HMGA1 Antibody - #AF5218
(3)
(4)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5218, RRID:AB_2837704.
折りたたみ／展開
High mobility group (nonhistone chromosomal) protein isoforms I and Y; High mobility group AT hook 1; High mobility group AT-hook protein 1; High mobility group protein 1; High mobility group protein A1; High mobility group protein HMG-I/HMG-Y; High mobility group protein Ia; High mobility group protein R; HMG R; HMG-I(Y); HMGA1; HMGA1_HUMAN; HMGA1A; HMGIY; MGC12816; MGC4242; MGC4854; Nonhistone chromosomal high mobility group protein HMG I/HMG Y;
免疫原
- P17096 HMGA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P17096 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| K7 | Acetylation | Uniprot | |
| K7 | Methylation | Uniprot | |
| K7 | Ubiquitination | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K15 | Acetylation | Uniprot | |
| K15 | Methylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| K18 | Acetylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| K23 | Acetylation | Uniprot | |
| K23 | Methylation | Uniprot | |
| R24 | Methylation | Uniprot | |
| R26 | Methylation | Uniprot | |
| K31 | Acetylation | Uniprot | |
| K31 | Methylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| S36 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) , Q9H2X6 (HIPK2) | Uniprot |
| T39 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| K46 | Acetylation | Uniprot | |
| K46 | Methylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| T53 | Phosphorylation | Q9H2X6 (HIPK2) , P06493 (CDK1) | Uniprot |
| K55 | Acetylation | Uniprot | |
| K55 | Methylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| R58 | Methylation | Uniprot | |
| R60 | Methylation | Uniprot | |
| K62 | Acetylation | Uniprot | |
| K62 | Methylation | Uniprot | |
| S64 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| K65 | Acetylation | Uniprot | |
| K65 | Methylation | Uniprot | |
| K65 | Sumoylation | Uniprot | |
| K67 | Acetylation | Uniprot | |
| K67 | Methylation | Uniprot | |
| K71 | Acetylation | Uniprot | |
| K71 | Methylation | Uniprot | |
| T72 | Phosphorylation | Uniprot | |
| R73 | Methylation | Uniprot | |
| K74 | Acetylation | Uniprot | |
| K74 | Methylation | Uniprot | |
| T75 | Phosphorylation | Uniprot | |
| T76 | Phosphorylation | Uniprot | |
| T77 | Phosphorylation | Uniprot | |
| T78 | Phosphorylation | P06493 (CDK1) , Q9H2X6 (HIPK2) | Uniprot |
| R81 | Methylation | Uniprot | |
| K82 | Acetylation | Uniprot | |
| R84 | Methylation | Uniprot | |
| R86 | Methylation | Uniprot | |
| K88 | Acetylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot |
研究背景
HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity.
HMG-Y is not methylated.
Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
Nucleus. Chromosome.
Interacts with HIPK2 (By similarity).
Belongs to the HMGA family.
参考文献
Application: WB Species: Human Sample:
Application: WB Species: Human Sample: OVCAR-3 cells
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