ductus deferens. Isoform 2 is not detected in brain. Expressed in the adrenal glomerulosa (at protein level).

サブユニット構造:

Interacts (via N-terminal cytoplasmic domain) with STAC.

タンパク質ファミリー:

Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1H subfamily.

研究領域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

参考文献

1). miR‐137 and its target T‐type CaV3.1 channel modulate dedifferentiation and proliferation of cerebrovascular smooth muscle cells in simulated microgravity rats by regulating calcineurin/NFAT pathway. CELL PROLIFERATION, 2020 (PubMed: 32034930) [IF=8.5]

Application: WB    Species: Rat    Sample: VSMCs

Figure 4 Comparisons of protein expression and whole‐cell current densities of T‐type VDCCs in cerebral VSMCs isolated from CON and SUS rats. A‐B, The protein expressions of T‐type CaV3.1 and CaV3.2 channels were examined by Western blotting. GAPDH was used as loading control (n = 10/group). C, mRNA levels of T‐type CaV3.1 channels were assessed by qRT‐PCR analysis. GAPDH was used for normalization (n = 10/group). D, Inward whole‐cell currents were recorded from isolated cerebral VSMCs at −80 mV and −40 mV holding potentials, respectively. E, Whole‐cell currents recorded from cerebral VSMCs with the extracellular application of 0.1 μmol/L Nifedipine and 5 μmol/L Mibefradil in the presence of 0.1 μmol/L Nifedipine. Cumulative I‐V curves are shown in the right panel. F, Whole‐cell currents recorded at −80 mV holding potential in the presence of 0.1 μmol/L Nifedipine were used as the representative T‐type CaV3.1 channels currents. The values are expressed as means ± SEM with the number of cells recorded in parentheses. Data were presented as mean ± SEM. *P < .05 vs control or Baseline, # P < .05 vs 0.1 μmol/L Nifedipine (analysed by Student's t test in B, C, D and F; analysed by ANOVA in E)

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