Neurotensin Receptor 1 Antibody - #DF5002

(1)  
製品: Neurotensin Receptor 1 Antibody
カタログ: DF5002
タンパク質の説明: Rabbit polyclonal antibody to Neurotensin Receptor 1
アプリケーション: WB IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep
分子量: 46 KD; 46kD(Calculated).
ユニプロット: P30989
RRID: AB_2837361

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説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(88%), Bovine(88%), Horse(88%), Sheep(88%)
クローナリティ:
Polyclonal
特異性:
Neurotensin Receptor 1 Antibody detects endogenous levels of total Neurotensin Receptor 1.
RRID:
AB_2837361
引用形式: Affinity Biosciences Cat# DF5002, RRID:AB_2837361.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

High affinity levocabastine insensitive neurotensin receptor; Neurotensin receptor 1 (high affinity); Neurotensin receptor type 1; NT R 1; NTR; NTR1; NTR1_HUMAN; NTRH; NTRR; NTSR 1;

免疫原

免疫原:
Uniprot:

P30989(NTR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
NTSR1(4923)
発現特異性:
P30989 NTR1_HUMAN:

Expressed in prostate (at protein level). Detected in colon and peripheral blood mononuclear cells. Detected at very low levels in brain.

タンパク質配列:
MRLNSSAPGTPGTPAADPFQRAQAGLEEALLAPGFGNASGNASERVLAAPSSELDVNTDIYSKVLVTAVYLALFVVGTVGNTVTAFTLARKKSLQSLQSTVHYHLGSLALSDLLTLLLAMPVELYNFIWVHHPWAFGDAGCRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLMSRSRTKKFISAIWLASALLAVPMLFTMGEQNRSADGQHAGGLVCTPTIHTATVKVVIQVNTFMSFIFPMVVISVLNTIIANKLTVMVRQAAEQGQVCTVGGEHSTFSMAIEPGRVQALRHGVRVLRAVVIAFVVCWLPYHVRRLMFCYISDEQWTPFLYDFYHYFYMVTNALFYVSSTINPILYNLVSANFRHIFLATLACLCPVWRRRRKRPAFSRKADSVSSNHTLSSNATRETLY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
75
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30989 基板として

Site PTM Type Enzyme
K398 Ubiquitination
S401 Phosphorylation
S403 Phosphorylation
S404 Phosphorylation
T413 Phosphorylation
Y418 Phosphorylation

研究背景

機能:

G-protein coupled receptor for the tridecapeptide neurotensin (NTS). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis.

PTMs:

N-glycosylated.

Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Membrane raft.
Note: Palmitoylation is required for localization at CAV1-enriched membrane rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in prostate (at protein level). Detected in colon and peripheral blood mononuclear cells. Detected at very low levels in brain.

サブユニット構造:

Interacts (palmitoylated form) with GNA11.

タンパク質ファミリー:

The ligand binding pocket consists mainly of extracellular loops ECL2 and ECL3, as well as transmembrane regions TM6 and TM7.

Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

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