ABCG2 Antibody - #AF5177

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製品: ABCG2 Antibody
カタログ: AF5177
タンパク質の説明: Rabbit polyclonal antibody to ABCG2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Rabbit
分子量: 72 kDa; 72kD(Calculated).
ユニプロット: Q9UNQ0
RRID: AB_2837663

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Rabbit(89%)
クローナリティ:
Polyclonal
特異性:
ABCG2 Antibody detects endogenous levels of total ABCG2.
RRID:
AB_2837663
引用形式: Affinity Biosciences Cat# AF5177, RRID:AB_2837663.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ABC transporter; ABC15; ABCG 2; ABCG2; ABCG2_HUMAN; ABCP; ATP binding cassette sub family G (WHITE) member 2; ATP binding cassette transporter G2; ATP-binding cassette sub-family G member 2; BCRP; BCRP1; BMDP; Breast cancer resistance protein; CD338; CDw338; CDw338 antigen; EST157481; GOUT1; MGC102821; Mitoxantrone resistance associated protein; Mitoxantrone resistance-associated protein; MRX; Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2; MXR; MXR1; Placenta specific ATP binding cassette transporter; Placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter; UAQTL1;

免疫原

免疫原:
Uniprot:

Q9UNQ0(ABCG2_HUMAN) >>Visit HPA database.

遺伝子(ID):
ABCG2(9429)
発現特異性:
Q9UNQ0 ABCG2_HUMAN:

Highly expressed in placenta (PubMed:9850061). Low expression in small intestine, liver and colon (PubMed:9861027). Expressed in brain (at protein level) (PubMed:12958161).

タンパク質の説明:
High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain.
タンパク質配列:
MSSSNVEVFIPVSQGNTNGFPATASNDLKAFTEGAVLSFHNICYRVKLKSGFLPCRKPVEKEILSNINGIMKPGLNAILGPTGGGKSSLLDVLAARKDPSGLSGDVLINGAPRPANFKCNSGYVVQDDVVMGTLTVRENLQFSAALRLATTMTNHEKNERINRVIQELGLDKVADSKVGTQFIRGVSGGERKRTSIGMELITDPSILFLDEPTTGLDSSTANAVLLLLKRMSKQGRTIIFSIHQPRYSIFKLFDSLTLLASGRLMFHGPAQEALGYFESAGYHCEAYNNPADFFLDIINGDSTAVALNREEDFKATEIIEPSKQDKPLIEKLAEIYVNSSFYKETKAELHQLSGGEKKKKITVFKEISYTTSFCHQLRWVSKRSFKNLLGNPQASIAQIIVTVVLGLVIGAIYFGLKNDSTGIQNRAGVLFFLTTNQCFSSVSAVELFVVEKKLFIHEYISGYYRVSSYFLGKLLSDLLPMRMLPSIIFTCIVYFMLGLKPKADAFFVMMFTLMMVAYSASSMALAIAAGQSVVSVATLLMTICFVFMMIFSGLLVNLTTIASWLSWLQYFSIPRYGFTALQHNEFLGQNFCPGLNATGNNPCNYATCTGEEYLVKQGIDLSPWGLWKNHVALACMIVIFLTIAYLKLLFLKKYS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
89
Bovine
78
Sheep
78
Pig
75
Horse
67
Xenopus
56
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UNQ0 基板として

Site PTM Type Enzyme
S50 Phosphorylation
S100 Phosphorylation
S103 Phosphorylation
S143 Phosphorylation
K172 Ubiquitination
S187 Phosphorylation
T194 Phosphorylation
S195 Phosphorylation
K323 Ubiquitination
S353 Phosphorylation
K357 Ubiquitination
T362 Phosphorylation P11309 (PIM1)
Y464 Phosphorylation
N596 N-Glycosylation

研究背景

機能:

Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells. Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme. Also mediates the efflux of sphingosine-1-P from cells. Acts as a urate exporter functioning in both renal and extrarenal urate excretion. In kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate (By similarity). Also involved in the excretion of steroids like estrone 3-sulfate/E1S, 3beta-sulfooxy-androst-5-en-17-one/DHEAS, and other sulfate conjugates. Mediates the secretion of the riboflavin and biotin vitamins into milk (By similarity). Extrudes pheophorbide a, a phototoxic porphyrin catabolite of chlorophyll, reducing its bioavailability (By similarity). Plays an important role in the exclusion of xenobiotics from the brain (Probable). It confers to cells a resistance to multiple drugs and other xenobiotics including mitoxantrone, pheophorbide, camptothecin, methotrexate, azidothymidine, and the anthracyclines daunorubicin and doxorubicin, through the control of their efflux. In placenta, it limits the penetration of drugs from the maternal plasma into the fetus (By similarity). May play a role in early stem cell self-renewal by blocking differentiation (By similarity).

PTMs:

N-glycosylated. Glycosylation-deficient ABCG2 is normally expressed and functional.

Phosphorylated. Phosphorylation at Thr-362 by PIM1 is induced by drugs like mitoxantrone and is associated with cells increased drug resistance. It regulates the localization to the plasma membrane, the homooligomerization and therefore, the activity of the transporter.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein. Mitochondrion membrane>Multi-pass membrane protein.
Note: Enriched in membrane lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in placenta. Low expression in small intestine, liver and colon. Expressed in brain (at protein level).

サブユニット構造:

Homodimer; disulfide-linked. The minimal functional unit is a homodimer, but the major oligomeric form in plasma membrane is a homotetramer with possibility of higher order oligomerization up to homododecamers.

タンパク質ファミリー:

The extracellular loop 3 (ECL3) is involved in binding porphyrins and transfer them to other carriers, probably albumin.

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

研究領域

· Environmental Information Processing > Membrane transport > ABC transporters.

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

参考文献

1). Caffeic acid phenethyl ester alleviated hypouricemia in hyperuricemic mice through inhibiting XOD and up-regulating OAT3. PHYTOMEDICINE, 2022 (PubMed: 35714456) [IF=7.9]
2). Aqueous extract of Phellinus igniarius ameliorates hyperuricemia and renal injury in adenine/potassium oxonate-treated mice. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38879892) [IF=7.5]
3). MiR-21 modulates the polarization of M2 macrophages and increases the effects of M2 macrophages on promoting the chemoresistance of ovarian cancer. LIFE SCIENCES, 2020 (PubMed: 31837336) [IF=6.1]

Application: WB    Species: human    Sample: ovarian cancer cells

Figure 2. |Characterization of M0, M1 and M2 macrophages. M2 macrophages could promote the chemoresistance of ovarian cancer cells.(F) Western blotting was used to test the expression levels of cleaved caspase 3, ABCG2 and MDR1 in the ovarian cancer cells which co-cultured with M0 and M2 macrophages. U6 and GAPDH were used as the control. The columns showed as the mean ± SE of triplicate samples. *P < 0.05; **P < 0.01; ***P < 0.001.
4). Whey Protein Peptide Pro-Glu-Trp Ameliorates Hyperuricemia by Enhancing Intestinal Uric Acid Excretion, Modulating the Gut Microbiota, and Protecting the Intestinal Barrier in Rats. Journal of agricultural and food chemistry, 2024 (PubMed: 38240209) [IF=6.1]
5). Chinese Sumac (Rhus chinensis Mill.) Fruits Prevent Hyperuricemia and Uric Acid Nephropathy in Mice Fed a High-Purine Yeast Diet. Nutrients, 2024 (PubMed: 38257077) [IF=5.9]

Application: WB    Species: Mouse    Sample:

Figure 6 Effect of CE on the expression (A) and relative expressions (B) of several key proteins related to uric acid transport in the kidney, including ABCG2, URAT1, and SLC2A9 (n = 6/group). The relative expression level was quantified to 1 as the ratio of the blank group to β-actin as a standard for comparison. Different letters on the same bar graph indicate significant differences (p < 0.05). C, control group; M, model group; P, positive group (5 mg/kg b.w. of allopurinol). YL, CE low dose group (400 mg/kg b.w. of CE) and YH, CE high dose group (800 mg/kg b.w. of CE).
6). RPF2 mediates the CARM1‑MYCN axis to promote chemotherapy resistance in colorectal cancer cells. Oncology reports, 2024 (PubMed: 37997821) [IF=4.2]
7). Hypouricaemic and nephroprotective effects of Poria cocos in hyperuricemic mice by up-regulating ATP-binding cassette super-family G member 2. PHARMACEUTICAL BIOLOGY, 2021 (PubMed: 33651969) [IF=3.8]

Application: WB    Species: Mice    Sample: kidney tissues

Figure 4. Effects of PCE and PCW on renal ABCG2, OAT3, OAT1 and OCT2 protein expression detected by Western blot: immunoreactive bands (a) and densitometries (b,c,d and –e, expressed as mean ± SD; n n ¼ 3).  p < 0.05,  p < 0.01 versus the normal control; #p < 0.05, ##p < 0.01 versus the hyperuricemic control;  p < 0.01 versus the allopurinol control.
8). IFN‑γ induces apoptosis in gemcitabine‑resistant pancreatic cancer cells. Molecular medicine reports, 2024 (PubMed: 38488034) [IF=3.4]

Application: WB    Species: Human    Sample: PANC-1 and PANC-1/GEM cells

Figure 3. Assessment of MRP and BCRP expression in PANC-1 and PANC-1/GEM cells. (A) Reverse transcription-quantitative PCR analysis of MRP and BCRP mRNA expression levels in PANC-1 and PANC-1/GEM cells (n=3). (B) Western blot analysis of MRP and BCRP protein expression in PANC-1 and PANC-1/GEM cells (n=3). Data are presented as the mean ± SD. *P
9). Curcuma zedoaria petroleum ether extract reverses the resistance of triple-negative breast cancer to docetaxel via pregnane X receptor. Annals of Translational Medicine, 2021 (PubMed: 34733941)

Application: WB    Species: Mice    Sample: MDA-MB-231/docetaxel cells

Figure 3 Effect of PECZ on the protein expression of drug resistance genes in MDA-MB-231/docetaxel cells. Representative band of each protein (A). Relative protein expression of PXR (B), MDR1 (C), BCRP (D), and CYP3A4 (E). (x¯±s, n=3), *, P<0.05; **, P<0.01 vs. control group. PECZ, petroleum ether extracts of Curcuma zedoaria; PXR, pregnane X receptor; MDR1, multidrug resistance 1; BCRP, breast cancer resistance protein; CYP3A4, cytochrome P-450.

Application: IHC    Species: Mice    Sample: tumor tissues

Figure 7 PECZ and docetaxel decreased the expression of drug resistance genes. Representative images of immunohistochemical showing PXR, MDR1, CYP3A4, and BCRP in tumor tissues in the different groups. Original magnification ×200, ×400; (x¯±s, n=3). PECZ, petroleum ether extracts of Curcuma zedoaria; PXR, pregnane X receptor; MDR1, multidrug resistance 1; BCRP, breast cancer resistance protein; CYP3A4, cytochrome P-450.
10). IL-17A exacerbates cisplatin-based resistance of OVCA via upregulating the expression of ABCG2 and MDR1 through Gli1-mediated Hh signaling. Oncotarget, 2016

Application: WB    Species: Human    Sample: A2780

Figure 4: Gli1-mediated Hh signal pathway is involved in the enhancing effect of rhIL-17A on DDP-based resistance of OVCA cells. A. Up-regulating effects of rhIL-17A on the levels of ABCG2, MDR1 and Gli1 in A2780 and OVCAR3 cells could be abolished by neutralizing IL-17RA mAb or IL-17RA knockdown. OVCA cells were pretreated with 3μg/ml neutralizing IL-17RA mAb for 1h (a) or transient silenced by siRNA targeting IL-17RA for 48h (b), and then treated with 1ng/ml rhIL-17A for 24h. B. Up-regulating effects of rhIL-17A on the expression of ABCG2, MDR1 and Gli1 in A2780 and OVCAR3 cells could be blocked by Gant61. OVCA cells were pretreated with 25μM Gant 61 for 1h and then treated with 1ng/ml rhIL-17A for 24h. Protein lysates from OVCA cells were prepared and analyzed by western blotting. Representative result from three independent experiments was showed. C. Gant61 could partially reverse the increase-effect of rhIL-17A on OVCA cell viability. A2780 and OVCAR3 cells were pretreated with 25μM Gant 61 for 1h and then treated with 1ng/ml rhIL-17A and/or DDP (10μM for A2780 cells, 100μM for OVCAR3 cells) for 24h. Cell viability was detected by MTT assay. Data represent means±SD from three independent experiments.
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