RHO Antibody - #DF5046

Fig. 4: Mas inhibits VECs migration through RhoA/ROCK signaling pathway in VECs. A Representative images of wound healing assays. HUVECs had been exposed to different concentrations of Mas (0.3, 1, 3 μM) for 12 h. B Representative images of migrated HCAECs that had been exposed to different concentrations of Mas (0.3, 1, 3 μM). The migration capacity of HCAECs was determined using a Transwell culture system. C The quantification of migrated cells was counted from 5 different views. D Representative images of wound healing assays. HUVECs had been exposed to VEGF (1 nM), Mas (1 μM) + VEGF (1 nM) for 12 h. E Representative images of migrated HCAECs that had been exposed to VEGF (1 nM), Mas (1 μM) + VEGF (1 nM) for 12 h.The migration capacity of HCAECs was determined using a Transwell culture system. F The quantification of migrated cells was counted from 5 different views. Cells were observed at 40×magnification. G Representative immunofluorescence images for double-labeling of RhoA (yellow) and ROCK1 (red) expressions of aorta, DAPI (blue) (n = 5 per group). Scale bar: 50 μm (low magnification) and 20 μm (high magnification). H RhoA and ROCK1 fluorescence intensity. I–K Representative western blot analysis to determine the proteins expression of RhoA and ROCK1 in HUVEC cells. L In the absence or presence of 10 μM of ROCK inhibitor Y-27632, the migration capacity of HCAECs was determined using the Transwell culture system. M The quantification of migrated cells was counted from 5 different views. Cells were observed at 40×magnification. One-way ANOVA was followed by post hoc Tukey’s test. All results are presented as mean ± SEM. **P 

Fig. 1 Characterization of MSC photoreceptors for blue/red light irradiation. a Schema of the LED photosystem. b Real-time PCR screening of the basal and stimulated expression of opsins as indicated (OPN4 constitutional expression level was selected as the control group, fold-change = 1). c Proteins were extracted from P2, P4, and P6 passages of MSCs and subjected to immunoblotting. d Proteins were extracted from P4 passage of MSCs with blue/red light illumination and subjected to immunoblotting. e Immunofluorescence staining of the indicated photoreceptors in MSCs