TAS2R10 Antibody - #DF5157

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製品: TAS2R10 Antibody
カタログ: DF5157
タンパク質の説明: Rabbit polyclonal antibody to TAS2R10
アプリケーション: WB IF/ICC
反応性: Human
分子量: 35 KD; 35kD(Calculated).
ユニプロット: Q9NYW0
RRID: AB_2837347

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
TAS2R10 Antibody detects endogenous levels of total TAS2R10.
RRID:
AB_2837347
引用形式: Affinity Biosciences Cat# DF5157, RRID:AB_2837347.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

MGC126811; MGC126813; T2R10; T2R10_HUMAN; TAS2R10; Taste receptor family B member 2; Taste receptor type 2 member 10; Taste receptor, family B, member 2; Taste receptor, type 2, member 10; TRB2;

免疫原

免疫原:

A synthesized peptide derived from human TAS2R10, corresponding to a region within the internal amino acids.

Uniprot:

Q9NYW0(T2R10_HUMAN) >>Visit HPA database.

遺伝子(ID):
TAS2R10(50839)
発現特異性:
Q9NYW0 T2R10_HUMAN:

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.

タンパク質配列:
MLRVVEGIFIFVVVSESVFGVLGNGFIGLVNCIDCAKNKLSTIGFILTGLAISRIFLIWIIITDGFIQIFSPNIYASGNLIEYISYFWVIGNQSSMWFATSLSIFYFLKIANFSNYIFLWLKSRTNMVLPFMIVFLLISSLLNFAYIAKILNDYKTKNDTVWDLNMYKSEYFIKQILLNLGVIFFFTLSLITCIFLIISLWRHNRQMQSNVTGLRDSNTEAHVKAMKVLISFIILFILYFIGMAIEISCFTVRENKLLLMFGMTTTAIYPWGHSFILILGNSKLKQASLRVLQQLKCCEKRKNLRVT

PTMs - Q9NYW0 基板として

Site PTM Type Enzyme
T48 Phosphorylation
S53 Phosphorylation

研究背景

機能:

Gustducin-coupled strychnine receptor implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5.

細胞の位置付け:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor T2R family.

研究領域

· Organismal Systems > Sensory system > Taste transduction.

参考文献

1). Biomimetic in vitro respiratory system using smooth muscle cells on ECIS chips for anti-asthma TCMs screening. Analytica Chimica Acta, 2021 (PubMed: 33926702) [IF=6.2]

Application: IF/ICC    Species: Mouse    Sample: ASM cells

Fig. 2. Identification of TAS2Rs in mouse ASM cells. (A) RT-qPCR of mTAS2Rs expression in mouse ASM cells, with b-actin as internal control. Results were normalized to expression of the Adora1. Data are collected from three independent experiments. (B) Immunostaining with DAPI (blue), anti-TAS2R10, and anti-TAS2R14 receptor antibody (red), scale bar ¼ 20 mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

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