PTGIS Antibody - #DF4745

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製品: PTGIS Antibody
カタログ: DF4745
タンパク質の説明: Rabbit polyclonal antibody to PTGIS
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 57 KD; 57kD(Calculated).
ユニプロット: Q16647
RRID: AB_2837096

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(82%), Bovine(100%), Horse(91%), Sheep(100%), Dog(91%)
クローナリティ:
Polyclonal
特異性:
PTGIS Antibody detects endogenous levels of total PTGIS.
RRID:
AB_2837096
引用形式: Affinity Biosciences Cat# DF4745, RRID:AB_2837096.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CYP8; CYP8A1; Cytochrome P450, family 8, subfamily A, polypeptide 1; EC 5.3.99.4; MGC126858; MGC126860; OTTHUMP00000031777; PGIS; Prostacyclin synthase; Prostaglandin I2 (prostacyclin) synthase; Prostaglandin I2 synthase; PTGI; PTGIS; PTGIS_HUMAN;

免疫原

免疫原:
Uniprot:

Q16647(PTGIS_HUMAN) >>Visit HPA database.

遺伝子(ID):
PTGIS(5740)
発現特異性:
Q16647 PTGIS_HUMAN:

Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate.

タンパク質配列:
MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMKEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Horse
91
Dog
91
Pig
82
Zebrafish
64
Xenopus
56
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16647 基板として

Site PTM Type Enzyme
S52 Phosphorylation
S118 Phosphorylation
S200 Phosphorylation
Y398 Phosphorylation
T399 Phosphorylation

研究背景

機能:

Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).

細胞の位置付け:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate.

タンパク質ファミリー:

Belongs to the cytochrome P450 family.

研究領域

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). Vitamin D stimulates miR-26b-5p to inhibit placental COX-2 expression in preeclampsia. Scientific Reports, 2021 (PubMed: 34045549) [IF=4.6]

Application: IHC    Species: human    Sample: placental

Supplementary Figure 1|. Representative PGIS expression in placenta from normotensive pregnant women and women complicated with preeclampsia (PE). PGIS, prostacyclin synthase, Bar = 100 micron.
2). Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34007408)

Application: WB    Species: Rat    Sample: prostate cell

Figure 6 KLK1 could upregulate NO/cGMP signal and inhibit fibrosis in the prostate of aged rats. (a, b, e, and h) NO content, cGMP content, ADMA content, and cAMP content normalized to total protein concentration of rat prostate samples. (c, f, and i) Representative Western blot results of eNOS, DDAH2, COX-2, PTGIS, TGF-β1, RhoA, and ROCK1 in prostates of all three groups. (d, g, and j) The expression levels of above-mentioned proteins with β-actin as the loading control in all three groups. For each group, values are presented as the mean ± SD of 10 rats per group. ∗P < 0.05, ∗∗P < 0.01 (aTGR vs. aWTR). ##P < 0.01 (aWTR vs. yWTR).

Application: WB    Species: rat    Sample: prostate

Figure 6|: KLK1 could upregulate NO/cGMP signal and inhibit fibrosis in the prostate of aged rats.(c, f, and i) Representative Western blot results of eNOS, DDAH2, COX-2, PTGIS, TGF-β1, RhoA, and ROCK1 in prostates of all three groups.

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