VIP Receptor 1 Antibody - #DF5172

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製品: VIP Receptor 1 Antibody
カタログ: DF5172
タンパク質の説明: Rabbit polyclonal antibody to VIP Receptor 1
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Chicken
分子量: 52 KD; 52kD(Calculated).
ユニプロット: P32241
RRID: AB_2837521

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(89%), Bovine(89%), Horse(89%), Rabbit(89%), Chicken(89%)
クローナリティ:
Polyclonal
特異性:
VIP Receptor 1 Antibody detects endogenous levels of total VIP Receptor 1.
RRID:
AB_2837521
引用形式: Affinity Biosciences Cat# DF5172, RRID:AB_2837521.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ41949; HVR 1; HVR1; PACAP R 2; PACAP type II receptor; PACAP-R-2; PACAP-R2; Pituitary adenylate cyclase activating polypeptide receptor type II; Pituitary adenylate cyclase activating polypeptide type II receptor; Pituitary adenylate cyclase-activating polypeptide type II receptor; RCD1; RDC 1; RDC1; V1RG; VAPC 1; VAPC1; Vasoactive intestinal peptide receptor 1; Vasoactive intestinal polypeptide receptor 1; Vasoactive intestinal polypeptide receptor 1 precursor; VIP and PACAP receptor 1; VIP R 1; VIP receptor type I; VIP-R-1; VIPR 1; VIPR; VIPR1; VIPR1_HUMAN; VIRG; VPAC 1; VPAC1; VPCAP1R;

免疫原

免疫原:
Uniprot:

P32241(VIPR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
VIPR1(7433)
発現特異性:
P32241 VIPR1_HUMAN:

In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T-cell lines Peer, MOLT-4, HSB and YT.

タンパク質配列:
MRPPSPLPARWLCVLAGALAWALGPAGGQAARLQEECDYVQMIEVQHKQCLEEAQLENETIGCSKMWDNLTCWPATPRGQVVVLACPLIFKLFSSIQGRNVSRSCTDEGWTHLEPGPYPIACGLDDKAASLDEQQTMFYGSVKTGYTIGYGLSLATLLVATAILSLFRKLHCTRNYIHMHLFISFILRAAAVFIKDLALFDSGESDQCSEGSVGCKAAMVFFQYCVMANFFWLLVEGLYLYTLLAVSFFSERKYFWGYILIGWGVPSTFTMVWTIARIHFEDYGCWDTINSSLWWIIKGPILTSILVNFILFICIIRILLQKLRPPDIRKSDSSPYSRLARSTLLLIPLFGVHYIMFAFFPDNFKPEVKMVFELVVGSFQGFVVAILYCFLNGEVQAELRRKWRRWHLQGVLGWNPKYRHPSGGSNGATCSTQVSMLTRVSPGARRSSSFQAEVSLV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
89
Bovine
89
Chicken
89
Rabbit
89
Dog
78
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P32241 基板として

Site PTM Type Enzyme
S165 Phosphorylation
S250 Phosphorylation
T429 Phosphorylation
S435 Phosphorylation
S447 Phosphorylation
S448 Phosphorylation
S449 Phosphorylation
S455 Phosphorylation

研究背景

機能:

This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T-cell lines Peer, MOLT-4, HSB and YT.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 2 family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

参考文献

1). Modular Screening Reveals Driver Induced Additive Mechanisms of Baicalin and Jasminoidin on Cerebral Ischemia Therapy. Frontiers in Cardiovascular Medicine, 2022 (PubMed: 35265682) [IF=3.6]

Application: WB    Species: Rat    Sample: hippocampus tissue

Figure 7 Validation of the driver proteins' expression level in each group. (A) The band diagrams of 7 representative driver proteins and β-actin in each group. (B–H) The expression level comparison among groups for PAQR8, RhoA, EMC10, GGA2, VIPR1, and FAM120A, SEMA3F determined by western blot. *p < 0.05, **p < 0.01, ***p < 0.001, and ns, non-significant.

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