CD168 Antibody - #DF4809

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製品: CD168 Antibody
カタログ: DF4809
タンパク質の説明: Rabbit polyclonal antibody to CD168
アプリケーション: WB IHC IF/ICC
反応性: Human
予測: Pig, Horse, Dog
分子量: 85 KD; 84kD(Calculated).
ユニプロット: O75330
RRID: AB_2837160

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(80%), Horse(80%), Dog(80%)
クローナリティ:
Polyclonal
特異性:
CD168 Antibody detects endogenous levels of total CD168.
RRID:
AB_2837160
引用形式: Affinity Biosciences Cat# DF4809, RRID:AB_2837160.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CD168; CD168 antigen; HMMR; HMMR_HUMAN; Hyaluronan mediated motility receptor; Hyaluronan-mediated motility receptor (RHAMM); IHABP; Intracellular hyaluronic acid-binding protein; MGC119494; MGC119495; OTTHUMP00000196920; Receptor for hyaluronan-mediated motility; RHAMM;

免疫原

免疫原:
Uniprot:

O75330(HMMR_HUMAN) >>Visit HPA database.

遺伝子(ID):
HMMR(3161)
発現特異性:
O75330 HMMR_HUMAN:

Expressed in breast cancer cell lines and in normal breast tissue.

タンパク質配列:
MSFPKAPLKRFNDPSGCAPSPGAYDVKTLEVLKGPVSFQKSQRFKQQKESKQNLNVDKDTTLPASARKVKSSESKESQKNDKDLKILEKEIRVLLQERGAQDRRIQDLETELEKMEARLNAALREKTSLSANNATLEKQLIELTRTNELLKSKFSENGNQKNLRILSLELMKLRNKRETKMRGMMAKQEGMEMKLQVTQRSLEESQGKIAQLEGKLVSIEKEKIDEKSETEKLLEYIEEISCASDQVEKYKLDIAQLEENLKEKNDEILSLKQSLEENIVILSKQVEDLNVKCQLLEKEKEDHVNRNREHNENLNAEMQNLKQKFILEQQEREKLQQKELQIDSLLQQEKELSSSLHQKLCSFQEEMVKEKNLFEEELKQTLDELDKLQQKEEQAERLVKQLEEEAKSRAEELKLLEEKLKGKEAELEKSSAAHTQATLLLQEKYDSMVQSLEDVTAQFESYKALTASEIEDLKLENSSLQEKAAKAGKNAEDVQHQILATESSNQEYVRMLLDLQTKSALKETEIKEITVSFLQKITDLQNQLKQQEEDFRKQLEDEEGRKAEKENTTAELTEEINKWRLLYEELYNKTKPFQLQLDAFEVEKQALLNEHGAAQEQLNKIRDSYAKLLGHQNLKQKIKHVVKLKDENSQLKSEVSKLRCQLAKKKQSETKLQEELNKVLGIKHFDPSKAFHHESKENFALKTPLKEGNTNCYRAPMECQESWK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
80
Horse
80
Dog
80
Bovine
78
Sheep
67
Xenopus
60
Rabbit
60
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75330 基板として

Site PTM Type Enzyme
S2 Phosphorylation
S20 Phosphorylation
S41 Phosphorylation
S65 Phosphorylation
T110 Phosphorylation
K114 Ubiquitination
K138 Ubiquitination
T198 Phosphorylation
S205 Phosphorylation
K215 Ubiquitination
K221 Ubiquitination
Y250 Phosphorylation
K251 Ubiquitination
K338 Ubiquitination
K350 Ubiquitination
S353 Phosphorylation
K419 Ubiquitination
K489 Ubiquitination
K604 Ubiquitination
K635 Ubiquitination
S656 Phosphorylation
K696 Ubiquitination
T703 Phosphorylation
Y713 Phosphorylation

研究背景

機能:

Receptor for hyaluronic acid (HA) (By similarity). Involved in cell motility (By similarity). When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenisis (By similarity).

細胞の位置付け:

Cell surface. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in breast cancer cell lines and in normal breast tissue.

サブユニット構造:

Subunit of the HARC complex. Interacts with ANKRD26.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

参考文献

1). Coupled scRNA-seq and Bulk-seq reveal the role of HMMR in hepatocellular carcinoma. Frontiers in immunology, 2024 (PubMed: 38633247) [IF=7.3]

Application: WB    Species: Human    Sample: HCC cells

Figure 3 Knockdown of HMMR inhibited proliferation and invasion of HCC cells. (A) The knockdown efficiency of si-HMMRs have been testified by western blot in SMMC-7721, Huh7 and LM3. (B, C) The results of clone assays. (D, E) The results of Edu assays. (F, G) The results of wound healing assays.**P
2). The critical role of B4GALT4 in promoting microtubule spindle assembly in HCC through the regulation of PLK1 and RHAMM expression. JOURNAL OF CELLULAR PHYSIOLOGY, 2022 (PubMed: 34270095) [IF=5.6]

Application: WB    Species: Human    Sample: HepG2 cells

FIGURE 9 B4GALT4 knockdown regulated TGF‐beta1/TGF‐beta receptor‐1 signaling pathway and modified the expressions of PLK1 and RHAMM. (a) B4GALT4 knockdown downregulated the expression of lumican. (b) The expressions of B4GALT4, P‐Smad2, Smad2, PLK1, and RHAMM in HepG2 cells after different treatments as mentioned above were detected by Western blot analysis. (c) The comparison of the mRNA expression level of HMMR between tumor and normal samples. (d) Survival analysis of HMMR in HCC. (e) The protein interaction network diagram of RHAMM, PLK1, and other pathway‐related genes. (f) ChIP‐seq results showed that Smad2/3 and p300 binding regions are enriched around the promoter of PLK1. (g) Schematic illustration of the B4GALT4‐mediated regulatory network responsible for the microtubule spindle assembly in HCC (****p<0.0001). B4GALT, beta 1,4‐galactosyltransferase; HCC, hepatocellular carcinoma; mRNA, messenger RNA

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