WEE2 Antibody - #DF4617
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4617, RRID:AB_2836981.
折りたたみ／展開
hCG_39453; WEE1 homolog 2 (S. pombe); Wee1-like protein kinase 1B; Wee1-like protein kinase 2; WEE1B; Wee1B kinase; Wee2; WEE2_HUMAN;
免疫原
Expressed in oocytes (at protein level) (PubMed:29606300). May also be expressed in testis (PubMed:11029659).
- P0C1S8 WEE2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDKDIDKELRQKLNFSYCEETEIEGQKKVEESREASSQTPEKGEVQDSEAKGTPPWTPLSNVHELDTSSEKDKESPDQILRTPVSHPLKCPETPAQPDSRSKLLPSDSPSTPKTMLSRLVISPTGKLPSRGPKHLKLTPAPLKDEMTSLALVNINPFTPESYKKLFLQSGGKRKIRGDLEEAGPEEGKGGLPAKRCVLRETNMASRYEKEFLEVEKIGVGEFGTVYKCIKRLDGCVYAIKRSMKTFTELSNENSALHEVYAHAVLGHHPHVVRYYSSWAEDDHMIIQNEYCNGGSLQAAISENTKSGNHFEEPKLKDILLQISLGLNYIHNSSMVHLDIKPSNIFICHKMQSESSGVIEEVENEADWFLSANVMYKIGDLGHATSINKPKVEEGDSRFLANEILQEDYRHLPKADIFALGLTIAVAAGAESLPTNGAAWHHIRKGNFPDVPQELSESFSSLLKNMIQPDAEQRPSAAALARNTVLRPSLGKTEELQQQLNLEKFKTATLERELREAQQAQSPQGYTHHGDTGVSGTHTGSRSTKRLVGGKSARSSSFTSGEREPLH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P0C1S8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T83 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| T225 | Phosphorylation | Uniprot | |
| K389 | Acetylation | Uniprot | |
| T509 | Phosphorylation | Uniprot |
研究背景
Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1/CDC2 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).
Phosphorylated on serine residues. Phosphorylation leads to increase its activity (By similarity).
Nucleus.
Expressed in oocytes (at protein level). May also be expressed in testis.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
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