WASF2 Antibody - #DF4618
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4618, RRID:AB_2836982.
折りたたみ／展開
DICTYOSTELIUM; dJ393P12.2; IMD2; Protein WAVE-2; Putative Wiskott Aldrich syndrome protein family member 4; SCAR 2; SCAR; SCAR2; Suppressor of cyclic-AMP receptor (WASP family); Verprolin homology domain-containing protein 2; WASF2; WASF2_HUMAN; WASF4; WASP family protein member 2; WASP family protein member 4; WASP family Verprolin homologous protein 2; WASP-family protein member 2; WAVE 2; WAVE2; Wiskott-Aldrich syndrome protein family member 2; Wiskott-Aldrich syndrome protein family verprolin-homologous protein;
免疫原
Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.
- Q9Y6W5 WASF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSKYAEDIFGELFTQANTFASRVSSLAERVDRLQVKVTQLDPKEEEVSLQGINTRKAFRSSTIQDQKLFDRNSLPVPVLETYNTCDTPPPLNNLTPYRDDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKLGTSGYPPTLVYQNGSIGCVENVDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPVDNQRGSGLAGPKRSSVVSPSHPPPAPPLGSPPGPKPGFAPPPAPPPPPPPMIGIPPPPPPVGFGSPGTPPPPSPPSFPPHPDFAAPPPPPPPPAADYPTLPPPPLSQPTGGAPPPPPPPPPPGPPPPPFTGADGQPAIPPPLSDTTKPKSSLPAVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEFDEDDWSD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y6W5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T17 | Phosphorylation | Uniprot | |
| T80 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| T137 | Phosphorylation | Uniprot | |
| Y139 | Phosphorylation | Uniprot | |
| Y150 | Phosphorylation | A0A173G4P4 (Abl fusion) , P00519 (ABL1) | Uniprot |
| Y155 | Phosphorylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| Y230 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S298 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S343 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| T346 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S351 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S429 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | Uniprot | |
| T471 | Phosphorylation | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S482 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S484 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S488 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S489 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S497 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
研究背景
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium.
Note: At the interface between the lamellipodial actin meshwork and the membrane.
Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.
Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 (NCKAP1l/HEM1 in hematopoietic cells) and WASF2/WAVE2. Directly interacts with BRK1. Interacts with FNBP1L (via the SH3 domain).
(Microbial infection) Interacts with human cytomegalovirus protein UL135.
Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain.
Belongs to the SCAR/WAVE family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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