EPO Antibody - #AF5190

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製品: EPO Antibody
カタログ: AF5190
タンパク質の説明: Rabbit polyclonal antibody to EPO
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 21 kDa; 21kD(Calculated).
ユニプロット: P01588
RRID: AB_2837676

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(80%), Bovine(80%), Horse(80%), Sheep(80%), Rabbit(90%), Dog(80%)
クローナリティ:
Polyclonal
特異性:
EPO Antibody detects endogenous levels of total EPO.
RRID:
AB_2837676
引用形式: Affinity Biosciences Cat# AF5190, RRID:AB_2837676.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

EP; EPO alpha; EPO; Epoetin; Erythropoetin; Erythropoietin precursor; MVCD2;

免疫原

免疫原:
Uniprot:

P01588(EPO_HUMAN) >>Visit HPA database.

遺伝子(ID):
EPO(2056)
発現特異性:
P01588 EPO_HUMAN:

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

タンパク質の説明:
Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
タンパク質配列:
MGVHECPAWLWLLLSLLSLPLGLPVLGAPPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
90
Pig
80
Horse
80
Bovine
80
Sheep
80
Dog
80
Xenopus
67
Chicken
50
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01588 基板として

Site PTM Type Enzyme
S111 Phosphorylation
S127 Phosphorylation
S131 Phosphorylation
S153 O-Glycosylation
T161 Phosphorylation

研究背景

機能:

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

タンパク質ファミリー:

Belongs to the EPO/TPO family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

参考文献

1). Case Report: Myomatous erythrocytosis syndrome presenting as rapid growth of an extra-uterine mass. Frontiers in Surgery, 2022 (PubMed: 35983553) [IF=1.8]

Application: IHC    Species: Human    Sample: myomatous tissue

Figure 2 (A–D): Immunohistochemical staining revealed excessive Epo and Epo-R expression of the broad ligament myoma. Immunochemical staining showed diffuse (>50%) Epo expression with moderate to strong staining intensity over the myomatous cells (A, 40×), whereas the endothelial cells were negatively stained (B, 200×). A diffuse (>50%) Epo-R expression with moderate staining intensity was found over the myomatous cells (C, 40×). The endothelial cells also presented with diffuse, moderate to weak staining intensity (D, 200×). EPO, erythropoietin; Epo-R, erythropoietin receptor.

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