ELF4 Antibody - #DF4678
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4678, RRID:AB_2837029.
折りたたみ／展開
E74-like factor 4; ELF4; ELF4_HUMAN; ELFR; ETS-related transcription factor Elf-4; ETS-related transcription factor Elf4; MEF; Myeloid Elf-1-like factor; Myeloid Elf1-like factor;
免疫原
Abundantly expressed in the placenta and in a variety of myeloid leukemia cell lines. Moderate levels of expression in heart, lung, spleen, thymus, peripheral blood lymphocytes, ovary and colon. Lower levels of expression in Jurkat T-cells and other T-cell lines and no expression in brain.
- Q99607 ELF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAITLQPSDLIFEFASNGMDDDIHQLEDPSVFPAVIVEQVPYPDLLHLYSGLELDDVHNGIITDGTLCMTQDQILEGSFLLTDDNEATSHTMSTAEVLLNMESPSDILDEKQIFSTSEMLPDSDPAPAVTLPNYLFPASEPDALNRAGDTSDQEGHSLEEKASREESAKKTGKSKKRIRKTKGNRSTSPVTDPSIPIRKKSKDGKGSTIYLWEFLLALLQDRNTCPKYIKWTQREKGIFKLVDSKAVSKLWGKQKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKEMPKDLVVIEDEDESSEATAAPPQASTASVASASTTRRTSSRVSSRSAPQGKGSSSWEKPKIQHVGLQPSASLELGPSLDEEIPTTSTMLVSPAEGQVKLTKAVSASSVPSNIHLGVAPVGSGSALTLQTIPLTTVLTNGPPASTTAPTQLVLQSVPAASTFKDTFTLQASFPLNASFQDSQVAAPGAPLILSGLPQLLAGANRPTNPAPPTVTGAGPAGPSSQPPGTVIAAFIRTSGTTAAPRVKEGPLRSSSYVQGMVTGAPMEGLLVPEETLRELLRDQAHLQPLPTQVVSRGSHNPSLLGNQTLSPPSRPTVGLTPVAELELSSGSGSLLMAEPSVTTSGSLLTRSPTPAPFSPFNPTSLIKMEPHDI
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99607 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K111 | Sumoylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| Y262 | Phosphorylation | Uniprot | |
| R270 | Methylation | Uniprot | |
| Y272 | Phosphorylation | Uniprot | |
| Y273 | Phosphorylation | Uniprot | |
| K280 | Sumoylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| T527 | Phosphorylation | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| S641 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| T643 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S648 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K657 | Sumoylation | Uniprot |
研究背景
Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2 (By similarity). Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway.
Nucleus>PML body.
Note: Accumulation into PML nuclear bodies is mediated by PML.
Abundantly expressed in the placenta and in a variety of myeloid leukemia cell lines. Moderate levels of expression in heart, lung, spleen, thymus, peripheral blood lymphocytes, ovary and colon. Lower levels of expression in Jurkat T-cells and other T-cell lines and no expression in brain.
Interacts with RUNX1 (via the Runt domain); the interaction transactivates the IL3 promoter. Interacts (via its C-terminus) with PML; the interaction translocates ELF4 to PML nuclear bodies and enhances transactivation of LYZ.
Belongs to the ETS family.
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