BMP4 Antibody - #AF5175

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製品: BMP4 Antibody
カタログ: AF5175
タンパク質の説明: Rabbit polyclonal antibody to BMP4
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Bovine, Sheep, Rabbit, Dog
分子量: 46 kDa; 47kD(Calculated).
ユニプロット: P12644
RRID: AB_2837661

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(93%), Sheep(93%), Rabbit(86%), Dog(93%)
クローナリティ:
Polyclonal
特異性:
BMP4 Antibody detects endogenous levels of total BMP4.
RRID:
AB_2837661
引用形式: Affinity Biosciences Cat# AF5175, RRID:AB_2837661.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

zgc:100779; BMP 2B; BMP 4; BMP-2B; BMP-4; BMP2B; BMP2B1; BMP4; BMP4_HUMAN; Bone morphogenetic protein 2B; Bone morphogenetic protein 4; DVR4; MCOPS6; MGC100779; OFC11; zbmp-4; ZYME;

免疫原

免疫原:
Uniprot:

P12644(BMP4_HUMAN) >>Visit HPA database.

遺伝子(ID):
BMP4(652)
発現特異性:
P12644 BMP4_HUMAN:

Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.

タンパク質の説明:
Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction
タンパク質配列:
MIPGNRMLMVVLLCQVLLGGASHASLIPETGKKKVAEIQGHAGGRRSGQSHELLRDFEATLLQMFGLRRRPQPSKSAVIPDYMRDLYRLQSGEEEEEQIHSTGLEYPERPASRANTVRSFHHEEHLENIPGTSENSAFRFLFNLSSIPENEVISSAELRLFREQVDQGPDWERGFHRINIYEVMKPPAEVVPGHLITRLLDTRLVHHNVTRWETFDVSPAVLRWTREKQPNYGLAIEVTHLHQTRTHQGQHVRISRSLPQGSGNWAQLRPLLVTFGHDGRGHALTRRRRAKRSPKHHSQRARKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
93
Sheep
93
Dog
93
Rabbit
86
Chicken
71
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12644 基板として

Site PTM Type Enzyme
Y82 Phosphorylation
S91 Phosphorylation
T116 Phosphorylation
S119 Phosphorylation
K185 Ubiquitination
K228 Ubiquitination

研究背景

機能:

Induces cartilage and bone formation. Also acts in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).

細胞の位置付け:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.

サブユニット構造:

Homodimer; disulfide-linked (By similarity). Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with SOSTDC1. Interacts with FBN1 (via N-terminal domain) and FBN2.

タンパク質ファミリー:

Belongs to the TGF-beta family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

参考文献

1). Effect of LncRNA-MALAT1 on mineralization of dental pulp cells in a high-glucose microenvironment. Frontiers in Cell and Developmental Biology, 2022 (PubMed: 36035997) [IF=5.5]

Application: WB    Species: Human    Sample:

FIGURE 2 Effects of a high-glucose microenvironment on mineralization of DPCs. (A,C) NC group 7 /14 days after induced mineralization stained by alizarin red; (B,D) high-glucose group 7 /14 days after induced mineralization stained by alizarin red; (E) semi-quantitative analysis of alizarin red staining; (F) expression of mineralization-related factors was detected by qRT-PCR after 7 days of DPCs which were cultured in a high-glucose microenvironment; (G) expression of mineralization-related factors was detected by qRT-PCR after 14 days; (H) expression results of mineralization-related factors detected by Western blot after 7 /14 days of DPC induction in a high-glucose microenvironment; (I,J) density ratio of target proteins to GAPDH on 7 days and 14 days (n = 3, scale bar = 500 μm # p < 0.05, *p < 0.01).
2). TMF suppresses chondrocyte hypertrophy in osteoarthritic cartilage by mediating the FOXO3a/BMPER pathway. Experimental and therapeutic medicine, 2024 (PubMed: 38800044) [IF=2.7]

Application: WB    Species: Human    Sample: C28/I2 cells

Figure 2 Effects of TMF on chondrocyte hypertrophy in vitro. Protein expression levels of (A and B) Col X, (A and C) Runx2, (A and D) BMPER, (A and E) BMP4 and (A and F) p-Smad1/Smad1. Immunofluorescent intensity of (G) Col X and (H) Runx2. *P

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