ACE1 Antibody - #AF5197

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製品: ACE1 Antibody
カタログ: AF5197
タンパク質の説明: Rabbit polyclonal antibody to ACE1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 149 kDa; 150kD(Calculated).
ユニプロット: P12821
RRID: AB_2837683

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%)
クローナリティ:
Polyclonal
特異性:
ACE1 Antibody detects endogenous levels of total ACE1.
RRID:
AB_2837683
引用形式: Affinity Biosciences Cat# AF5197, RRID:AB_2837683.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ACE 1; ACE; ACE T; ACE_HUMAN; ACE1; Angiotensin converting enzyme somatic isoform; Angiotensin converting enzyme testis specific isoform; Angiotensin I converting enzyme 1; Angiotensin I converting enzyme; Angiotensin I converting enzyme peptidyl dipeptidase A 1; angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript; Angiotensin-converting enzyme; Carboxycathepsin; CD 143; CD143; CD143 antigen; DCP 1; DCP; DCP1; Dipeptidyl carboxypeptidase 1; Dipeptidyl carboxypeptidase I; Kininase II; MGC26566; MVCD3; Peptidase P; Peptidyl dipeptidase A; soluble form; Testicular ECA;

免疫原

免疫原:
Uniprot:

P12821(ACE_HUMAN) >>Visit HPA database.

遺伝子(ID):
ACE(1636)
発現特異性:
P12821 ACE_HUMAN:

Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.

タンパク質の説明:
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
タンパク質配列:
MGAASGRRGPGLLLPLPLLLLLPPQPALALDPGLQPGNFSADEAGAQLFAQSYNSSAEQVLFQSVAASWAHDTNITAENARRQEEAALLSQEFAEAWGQKAKELYEPIWQNFTDPQLRRIIGAVRTLGSANLPLAKRQQYNALLSNMSRIYSTAKVCLPNKTATCWSLDPDLTNILASSRSYAMLLFAWEGWHNAAGIPLKPLYEDFTALSNEAYKQDGFTDTGAYWRSWYNSPTFEDDLEHLYQQLEPLYLNLHAFVRRALHRRYGDRYINLRGPIPAHLLGDMWAQSWENIYDMVVPFPDKPNLDVTSTMLQQGWNATHMFRVAEEFFTSLELSPMPPEFWEGSMLEKPADGREVVCHASAWDFYNRKDFRIKQCTRVTMDQLSTVHHEMGHIQYYLQYKDLPVSLRRGANPGFHEAIGDVLALSVSTPEHLHKIGLLDRVTNDTESDINYLLKMALEKIAFLPFGYLVDQWRWGVFSGRTPPSRYNFDWWYLRTKYQGICPPVTRNETHFDAGAKFHVPNVTPYIRYFVSFVLQFQFHEALCKEAGYEGPLHQCDIYRSTKAGAKLRKVLQAGSSRPWQEVLKDMVGLDALDAQPLLKYFQPVTQWLQEQNQQNGEVLGWPEYQWHPPLPDNYPEGIDLVTDEAEASKFVEEYDRTSQVVWNEYAEANWNYNTNITTETSKILLQKNMQIANHTLKYGTQARKFDVNQLQNTTIKRIIKKVQDLERAALPAQELEEYNKILLDMETTYSVATVCHPNGSCLQLEPDLTNVMATSRKYEDLLWAWEGWRDKAGRAILQFYPKYVELINQAARLNGYVDAGDSWRSMYETPSLEQDLERLFQELQPLYLNLHAYVRRALHRHYGAQHINLEGPIPAHLLGNMWAQTWSNIYDLVVPFPSAPSMDTTEAMLKQGWTPRRMFKEADDFFTSLGLLPVPPEFWNKSMLEKPTDGREVVCHASAWDFYNGKDFRIKQCTTVNLEDLVVAHHEMGHIQYFMQYKDLPVALREGANPGFHEAIGDVLALSVSTPKHLHSLNLLSSEGGSDEHDINFLMKMALDKIAFIPFSYLVDQWRWRVFDGSITKENYNQEWWSLRLKYQGLCPPVPRTQGDFDPGAKFHIPSSVPYIRYFVSFIIQFQFHEALCQAAGHTGPLHKCDIYQSKEAGQRLATAMKLGFSRPWPEAMQLITGQPNMSASAMLSYFKPLLDWLRTENELHGEKLGWPQYNWTPNSARSEGPLPDSGRVSFLGLDLDAQQARVGQWLLLFLGIALLVATLGLSQRLFSIRHRSLHRHSHGPQFGSEVELRHS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Chicken
86
Bovine
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12821 基板として

Site PTM Type Enzyme
N38 N-Glycosylation
N54 N-Glycosylation
N74 N-Glycosylation
N111 N-Glycosylation
K136 Acetylation
Y140 Phosphorylation
N146 N-Glycosylation
Y151 Phosphorylation
N318 N-Glycosylation
N445 N-Glycosylation
N509 N-Glycosylation
N695 N-Glycosylation
T697 Phosphorylation
N714 N-Glycosylation
T716 Phosphorylation
K718 Ubiquitination
N760 N-Glycosylation
R814 Methylation
S824 Phosphorylation
R826 Methylation
N942 N-Glycosylation
N1191 N-Glycosylation
S1299 Phosphorylation P68400 (CSNK2A1)

研究背景

機能:

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.

PTMs:

Phosphorylated by CK2 on Ser-1299; which allows membrane retention.

細胞の位置付け:

Secreted.

Cell membrane>Single-pass type I membrane protein. Cytoplasm.
Note: Detected in both cell membrane and cytoplasm in neurons.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.

タンパク質ファミリー:

Belongs to the peptidase M2 family.

研究領域

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Organismal Systems > Endocrine system > Renin-angiotensin system.   (View pathway)

· Organismal Systems > Endocrine system > Renin secretion.

参考文献

1). GTS-21 attenuates ACE/ACE2 ratio and glycocalyx shedding in lipopolysaccharide-induced acute lung injury by targeting macrophage polarization derived ADAM-17. International immunopharmacology, 2024 (PubMed: 38310766) [IF=5.6]

Application: WB    Species: Mouse    Sample: lung tissue

Fig. 2. GTS-21 regulated the ACE/ACE2 ratio and glycocalyx shedding in LPS-ALI mice A-D ELISA kits were used to quantify the concentrations of major renin-angiotensin system (RAS) regulating enzymes (ACE, Ang II, ACE2, and Ang 1-7) in the lung tissue (n = 6 per group). E Protein levels of important RAS-regulating enzymes (ACE, AT1R, ACE2, and MasR) (n = 3 per group). The relative protein levels are displayed. F Levels of NF-κB phosphoproteins (p-P65 and p-IκB) (n = 3 per group). The relative protein levels are displayed. The immunofluorescence of HS, SDC-1, and surfactant protein D in the lungs is shown in G in representative images and densitometry (scar bar, 20 mm, n = 3 per group). H Evans Blue staining tested lung permeability (n = 3 for each group). Using ELISA, we assessed the BALF concentrations of HS I and HPA J; SP-D in serum K and BALF L (n = 5 for each group). M Relative protein levels of SDC-1, HPA, EXT-1, and SP-D in The lung tissues (n = 3 for each group). All data were shown as mean ± SD.

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