GNE Antibody - #DF4690
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4690, RRID:AB_2837041.
折りたたみ／展開
2310066H07Rik; Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase; DMRV; GLCNE_HUMAN; Glucosamine (UDP N acetyl) 2 epimerase/N acetylmannosamine kinase; GNE; IBM2; ManAc kinase; N acylmannosamine kinase; N-acetylmannosamine kinase; NM; RP23-209M8.6; Uae1; UDP GlcNAc 2 epimerase; UDP GlcNAc 2 epimerase/ManAc kinase; UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase; UDP-GlcNAc-2-epimerase; UDP-GlcNAc-2-epimerase/ManAc kinase; Uridine diphosphate N acetylglucosamine 2 epimerase; Uridine diphosphate-N-acetylglucosamine-2-epimerase;
免疫原
Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon.
- Q9Y223 GLCNE_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y223 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y22 | Phosphorylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K193 | Acetylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| K602 | Ubiquitination | Uniprot | |
| K618 | Ubiquitination | Uniprot | |
| K632 | Ubiquitination | Uniprot | |
| K637 | Ubiquitination | Uniprot |
研究背景
Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.
Phosphorylated by PKC.
Cytoplasm.
Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon.
Homodimer and homohexamer.
In the N-terminal section; belongs to the UDP-N-acetylglucosamine 2-epimerase family.
In the C-terminal section; belongs to the ROK (NagC/XylR) family.
研究領域
· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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