SIRT3 Antibody - #DF4684
| 製品: | SIRT3 Antibody |
| カタログ: | DF4684 |
| タンパク質の説明: | Rabbit polyclonal antibody to SIRT3 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 分子量: | 28kD, 45KD; 44kD(Calculated). |
| ユニプロット: | Q9NTG7 |
| RRID: | AB_2837035 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4684, RRID:AB_2837035.
折りたたみ／展開
hSIRT 3; hSIRT3; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase; NAD dependent deacetylase sirtuin 3 mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; Regulatory protein SIR2 homolog 3; Silent mating type information regulation 2 S.cerevisiae homolog 3; Sir 2 like 3; SIR 2 like protein 3; SIR 3; SIR2 L3; Sir2 like 3; SIR2 like protein 3; SIR2-like protein 3; SIR2L3; SIR3_HUMAN; SIRT 3; SIRT3; Sirtuin 3; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae); Sirtuin type 3; Sirtuin3;
免疫原
- Q9NTG7 SIR3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRDDVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK
PTMs - Q9NTG7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R51 | Methylation | Uniprot | |
| R57 | Methylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| T150 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S159 | Phosphorylation | P06493 (CDK1) | Uniprot |
| R276 | Methylation | Uniprot | |
| T284 | Phosphorylation | Uniprot | |
| R356 | Methylation | Uniprot | |
| T391 | Phosphorylation | Uniprot |
研究背景
NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity).
Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
Mitochondrion matrix.
Widely expressed.
Upon metabolic stress, forms a complex composed of FOXO3, SIRT3 and mitochondrial RNA polymerase POLRMT; the complex is recruited to mtDNA in a SIRT3-dependent manner. Also forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT. Interacts with NDUFA9, ACSS1, IDH2 and GDH. Interacts with PCCA.
Belongs to the sirtuin family. Class I subfamily.
研究領域
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
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