Caspase 7 Antibody - #AF5118

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製品: Caspase 7 Antibody
カタログ: AF5118
タンパク質の説明: Rabbit polyclonal antibody to Caspase 7
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Rat
分子量: 34 kDa; 34kD(Calculated).
ユニプロット: P55210
RRID: AB_2837604

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
クローナリティ:
Polyclonal
特異性:
Caspase 7 Antibody detects endogenous levels of total Caspase 7.
RRID:
AB_2837604
引用形式: Affinity Biosciences Cat# AF5118, RRID:AB_2837604.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Apoptotic protease Mch-3; Apoptotic protease MCH3; CASP-7; CASP7; CASP7_HUMAN; Caspase 7; Caspase 7 apoptosis related cysteine peptidase; Caspase-7 subunit p11; Caspase7; CMH 1; CMH-1; CMH1; ICE LAP3; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; MCH3;

免疫原

免疫原:

A synthesized peptide derived from human Caspase 7, corresponding to a region within N-terminal amino acids.

Uniprot:

P55210(CASP7_HUMAN) >>Visit HPA database.

遺伝子(ID):
CASP7(840)
発現特異性:
P55210 CASP7_HUMAN:

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

タンパク質の説明:
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.
タンパク質配列:
MADDQGCIEEQGVEDSANEDSVDAKPDRSSFVPSLFSKKKKNVTMRSIKTTRDRVPTYQYNMNFEKLGKCIIINNKNFDKVTGMGVRNGTDKDAEALFKCFRSLGFDVIVYNDCSCAKMQDLLKKASEEDHTNAACFACILLSHGEENVIYGKDGVTPIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQADSGPINDTDANPRYKIPVEADFLFAYSTVPGYYSWRSPGRGSWFVQALCSILEEHGKDLEIMQILTRVNDRVARHFESQSDDPHFHEKKQIPCVVSMLTKELYFSQ

研究背景

機能:

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.

PTMs:

Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

タンパク質ファミリー:

Belongs to the peptidase C14A family.

研究領域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

参考文献

1). RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs. European journal of medical research, 2025 (PubMed: 41013641) [IF=2.8]

Application: WB    Species: human    Sample:

Fig. 5 RHBDD1 Regulates DNA-PKcs and p53 Levels in an ERAD-Dependent Manner. A ERAD marker analysis in sh-RHBDD1- or pcDNA-RHBDD1-transfected cells treated with STL127705 or Nutlin-3a, assessed by western blotting. B Relative protein levels in sh-RHBDD1 transfected cells. C Apoptosis induced by cisplatin in pcDNA-RHBDD1-transfected A549 and NCI-H596 cells. Data are shown as mean ± SD. N = 3 for western blot analysis. *p 
2). Pazopanib attenuated bleomycin-induced pulmonary fibrosis via suppressing TGF-β1 signaling pathway. Journal of thoracic disease, 2024 (PubMed: 38738240) [IF=2.1]

Application: WB    Species: Mouse    Sample: Mlg cells

Figure 4. PZ promotes apoptosis and autophagy of pulmonary myofibroblast. (A) The Mlg cells were incubated with TGF-β1 and/or PZ (2 or 4 μM) for 24 hours. The expression levels of caspase3, cleaved-caspase3, caspase7, cleaved-caspase7, caspase9 and cleaved-caspase9, were detected via using Western blot. The loading control was β-tubulin. (B,C) Mlg cells were exposed to Baf a1/CQ and/or PZ (2 or 4 μM) for 24 hours. The p62 expression level was evaluated by Western blot. GAPDH was used as a loading control. (D,E) The plasmid of GFP-LC3B and GFP-Cherry-LC3B were transfected to NIH-3T3 with PEI, and fluorescence microscopy was used to examine the number of autophagosomes (green puncta) and autolysosome (red puncta) after exposing to TGF-β1 and/or PZ for 12 hours. Scale bars: 50 µm. Data in (A-C) are mean ± standard deviation. ###, P

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