Abi 1 Antibody - #AF5158
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5158, RRID:AB_2837644.
折りたたみ／展開
Abelson interactor 1; Abi-1; Abi1; ABI1_HUMAN; Abl binding protein 4; Abl interactor 1; Abl interactor protein 1; Abl-binding protein 4; AblBP4; e3B1; Eps8 binding protein; Eps8 SH3 domain binding protein; Eps8 SH3 domain-binding protein; Eps8-binding protein; Hssh3bp1; Interactor protein AblBP4; NAP1; Nap1 binding protein; Nap1-binding protein; Nap1BP; Spectrin SH3 domain binding protein 1; Spectrin SH3 domain-binding protein 1; SSH3BP;
免疫原
- Q8IZP0 ABI1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAELQMLLEEEIPSGKRALIESYQNLTRVADYCENNYIQATDKRKALEETKAYTTQSLASVAYQINALANNVLQLLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANMERPVRYIRKPIDYTVLDDVGHGVKWLKAKHGNNQPARTGTLSRTNPPTQKPPSPPMSGRGTLGRNTPYKTLEPVKPPTVPNDYMTSPARLGSQHSPGRTASLNQRPRTHSGSSGGSGSRENSGSSSIGIPIAVPTPSPPTIGPENISVPPPSGAPPAPPLAPLLPVSTVIAAPGSAPGSQYGTMTRQISRHNSTTSSTSSGGYRRTPSVTAQFSAQPHVNGGPLYSQNSISIAPPPPPMPQLTPQIPLTGFVARVQENIADSPTPPPPPPPDDIPMFDDSPPPPPPPPVDYEDEEAAVVQYNDPYADGDPAWAPKNYIEKVVAIYDYTKDKDDELSFMEGAIIYVIKKNDDGWYEGVCNRVTGLFPGNYVESIMHYTD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IZP0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| Y23 | Phosphorylation | Uniprot | |
| Y37 | Phosphorylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| K123 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| Y143 | Phosphorylation | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| T174 | Phosphorylation | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S187 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| T196 | Phosphorylation | Uniprot | |
| Y198 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| Y213 | Phosphorylation | P00519 (ABL1) | Uniprot |
| T215 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | P28482 (MAPK1) , P06493 (CDK1) , P27361 (MAPK3) | Uniprot |
| S222 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S225 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| T229 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| T238 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S267 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S319 | Phosphorylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| T328 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| Y333 | Phosphorylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| Y355 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| T394 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S410 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| Y421 | Phosphorylation | Uniprot | |
| Y431 | Phosphorylation | Uniprot | |
| Y435 | Phosphorylation | Uniprot | |
| Y455 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| Y484 | Phosphorylation | Uniprot | |
| Y499 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| Y506 | Phosphorylation | Uniprot | |
| T507 | Phosphorylation | Uniprot |
研究背景
May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.
Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. Seems to be phosphorylated at Tyr-53 by ABL1, required for nuclear but not for synaptic localization.
Cytoplasm. Nucleus. Cell projection>Lamellipodium. Cell projection>Filopodium. Cell projection>Growth cone. Cell junction>Synapse>Postsynaptic density. Cytoplasm>Cytoskeleton.
Note: Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. May shuttle from the postsynaptic densities to the nucleus (By similarity).
Widely expressed, with highest expression in brain.
Interacts with ABL1, ENAH, STX1A, SNAP25, VAMP2, EPS8, and through its N-terminus with WASF1. Part of a complex consisting of ABI1, STX1A and SNAP25. Part of a complex consisting of ABI1, EPS8 and SOS1 (By similarity). Interacts with SOS1, SOS2, GRB2, SPTA1 and the first SH3 domain of NCK1. Isoform 6 does not interact with NCK1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 (NCKAP1l/HEM1 in hematopoietic cells) and WASF2/WAVE2. Interacts (via SH3 domain) with SHANK2 and SHANK3, but not SHANK1; the interaction is direct. Interacts with the heterodimer MYC:MAX; the interaction may enhance MYC:MAX transcriptional activity. Interacts with FNBP1L (via the SH3 domain), WASF2, and CDC42, but only in the presence of FNBP1L.
(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL135.
The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A.
Belongs to the ABI family.
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