ADORA1 Antibody - #DF4905

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製品: ADORA1 Antibody
カタログ: DF4905
タンパク質の説明: Rabbit polyclonal antibody to ADORA1
アプリケーション: WB
反応性: Human, Rat
予測: Pig, Horse, Rabbit
分子量: 37 KD; 37kD(Calculated).
ユニプロット: P30542
RRID: AB_2837258

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(83%), Horse(92%), Rabbit(89%)
クローナリティ:
Polyclonal
特異性:
ADORA1 Antibody detects endogenous levels of total ADORA1.
RRID:
AB_2837258
引用形式: Affinity Biosciences Cat# DF4905, RRID:AB_2837258.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

A1AR; A1R; AA1R; AA1R_HUMAN; Adenosine receptor A1; ADORA 1; ADORA 1; ADORA1; RDC 7; RDC7; Ri;

免疫原

免疫原:
Uniprot:

P30542(AA1R_HUMAN) >>Visit HPA database.

遺伝子(ID):
ADORA1(134)
タンパク質配列:
MPPSISAFQAAYIGIEVLIALVSVPGNVLVIWAVKVNQALRDATFCFIVSLAVADVAVGALVIPLAILINIGPQTYFHTCLMVACPVLILTQSSILALLAIAVDRYLRVKIPLRYKMVVTPRRAAVAIAGCWILSFVVGLTPMFGWNNLSAVERAWAANGSMGEPVIKCEFEKVISMEYMVYFNFFVWVLPPLLLMVLIYLEVFYLIRKQLNKKVSASSGDPQKYYGKELKIAKSLALILFLFALSWLPLHILNCITLFCPSCHKPSILTYIAIFLTHGNSAMNPIVYAFRIQKFRVTFLKIWNDHFRCQPAPPIDEDLPEERPDD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Rabbit
89
Pig
83
Bovine
75
Sheep
75
Dog
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30542 基板として

Site PTM Type Enzyme
K214 Methylation
K224 Methylation

研究背景

機能:

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

研究領域

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

参考文献

1). Impact of Caffeine Consumption on Type 2 Diabetes-Induced Spatial Memory Impairment and Neurochemical Alterations in the Hippocampus. Frontiers in Neuroscience, 2019 (PubMed: 30686981) [IF=4.3]

Application: WB    Species: rat    Sample: hippocampus

Figure 6. |Density A1R and A2AR analysed by Western blot in nerve terminal-enriched membranes(A) and total membranes (B) from the hippocampus of GK and Wistar (W) rats, either receiving 1g/L caffeine (Caff) or tap water. Samples were loaded in SDS-PAGE gels at a protein concentration of 40 or 150 μg for A1R (n=6-8) and A2AR (n=2-3), respectively. Immunoreactivity was normalised to either α-tubulin or β-actin, and calculated as percentage of control in the same Western blot experiment. Symbols represent LSD test results after ANOVA with either significant diabetes effect or significant diabetes-caffeine interaction: *P<0.05, **P<0.01 for GK versus Wistar in the respective treatment group or as indicated.

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