DUSP10 Antibody - #DF4694

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製品: DUSP10 Antibody
カタログ: DF4694
タンパク質の説明: Rabbit polyclonal antibody to DUSP10
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 52 KD; 53kD(Calculated).
ユニプロット: Q9Y6W6
RRID: AB_2837045

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
DUSP10 Antibody detects endogenous levels of total DUSP10.
RRID:
AB_2837045
引用形式: Affinity Biosciences Cat# DF4694, RRID:AB_2837045.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Dual specificity phosphatase 10; Dual specificity phosphatase MKP 5; Dual specificity phosphatase MKP5; Dual specificity protein phosphatase 10; DUS10_HUMAN; DUSP 10; DUSP10; DUSP10 protein; MAP kinase phosphatase 5; Mitogen activated protein kinase phosphatase 5; Mitogen-activated protein kinase phosphatase 5; MKP 5; MKP-5; MKP5; Serine/threonine specific protein phosphatase;

免疫原

免疫原:
Uniprot:

Q9Y6W6(DUS10_HUMAN) >>Visit HPA database.

遺伝子(ID):
DUSP10(11221)
発現特異性:
Q9Y6W6 DUS10_HUMAN:

Expressed in keratinocytes (at protein level) (PubMed:29043977). Detected in brain (PubMed:16806267).

タンパク質配列:
MPPSPLDDRVVVALSRPVRPQDLNLCLDSSYLGSANPGSNSHPPVIATTVVSLKAANLTYMPSSSGSARSLNCGCSSASCCTVATYDKDNQAQTQAIAAGTTTTAIGTSTTCPANQMVNNNENTGSLSPSSGVGSPVSGTPKQLASIKIIYPNDLAKKMTKCSKSHLPSQGPVIIDCRPFMEYNKSHIQGAVHINCADKISRRRLQQGKITVLDLISCREGKDSFKRIFSKEIIVYDENTNEPSRVMPSQPLHIVLESLKREGKEPLVLKGGLSSFKQNHENLCDNSLQLQECREVGGGASAASSLLPQPIPTTPDIENAELTPILPFLFLGNEQDAQDLDTMQRLNIGYVINVTTHLPLYHYEKGLFNYKRLPATDSNKQNLRQYFEEAFEFIEEAHQCGKGLLIHCQAGVSRSATIVIAYLMKHTRMTMTDAYKFVKGKRPIISPNLNFMGQLLEFEEDLNNGVTPRILTPKLMGVETVV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6W6 基板として

Site PTM Type Enzyme
S4 Phosphorylation
S224 Phosphorylation P42345 (MTOR)
S230 Phosphorylation P42345 (MTOR)
Y236 Phosphorylation
T240 Phosphorylation
K380 Ubiquitination

研究背景

機能:

Protein phosphatase involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily. It preferably dephosphorylates p38.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in keratinocytes (at protein level). Detected in brain.

サブユニット構造:

Monomer. Interacts with MAPK14.

タンパク質ファミリー:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

研究領域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

参考文献

1). Oroxylin A alleviates myocardial ischemia-reperfusion injury by quelling ferroptosis via activating the DUSP10/MAPK-Nrf2 pathway. Phytotherapy research : PTR, 2024 (PubMed: 39225191) [IF=6.1]
2). DUSP10 is a novel immune-related biomarker connected with survival and cellular proliferation in lower-grade glioma. Aging (Albany NY), 2023 (PubMed: 37387540) [IF=3.9]

Application: WB    Species: Human    Sample: LGG tissues

Figure 7 In vitro experiments verification of DUSP10 in LGG. (A) Western blot analysis of DUSP10 expression in LGG tissues and corresponding para-carcinoma tissues. (B) Western blot and (C) qRT-PCR analysis of DUSP10 expression in NHA and LGG cell lines. (D) The cell viability of si-DUSP10-transfected and si-NC-transfected SW1088 cells by CCK-8 assays. (E, F) Effect of DUSP10 knockdown on colony formation was counted in SW1088 cells. (G, H) Representative images (G) and histogram analysis (H) of EdU assays after DUSP10 knockdown in SW1088 cells. (I, J) Cell cycle assays were implemented to evaluate the cell distribution of the SW1088 cell lines transfected with si-DUSP10 or si-NC lentiviruses.

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