SIRT3 Antibody - #AF5135

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製品: SIRT3 Antibody
カタログ: AF5135
タンパク質の説明: Rabbit polyclonal antibody to SIRT3
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Sheep, Rabbit
分子量: 29 kDa; 44kD(Calculated).
ユニプロット: Q9NTG7
RRID: AB_2837621

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WB Handbook
IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Sheep(88%), Rabbit(88%)
クローナリティ:
Polyclonal
特異性:
SIRT3 Antibody detects endogenous levels of total SIRT3.
RRID:
AB_2837621
引用形式: Affinity Biosciences Cat# AF5135, RRID:AB_2837621.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

hSIRT 3; hSIRT3; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase; NAD dependent deacetylase sirtuin 3 mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; Regulatory protein SIR2 homolog 3; Silent mating type information regulation 2 S.cerevisiae homolog 3; Sir 2 like 3; SIR 2 like protein 3; SIR 3; SIR2 L3; Sir2 like 3; SIR2 like protein 3; SIR2-like protein 3; SIR2L3; SIR3_HUMAN; SIRT 3; SIRT3; Sirtuin 3; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae); Sirtuin type 3; Sirtuin3;

免疫原

免疫原:
Uniprot:

Q9NTG7(SIR3_HUMAN) >>Visit HPA database.

遺伝子(ID):
SIRT3(23410)
発現特異性:
Q9NTG7 SIR3_HUMAN:

Widely expressed.

タンパク質の説明:
NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
タンパク質配列:
MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRDDVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
88
Sheep
88
Pig
75
Dog
75
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NTG7 基板として

Site PTM Type Enzyme
R51 Methylation
R57 Methylation
S105 Phosphorylation
S110 Phosphorylation
T150 Phosphorylation P06493 (CDK1)
S159 Phosphorylation P06493 (CDK1)
R276 Methylation
T284 Phosphorylation
R356 Methylation
T391 Phosphorylation

研究背景

機能:

NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity).

PTMs:

Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.

細胞の位置付け:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed.

サブユニット構造:

Upon metabolic stress, forms a complex composed of FOXO3, SIRT3 and mitochondrial RNA polymerase POLRMT; the complex is recruited to mtDNA in a SIRT3-dependent manner. Also forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT. Interacts with NDUFA9, ACSS1, IDH2 and GDH. Interacts with PCCA.

タンパク質ファミリー:

Belongs to the sirtuin family. Class I subfamily.

研究領域

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

参考文献

1). Sirt3-mediated mitophagy regulates AGEs-induced BMSCs senescence and senile osteoporosis. Redox Biology, 2021 (PubMed: 33662874) [IF=11.4]

Application: WB    Species: mice    Sample: bone marrow mesenchymal stem (BMSCs)

Fig. 2. Effects of different concentrations of AGEs on mitochondrial function and mitophagy of BMSCs. The BMSCs were treated with AGEs (50–200 μg/mL) or BSA for 24–72 h. (A) Representative fluorescence images with DCF (green) staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (B) Representative fluorescence images with Mito-SOX (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (C) The MMP was detected through JC-1 staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (D) Representative fluorescence images with Mtphagy Dye (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (E) Representative fluorescence images with LC3B (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (F) Representative Western blotting assay and quantitation of the level of LC3B, P62, Parkin, Sirt3. **p < 0.01 versus BSA. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
2). 3-MCPD Induced Mitochondrial Damage of Renal Cells Via the Rhythmic Protein BMAL1 Targeting SIRT3/SOD2. Journal of Agricultural and Food Chemistry, 2023 (PubMed: 37750480) [IF=6.1]
3). PGC-1α activation ameliorates cancer-induced bone pain via inhibiting apoptosis of GABAergic interneurons. Biochemical pharmacology, 2024 (PubMed: 38354958) [IF=5.8]
4). Oxyberberine suppressed the carbon tetrachloride-induced liver fibrosis by inhibiting liver inflammation in a sirtuin 3-dependent manner in mice. International immunopharmacology, 2023 (PubMed: 37599565) [IF=5.6]
5). Promising anti-ovarian aging herbal formulation He's Yangchao promotes in vitro maturation of oocytes from advanced maternal age mice. Journal of Ethnopharmacology, 2024 (PubMed: 37423514) [IF=5.4]
6). SIRT3-and FAK-mediated acetylation-phosphorylation crosstalk of NFATc1 regulates Nε-carboxymethyl-lysine-induced vascular calcification in diabetes mellitus. Atherosclerosis, 2023 (PubMed: 37392543) [IF=5.3]
7). Single-walled carbon nanohorn aggregates promotes mitochondrial dysfunction-induced apoptosis in hepatoblastoma cells by targeting SIRT3. INTERNATIONAL JOURNAL OF ONCOLOGY, 2018 (PubMed: 29956732) [IF=5.2]

Application: WB    Species: mouse    Sample: HepG2 cell

Figure 3.| Treatment with SWNHs alters the expression of mitochondrial apoptotic pathway-associated proteins in vitro. (A) HepG2 and (B) L02 cells were incubated with different concentrations of SWNHs in 6-well plates for 48 h. The changes in the expression and the relative quantification of proteins in (C) HepG2 cells or (D) L02 cells were identified by western blotting. SW0, SW10, SW20, SW30, SW40 and SW50 correspond to the concentrations of SWNHs: 0, 0.21, 0.42, 0.64, 0.85 and 0.96 µg/cm2, respectively. Data are presented as the mean ± standard deviation (n=3). *P<0.05 compared with the SW0 group. AceCS2, acyl-CoA synthetase short chain family member 1; SCNN1A, sodium channel epithelial 1α subunit; SIRT3, sirtuin 3; SWNH, single-walled carbon nanohorn; VDAC1, voltage-dependent anion channel 1.
8). Activation of SIRT3 by Tanshinone IIA ameliorates renal fibrosis by suppressing the TGF-β/TSP-1 pathway and attenuating oxidative stress. Cellular signalling, 2024 (PubMed: 39153586) [IF=4.8]
9). miR-494 induces EndMT and promotes the development of HCC (Hepatocellular Carcinoma) by targeting SIRT3/TGF-β/SMAD signaling pathway. Scientific Reports, 2019 (PubMed: 31076630) [IF=4.6]

Application: IHC    Species: human    Sample: non-tumorous, para-tumorous, and tumorous tissues

Figure 5.|SIRT3 expression in non-tumorous, para-tumorous, and tumorous tissues. Te immunohistochemistry results of liver tissues showed that SIRT3 was positive in all cases. However, the positive intensity and express location in diferent groups were diferences. As showed in this Figure, SIRT3 mainly appeared in the cytoplasm in tumor tissue, but they were at the same time appearing in the cytoplasm and nucleus of para-tumorous and non-tumorous tissues. Moreover, the positive intensity of tumor tissue much lower than para-tumorous or non-tumorous tissues. Te diferences of SIRT3 positive intensity between tumorous and para-tumorous or non-tumorous tissue were statistically signifcant. Te data are presented as means ± SD from three independent experiments. ***P < 0.001.
10). O-GlcNAcylation orchestrates porcine oocyte maturation through maintaining mitochondrial dynamics and function. Molecular human reproduction, 2024 (PubMed: 38265252) [IF=4.0]
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