EME1 Antibody - #DF4671
| 製品: | EME1 Antibody |
| カタログ: | DF4671 |
| タンパク質の説明: | Rabbit polyclonal antibody to EME1 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 予測: | Rabbit, Dog |
| 分子量: | 65 KD; 63kD(Calculated). |
| ユニプロット: | Q96AY2 |
| RRID: | AB_2837022 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4671, RRID:AB_2837022.
折りたたみ／展開
6820428D13; Crossover junction endonuclease EME1; EC 3.1.22.-; EME1; Eme1 essential meiotic endonuclease 1 homolog 1 (S. pombe); EME1, S. pombe, homolog of, 1; EME1_HUMAN; essential meiotic endonuclease 1 homolog 1 (S pombe); Essential Meiotic Endonuclease 1 Homolog 1; essential meiotic endonuclease 1 homolog 2; Essential meiotic endonuclease 1, S. pombe, homolog of, 1; FLJ31364; hMMS4; homolog of yeast EME1 endonuclease; MGC106543; MMS4; MMS4 homolog; MMS4L;
免疫原
- Q96AY2 EME1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFPKIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTKPSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96AY2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| T33 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| K103 | Sumoylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| K136 | Sumoylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| K232 | Sumoylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| T238 | Phosphorylation | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| K338 | Methylation | Uniprot | |
| K338 | Sumoylation | Uniprot | |
| K340 | Methylation | Uniprot | |
| K340 | Ubiquitination | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| K441 | Ubiquitination | Uniprot | |
| K449 | Ubiquitination | Uniprot |
研究背景
Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
Nucleus>Nucleolus.
Note: Recruited to regions of DNA damage in S-phase cells.
May self-associate. Interacts with MUS81.
Belongs to the EME1/MMS4 family.
研究領域
· Genetic Information Processing > Replication and repair > Homologous recombination.
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
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