TOLLIP Antibody - #DF4836
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4836, RRID:AB_2837201.
折りたたみ／展開
Adapter protein; FLJ33531; IL 1RAcPIP; IL1RAcPIP; TOLIP; TOLIP_HUMAN; Toll interacting protein; Toll-interacting protein; tollip;
免疫原
- Q9H0E2 TOLIP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H0E2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| Y13 | Phosphorylation | Uniprot | |
| Y45 | Phosphorylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| Y68 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| Y76 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| K235 | Acetylation | Uniprot | |
| K235 | Ubiquitination | Uniprot |
研究背景
Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates.
Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products.
Cytoplasm.
Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2. Interacts with ATG8 family proteins (via AIM motifs), and ubiquitin (via CUE domain).
Both ATG8-interaction motifs (AIM1 and AIM2) are required for the association with ATG8 family proteins.
Belongs to the tollip family.
研究領域
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
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