Caspase 7 Antibody - #AF5118
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5118, RRID:AB_2837604.
折りたたみ／展開
Apoptotic protease Mch-3; Apoptotic protease MCH3; CASP-7; CASP7; CASP7_HUMAN; Caspase 7; Caspase 7 apoptosis related cysteine peptidase; Caspase-7 subunit p11; Caspase7; CMH 1; CMH-1; CMH1; ICE LAP3; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; MCH3;
免疫原
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
- P55210 CASP7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADDQGCIEEQGVEDSANEDSVDAKPDRSSFVPSLFSKKKKNVTMRSIKTTRDRVPTYQYNMNFEKLGKCIIINNKNFDKVTGMGVRNGTDKDAEALFKCFRSLGFDVIVYNDCSCAKMQDLLKKASEEDHTNAACFACILLSHGEENVIYGKDGVTPIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQADSGPINDTDANPRYKIPVEADFLFAYSTVPGYYSWRSPGRGSWFVQALCSILEEHGKDLEIMQILTRVNDRVARHFESQSDDPHFHEKKQIPCVVSMLTKELYFSQ
PTMs - P55210 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| S34 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| K38 | Methylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| T44 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| T157 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| T173 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| K177 | Ubiquitination | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Q13177 (PAK2) | Uniprot |
研究背景
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
Cytoplasm.
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Interacts with ATXN3 (short isoform 1).
Belongs to the peptidase C14A family.
研究領域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Bacterial > Legionellosis.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
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