CD63 Antibody - #AF5117

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製品: CD63 Antibody
カタログ: AF5117
タンパク質の説明: Rabbit polyclonal antibody to CD63
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
分子量: 25kDa,47kDa; 26kD(Calculated).
ユニプロット: P08962
RRID: AB_2837603

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HPLC Report
MSDS
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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
CD63 Antibody detects endogenous levels of total CD63.
RRID:
AB_2837603
引用形式: Affinity Biosciences Cat# AF5117, RRID:AB_2837603.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Lysosomal associated membrane protein 3; CD 63; CD63; CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 antigen melanoma 1 antigen; CD63 molecule; CD63_HUMAN; gp55; Granulophysin; LAMP 3; LAMP-3; LAMP3; LIMP; Lysosomal-associated membrane protein 3; Lysosome associated membrane glycoprotein 3; Mast cell antigen AD1; ME491; Melanoma 1 antigen; Melanoma associated antigen ME491; Melanoma associated antigen MLA1; Melanoma-associated antigen ME491; MGC72893; MLA 1; MLA1; NGA; Ocular melanoma associated antigen; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin 30; Tetraspanin-30; Tspan 30; Tspan-30; TSPAN30;

免疫原

免疫原:
Uniprot:

P08962(CD63_HUMAN) >>Visit HPA database.

遺伝子(ID):
CD63(967)
発現特異性:
P08962 CD63_HUMAN:

Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

タンパク質の説明:
Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGF
タンパク質配列:
MAVEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTIIQGATPGSLLPVVIIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACCLVKSIRSGYEVM

PTMs - P08962 基板として

Site PTM Type Enzyme
K110 Ubiquitination
K128 Ubiquitination
N130 N-Glycosylation
S159 Phosphorylation
K162 Ubiquitination
K188 Ubiquitination
K194 Ubiquitination

研究背景

機能:

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

PTMs:

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Lysosome membrane>Multi-pass membrane protein. Late endosome membrane>Multi-pass membrane protein. Endosome>Multivesicular body. Melanosome. Secreted>Extracellular exosome. Cell surface.
Note: Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

サブユニット構造:

Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1. Interacts with CD9. Identified in a complex with CD9 and ITGB3. Interacts with PMEL. Interacts with KDR/VEGFR2; identified in a complex with ITGB1 and KDR/VEGFR2 and is required to recruit KDR to ITGB1 complexes. Interacts with SYT7 (By similarity).

タンパク質ファミリー:

Belongs to the tetraspanin (TM4SF) family.

研究領域

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

参考文献

1). Exosomes-loaded electroconductive nerve dressing for nerve regeneration and pain relief against diabetic peripheral nerve injury. Bioactive Materials, 2023 (PubMed: 36923267) [IF=18.9]
2). Iron Oxide Nanoparticles Engineered Macrophage-Derived Exosomes for Targeted Pathological Angiogenesis Therapy. ACS nano, 2024 (PubMed: 38412252) [IF=15.8]

Application: WB    Species: Mouse    Sample:

Figure 2 Characterization of ESIONPs@EXO derived from ESIONPs engineered macrophages. (A) The morphology of EXO and ESIONPs@EXO determined by TEM. Scale bar: 200 nm (left) and 100 nm (right). (B) The size distribution of EXO and ESIONPs@EXO evaluated by NTA. (C) Western blot analysis of CD9, CD63, CD81, TSG101, and calnexin. (D) Relaxation properties of ESIONPs@EXO. (E) T1 and T2 weighted MR images of ESIONPs@EXO at different concentrations (measured on a 3 T MR scanner). 1/T1 (F) and 1/T2 (G) relaxation rates of ESIONPs@EXO at different concentrations.
3). Exosomal miR-21 from tubular cells contributes to renal fibrosis by activating fibroblasts via targeting PTEN in obstructed kidneys. Theranostics, 2023 (PubMed: 34522205) [IF=12.4]

Application: WB    Species: Mice    Sample: NRK-52E cells

Figure 2 TGF-β1 promotes the secretion of exosomes by renal tubular epithelial cells and activates fibroblasts in vitro. (A) Schematic diagram of experimental process. Exosomes from NRK-52E cells treated without (Ctrl-Exos) or with TGF-β1 (TGFβ1-Exos) were extracted and incubated with NRK-49F cells. (B,C) DLS and NTA of exosomes from NRK-52E cells. (D) TEM image of exosomes isolated from NRK-52E cells. Scale bar = 100 nm. (E, F) Representative western blot (E) and quantitative data (F) of CD63 as an exosome marker in exosomes from TGF-β1- or GW4869-treated NRK-52E cells. Numbers (1 to 3) indicate each independent treatment in the given group. *p < 0.05 versus Ctrl-Exos, #p < 0.05 versus 5 ng/ml TGFβ1-Exos, &p < 0.05 versus 15 ng/ml TGFβ1-Exos (n = 3). (G) Fluorescent staining image of PKH-67-labeled NRK-52E cells. Scales bars=50 μm. (H) Fluorescent staining image of NRK-52E cell-derived exosomes taken up by NRK-49F cells. Scales bars=10 μm. (I, K) Representative western blot (I) and quantitative data (K) of α-SMA and PCNA in NRK-49F cells incubated with exosomes from TGF-β1- or GW4869-treated NRK-52E cells. Numbers (1 to 3) indicate each independent treatment in the giving group. *p < 0.05 versus Ctrl-Exos, #p < 0.05 versus 5 ng/ml TGFβ1-Exos, &p < 0.05 versus 15 ng/ml TGFβ1-Exos (n = 3). (J) Proliferation rate of NRK-49F cells incubated with NRK-52E cell-derived exosomes measured by CCK-8. *p < 0.05 versus Ctrl-Exos, #p < 0.05 versus 5 ng/ml TGFβ1-Exos, &p < 0.05 versus 15 ng/mL TGFβ1-Exos (n = 3). (L-N) Double immunofluorescence staining (green for Col-I and red for fibronectin) demonstrates the expression of Col-I and fibronectin in NRK-49F cells incubated with NRK-52E cell-derived exosomes. Scales bars=50 μm. *p < 0.05 versus Ctrl-Exos, #p < 0.05 versus 5 ng/ml TGFβ1-Exos, &p < 0.05 versus 15 ng/mL TGFβ1-Exos.
4). Small extracellular vesicles encapsulating lefty1 mRNA inhibit hepatic fibrosis. Asian Journal of Pharmaceutical Sciences, 2022 (PubMed: 36382306) [IF=10.7]
5). Regulatory T cell-derived exosome mediated macrophages polarization for osteogenic differentiation in fracture repair. Journal of controlled release : official journal of the Controlled Release Society, 2024 (PubMed: 38508525) [IF=10.5]
6). Tubular cell-derived exosomal miR-150-5p contributes to renal fibrosis following unilateral ischemia-reperfusion injury by activating fibroblast in vitro and in vivo. International Journal of Biological Sciences, 2023 (PubMed: 34671216) [IF=8.2]

Application: IF/ICC    Species: Mice    Sample: kidney

Figure 2 Increased secretion of exosomes in the kidney following UIRI. (A) Immunofluorescence staining for CD63 and TSG-101 (red). (B) Integrated optical density (IOD) of TSG-101. (C) Integrated optical density (IOD) of CD63. (D) Double immunofluorescence staining for CD63 (red) and AQP-1 (green), respectively. (E) Western blotting of CD63 in kidney tissue and exosome at different time points after UIRI (n=6). (F) Quantitative of CD63 in kidney. (G) Quantitative of CD63 in exosome. (H) TEM image of the exosomes in the kidney tissue after UIRI. *P < 0.05 versus sham.

Application: WB    Species: Mice    Sample: kidney

Figure 2 Increased secretion of exosomes in the kidney following UIRI. (A) Immunofluorescence staining for CD63 and TSG-101 (red). (B) Integrated optical density (IOD) of TSG-101. (C) Integrated optical density (IOD) of CD63. (D) Double immunofluorescence staining for CD63 (red) and AQP-1 (green), respectively. (E) Western blotting of CD63 in kidney tissue and exosome at different time points after UIRI (n=6). (F) Quantitative of CD63 in kidney. (G) Quantitative of CD63 in exosome. (H) TEM image of the exosomes in the kidney tissue after UIRI. *P < 0.05 versus sham.
7). MicroRNA-enriched small extracellular vesicles possess odonto-immunomodulatory properties for modulating the immune response of macrophages and promoting odontogenesis. Stem Cell Research & Therapy, 2020 (PubMed: 33256846) [IF=7.5]

Application: WB    Species: Human    Sample: DPSCs-sEV

Fig. 1 Identification and characterization of DPSCs-sEV. a The morphology of DPSCs-sEV was determined by TEM, scale bar = 50 nm. b Expression of CD9 and CD63 in the DPSCs-sEV (sEV represents DPSCs-sEV and Lys represents DPSCs lysate, 尾-actin is a control for the lysate). c Nano-flow cytometry showed DPSCs-sEV ranged between 30 and 150 nm in diameter. d PKH26-labeled DPSCs-sEV were found in macrophage cytosol
8). Chemerin-Induced Down-Regulation of Placenta-Derived Exosomal miR-140-3p and miR-574-3p Promotes Umbilical Vein Endothelial Cells Proliferation, Migration, and Tube Formation in Gestational Diabetes Mellitus. Cells, 2022 (PubMed: 36359855) [IF=6.0]
9). Adipocyte-derived exosomes from obstructive sleep apnoea rats aggravate MASLD by TCONS_00039830/miR-455-3p/Smad2 axis. Communications biology, 2024 (PubMed: 38654054) [IF=5.9]
10). Exosomes derived from dental pulp stem cells accelerate cutaneous wound healing by enhancing angiogenesis via the Cdc42/p38 MAPK pathway. International Journal of Molecular Medicine, 2022 (PubMed: 36321793) [IF=5.7]
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