WIPF1 Antibody - #DF4616
| 製品: | WIPF1 Antibody |
| カタログ: | DF4616 |
| タンパク質の説明: | Rabbit polyclonal antibody to WIPF1 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 52 KD; 51kD(Calculated). |
| ユニプロット: | O43516 |
| RRID: | AB_2836980 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4616, RRID:AB_2836980.
折りたたみ／展開
AI115543; D2Ertd120e; MGC111041; Protein PRPL-2; PRPL 2; PRPL-2 protein; WAS/WASL interacting protein family member 1; WAS/WASL-interacting protein family member 1; WAS2; WASP-interacting protein; WASPIP; WIP; Wipf1; WIPF1_HUMAN; Wiskott-Aldrich syndrome protein interacting protein; Wiskott-Aldrich syndrome protein-interacting protein;
免疫原
Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.
- O43516 WIPF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGAGGGGGGFGGGGGFGGGGGGGGGGSFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPLLPPGGRSTSAKPFSPPSGPGRFPVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSPHNRGSPPVPGGPRQPSPGPTPPPFPGNRGTALGGGSIRQSPLSSSSPFSNRPPLPPTPSRALDDKPPPPPPPVGNRPSIHREAVPPPPPQNNKPPVPSTPRPSASSQAPPPPPPPSRPGPPPLPPSSSGNDETPRLPQRNLSLSSSTPPLPSPGRSGPLPPPPSERPPPPVRDPPGRSGPLPPPPPVSRNGSTSRALPATPQLPSRSGVDSPRSGPRPPLPPDRPSAGAPPPPPPSTSIRNGFQDSPCEDEWESRFYFHPISDLPPPEPYVQTTKSYPSKLARNESRSGSNRRERGAPPLPPIPR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43516 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R33 | Methylation | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| R125 | Methylation | Uniprot | |
| R134 | Methylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| R149 | Methylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| R211 | Methylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| R226 | Methylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| R236 | Methylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| R249 | Methylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| R258 | Methylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T398 | Phosphorylation | Uniprot | |
| R404 | Methylation | Uniprot | |
| R415 | Methylation | Uniprot | |
| R422 | Methylation | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| Y455 | Phosphorylation | Uniprot | |
| Y468 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Q04759 (PRKCQ) | Uniprot |
| R493 | Methylation | Uniprot |
研究背景
Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation. Plays a role in the formation of cell ruffles (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.
Cytoplasmic vesicle. Cytoplasm>Cytoskeleton. Cell projection>Ruffle.
Note: Vesicle surfaces and along actin tails. Colocalizes with actin stress fibers. When coexpressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL (By similarity).
Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.
Binds to WAS, profilin and actin. Binds to WASL (By similarity). Interacts with DBNL. Interacts with FNBP1L (via the SH3 domain).
Binds to WAS within the N-terminal region 170, at a site distinct from the CDC42-binding site.
Belongs to the verprolin family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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