ESA Antibody - #AF5207
(1)
(6)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5207, RRID:AB_2837693.
折りたたみ／展開
ECS-1; ECS1; Epidermal surface antigen 1; Epidermal surface antigen; ESA; ESA1; Flot2; FLOT2_HUMAN; Flotillin 2 (epidermal surface antigen 1); Flotillin-2; M17S1; Membrane component chromosome 17 surface marker 1; Membrane component chromosome 17 surface marker 1 homolog; membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1); Membrane component, chromosome 17, surface marker 1; REG-1; Reggie-1; Reggie-2;
免疫原
In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
- Q14254 FLOT2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGNCHTVGPNEALVVSGGCCGSDYKQYVFGGWAWAWWCISDTQRISLEIMTLQPRCEDVETAEGVALTVTGVAQVKIMTEKELLAVACEQFLGKNVQDIKNVVLQTLEGHLRSILGTLTVEQIYQDRDQFAKLVREVAAPDVGRMGIEILSFTIKDVYDKVDYLSSLGKTQTAVVQRDADIGVAEAERDAGIREAECKKEMLDVKFMADTKIADSKRAFELQKSAFSEEVNIKTAEAQLAYELQGAREQQKIRQEEIEIEVVQRKKQIAVEAQEILRTDKELIATVRRPAEAEAHRIQQIAEGEKVKQVLLAQAEAEKIRKIGEAEAAVIEAMGKAEAERMKLKAEAYQKYGDAAKMALVLEALPQIAAKIAAPLTKVDEIVVLSGDNSKVTSEVNRLLAELPASVHALTGVDLSKIPLIKKATGVQV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14254 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| R127 | Methylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| Y163 | Phosphorylation | P06241 (FYN) | Uniprot |
| S166 | Phosphorylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| T285 | Phosphorylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| Y348 | Phosphorylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| Y351 | Phosphorylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| T392 | Phosphorylation | Uniprot | |
| S405 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot |
研究背景
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
ZDHHC5-catalyzed palmitoylation predominantly occurs at Cys-4. ZDHHC5-catalyzed palmitoylation may be required for the formation of higher-order complexes and for neurite outgrowth in cultured neural stem cells.
Cell membrane>Peripheral membrane protein. Membrane>Caveola>Peripheral membrane protein. Endosome. Membrane>Lipid-anchor.
Note: Membrane-associated protein of caveolae.
In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity). Interacts with ECPAS.
Belongs to the band 7/mec-2 family. Flotillin subfamily.
研究領域
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
参考文献
Application: WB Species: Hu Sheep Sample: liver tissue
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