SRC3 Antibody - #BF0072

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製品: SRC3 Antibody
カタログ: BF0072
タンパク質の説明: Mouse monoclonal antibody to SRC3
アプリケーション: WB ELISA
反応性: Human
分子量: 155kDa; 155kD(Calculated).
ユニプロット: Q9Y6Q9
RRID: AB_2833670

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製品説明

ソース:
Mouse
アプリケーション:
ELISA 1:10000, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFB1646]
特異性:
SRC3 antibody detects endogenous levels of total SRC3.
RRID:
AB_2833670
引用形式: Affinity Biosciences Cat# BF0072, RRID:AB_2833670.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ACTR; AIB 1; AIB-1; AIB1; Amplified in breast cancer 1; Amplified in breast cancer 1 protein; bHLHe42; CAGH 16; CAGH16; CBP interacting protein; CBP-interacting protein; Class E basic helix-loop-helix protein 42; CTG 26; CTG26; KAT13B / AIB1; MGC141848; NCOA 3; NCoA-3; Ncoa3; NCOA3_HUMAN; Nuclear receptor coactivator 3; pCIP; RAC 3; RAC-3; RAC3; Receptor associated coactivator 3; Receptor-associated coactivator 3; SRC 3; SRC-3; SRC3; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC14; TNRC16; TRAM 1; TRAM-1; TRAM1;

免疫原

免疫原:

Purified recombinant fragment of human SRC3 expressed in E. Coli.

Uniprot:

Q9Y6Q9(NCOA3_HUMAN) >>Visit HPA database.

遺伝子(ID):
NCOA3(8202)
発現特異性:
Q9Y6Q9 NCOA3_HUMAN:

Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.

タンパク質の説明:
NCOA3: nuclear receptor coactivator 3. The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation.
タンパク質配列:
MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQIRQIKEQGKTISNDDDVQKADVSSTGQGVIDKDSLGPLLLQALDGFLFVVNRDGNIVFVSENVTQYLQYKQEDLVNTSVYNILHEEDRKDFLKNLPKSTVNGVSWTNETQRQKSHTFNCRMLMKTPHDILEDINASPEMRQRYETMQCFALSQPRAMMEEGEDLQSCMICVARRITTGERTFPSNPESFITRHDLSGKVVNIDTNSLRSSMRPGFEDIIRRCIQRFFSLNDGQSWSQKRHYQEAYLNGHAETPVYRFSLADGTIVTAQTKSKLFRNPVTNDRHGFVSTHFLQREQNGYRPNPNPVGQGIRPPMAGCNSSVGGMSMSPNQGLQMPSSRAYGLADPSTTGQMSGARYGGSSNIASLTPGPGMQSPSSYQNNNYGLNMSSPPHGSPGLAPNQQNIMISPRNRGSPKIASHQFSPVAGVHSPMASSGNTGNHSFSSSSLSALQAISEGVGTSLLSTLSSPGPKLDNSPNMNITQPSKVSNQDSKSPLGFYCDQNPVESSMCQSNSRDHLSDKESKESSVEGAENQRGPLESKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLLQNGNSPAEVAKITAEATGKDTSSITSCGDGNVVKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSSDFYNNSISSNGSHLGTKQQVFQGTNSLGLKSSQSVQSIRPPYNRAVSLDSPVSVGSSPPVKNISAFPMLPKQPMLGGNPRMMDSQENYGSSMGGPNRNVTVTQTPSSGDWGLPNSKAGRMEPMNSNSMGRPGGDYNTSLPRPALGGSIPTLPLRSNSIPGARPVLQQQQQMLQMRPGEIPMGMGANPYGQAAASNQLGSWPDGMLSMEQVSHGTQNRPLLRNSLDDLVGPPSNLEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDAFQGQEAAVMMDQKAGLYGQTYPAQGPPMQGGFHLQGQSPSFNSMMNQMNQQGNFPLQGMHPRANIMRPRTNTPKQLRMQLQQRLQGQQFLNQSRQALELKMENPTAGGAAVMRPMMQPQVSSQQGFLNAQMVAQRSRELLSHHFRQQRVAMMMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTQAFSPPPNVTASPSMDGLLAGPTMPQAPPQQFPYQPNYGMGQQPDPAFGRVSSPPNAMMSSRMGPSQNPMMQHPQAASIYQSSEMKGWPSGNLARNSSFSQQQFAHQGNPAVYSMVHMNGSSGHMGQMNMNPMPMSGMPMGPDQKYC

PTMs - Q9Y6Q9 基板として

Site PTM Type Enzyme
S2 Acetylation
T24 Phosphorylation P45983 (MAPK8) , Q16539 (MAPK14)
T30 Phosphorylation
S32 Phosphorylation
K35 Acetylation
K65 Ubiquitination
K97 Ubiquitination
S101 Phosphorylation
S102 Phosphorylation P49674 (CSNK1E)
K167 Ubiquitination
T184 Phosphorylation
K191 Ubiquitination
S214 Phosphorylation
S244 Phosphorylation
R251 Methylation
S274 Phosphorylation
K276 Acetylation
T282 Phosphorylation
S284 Phosphorylation
S287 Phosphorylation
S288 Phosphorylation
S306 Phosphorylation
S505 Phosphorylation P45983 (MAPK8) , Q16539 (MAPK14)
S509 Phosphorylation
S543 Phosphorylation P45983 (MAPK8) , Q16539 (MAPK14)
S551 Phosphorylation
T557 Phosphorylation
S563 Phosphorylation
S569 Phosphorylation
Y574 Phosphorylation
S601 Phosphorylation P48730 (CSNK1D)
K616 Acetylation
K619 Acetylation
K620 Acetylation
K620 Ubiquitination
S636 Phosphorylation
S640 Phosphorylation
S664 Phosphorylation
K687 Acetylation
S694 Phosphorylation
T714 Phosphorylation
S715 Phosphorylation
K723 Sumoylation
S728 Phosphorylation P06493 (CDK1)
K753 Ubiquitination
K786 Sumoylation
S794 Phosphorylation
S836 Phosphorylation
K840 Methylation
R849 Methylation
S857 Phosphorylation O15111 (CHUK) , Q16659 (MAPK6) , P17612 (PRKACA)
S860 Phosphorylation Q16539 (MAPK14) , P45983 (MAPK8)
S863 Phosphorylation
S866 Phosphorylation
S867 Phosphorylation P06493 (CDK1) , Q16539 (MAPK14) , P45983 (MAPK8)
K926 Ubiquitination
R951 Methylation
S1033 Phosphorylation Q05513 (PRKCZ)
S1042 Phosphorylation Q05513 (PRKCZ)
S1048 Phosphorylation Q05513 (PRKCZ)
T1059 Phosphorylation Q05513 (PRKCZ)
S1062 Phosphorylation Q05513 (PRKCZ)
T1064 Phosphorylation Q05513 (PRKCZ)
T1067 Phosphorylation Q05513 (PRKCZ)
K1091 Methylation
T1114 Phosphorylation Q05513 (PRKCZ)
R1171 Methylation
R1177 Methylation
R1188 Methylation
K1194 Sumoylation
S1330 Phosphorylation
Y1357 Phosphorylation P00519 (ABL1)

研究背景

機能:

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.

PTMs:

Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.

Methylated by CARM1.

Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.

サブユニット構造:

Interacts with CARM1 (By similarity). Present in a complex containing NCOA2, IKKA, IKKB, IKBKG and the histone acetyltransferase protein CREBBP. Interacts with CASP8AP2, NR3C1 and PCAF. Interacts with ATAD2 and this interaction is enhanced by estradiol. Found in a complex containing NCOA3, AR and MAK. Interacts with DDX5. Interacts with PSMB9. Interacts with NPAS2. Interacts with NR4A3 (By similarity). Interacts with ESRRB; mediates the interaction between ESRRB and RNA polymerase II complexes and allows NCOA3 corecruitment to ESRRB, KLF4, NANOG, and SOX2 enhancer regions to trigger ESRRB-dependent gene activation involved in self-renewal and pluripotency (By similarity).

タンパク質ファミリー:

Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).

Belongs to the SRC/p160 nuclear receptor coactivator family.

研究領域

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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