SIRT3 Antibody - #AF5135

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製品: SIRT3 Antibody
カタログ: AF5135
タンパク質の説明: Rabbit polyclonal antibody to SIRT3
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC, IF/ICC
反応性: Human, Mouse, Rat
予測: Sheep, Rabbit
分子量: 29 kDa; 44kD(Calculated).
ユニプロット: Q9NTG7
RRID: AB_2837621

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Sheep(88%), Rabbit(88%)
クローナリティ:
Polyclonal
特異性:
SIRT3 Antibody detects endogenous levels of total SIRT3.
RRID:
AB_2837621
引用形式: Affinity Biosciences Cat# AF5135, RRID:AB_2837621.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

hSIRT 3; hSIRT3; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase; NAD dependent deacetylase sirtuin 3 mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; Regulatory protein SIR2 homolog 3; Silent mating type information regulation 2 S.cerevisiae homolog 3; Sir 2 like 3; SIR 2 like protein 3; SIR 3; SIR2 L3; Sir2 like 3; SIR2 like protein 3; SIR2-like protein 3; SIR2L3; SIR3_HUMAN; SIRT 3; SIRT3; Sirtuin 3; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae); Sirtuin type 3; Sirtuin3;

免疫原

免疫原:

A synthesized peptide derived from human SIRT3, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9NTG7(SIR3_HUMAN) >>Visit HPA database.

遺伝子(ID):
SIRT3(23410)
発現特異性:
Q9NTG7 SIR3_HUMAN:

Widely expressed.

タンパク質の説明:
NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
タンパク質配列:
MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRDDVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
88
Sheep
88
Pig
75
Dog
75
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity).

PTMs:

Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.

細胞の位置付け:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed.

タンパク質ファミリー:

Belongs to the sirtuin family. Class I subfamily.

研究領域

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

参考文献

1). β-hydroxybutyrylation and O-GlcNAc modifications of STAT1 modulate antiviral defense in aging. Cellular & molecular immunology, 2025 (PubMed: 39979583) [IF=21.8]
2). Sirt3-mediated mitophagy regulates AGEs-induced BMSCs senescence and senile osteoporosis. Redox Biology, 2021 (PubMed: 33662874) [IF=10.7]

Application: WB    Species: mice    Sample: bone marrow mesenchymal stem (BMSCs)

Fig. 2. Effects of different concentrations of AGEs on mitochondrial function and mitophagy of BMSCs. The BMSCs were treated with AGEs (50–200 μg/mL) or BSA for 24–72 h. (A) Representative fluorescence images with DCF (green) staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (B) Representative fluorescence images with Mito-SOX (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (C) The MMP was detected through JC-1 staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (D) Representative fluorescence images with Mtphagy Dye (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (E) Representative fluorescence images with LC3B (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (F) Representative Western blotting assay and quantitation of the level of LC3B, P62, Parkin, Sirt3. **p < 0.01 versus BSA. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
3). The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3. Redox biology, 2023 (PubMed: 37099926) [IF=10.7]
4). Vine tea (Ampelopsis grossedentata) ameliorates chronic alcohol-induced hepatic steatosis, oxidative stress, and inflammation via YTHDF2/PGC-1α/SIRT3 axis. FOOD RESEARCH INTERNATIONAL, 2025 [IF=8.0]
5). Vine tea (Ampelopsis grossedentata) ameliorates chronic alcohol-induced hepatic steatosis, oxidative stress, and inflammation via YTHDF2/PGC-1α/SIRT3 axis. Food research international (Ottawa, Ont.), 2025 (PubMed: 40253212) [IF=7.0]
6). Inhibiting mir-34a-5p regulates doxorubicin-induced autophagy disorder and alleviates myocardial pyroptosis by targeting Sirt3-AMPK pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023 (PubMed: 37806095) [IF=6.9]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 6. Sirt3 is the direct target of mir-34a-5p. (A) Schematic diagram of binding sites between Mir-34a-5p and Sirt3. (B) The luciferase reporter assays of Mir-34a-5p and Sirt3 (n = 3). (C) Fluorescence of Sirt3 using the inhibitors or mimics of Mir-34a-5p. (D) Quantification the fluorescene of Sirt3 (n = 5). The values are expressed as mean ± SD. NS, not significant, *P 

Application: WB    Species: Rat    Sample: H9C2 cell

Fig. 7. Overexpression of sirt3 alleviates DOX-induced H9C2 cell autophagy and pyroptosis in vitro. (A) Protein level of Sirt3 using SiRNA or plasmid as measured by western blotting and (B) Quantification the expression of Sirt3 (n = 3). (C) Protein level of Sirt3 using siRNA or plasmid as measured by RT-PCR (n = 3). (D) Use SiRNA or Sirt3 plasmid to observe H9C2 cell viability by CCK-8 assay (n = 5). (E) Representative western blots using SiRNA and (F) Quantification the expression of proteins (n = 3). (G) Representative western blots using Sirt3 plasmid and (H) Quantification the expression of proteins (n = 3). (I, J) Detection and quantification of MitoROS (n = 5). (K, L) Detection and quantification of autophagic flux (n = 5). The values are expressed as mean ± SD. NS, not significant, *P 
7). Baicalin Attenuates Diabetic Cardiomyopathy In Vivo and In Vitro by Inhibiting Autophagy and Cell Death Through SENP1/SIRT3 Signaling Pathway Activation. Antioxidants & redox signaling, 2025 (PubMed: 38687336) [IF=5.9]
8). 3-MCPD Induced Mitochondrial Damage of Renal Cells Via the Rhythmic Protein BMAL1 Targeting SIRT3/SOD2. Journal of Agricultural and Food Chemistry, 2023 (PubMed: 37750480) [IF=5.7]
9). PGC-1α activation ameliorates cancer-induced bone pain via inhibiting apoptosis of GABAergic interneurons. Biochemical pharmacology, 2024 (PubMed: 38354958) [IF=5.3]
10). SIRT3-and FAK-mediated acetylation-phosphorylation crosstalk of NFATc1 regulates Nε-carboxymethyl-lysine-induced vascular calcification in diabetes mellitus. Atherosclerosis, 2023 (PubMed: 37392543) [IF=4.9]
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