Cytokeratin 14 Antibody - #AF5225
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5225, RRID:AB_2837711.
折りたたみ／展開
CK 14; CK-14; ck14; Cytokeratin 14; Cytokeratin-14; Cytokeratin14; Dowling Meara; EBS3; EBS4; Epidermolysis bullosa simplex; K14; K1C14_HUMAN; Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); Keratin 14; Keratin; Keratin type I cytoskeletal 14; Keratin, type I cytoskeletal 14; Keratin-14; Keratin14; Koebner; Krt 14; Krt14; NFJ; OTTHUMP00000164624; type I cytoskeletal 14;
免疫原
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
- P02533 K1C14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSSRFSSGGACGLGGGYGGGFSSSSSSFGSGFGGGYGGGLGAGLGGGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPAEIKDYSPYFKTIEDLRNKILTATVDNANVLLQIDNARLAADDFRTKYETELNLRMSVEADINGLRRVLDELTLARADLEMQIESLKEELAYLKKNHEEEMNALRGQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P02533 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T9 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| K15 | Methylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| T45 | Phosphorylation | Uniprot | |
| Y46 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| Y129 | Phosphorylation | Uniprot | |
| K132 | Acetylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| Y151 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| Y165 | Phosphorylation | Uniprot | |
| Y204 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| K243 | Methylation | Uniprot | |
| Y248 | Phosphorylation | Uniprot | |
| K250 | Sumoylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| Y291 | Phosphorylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| T319 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| Y398 | Phosphorylation | Uniprot | |
| K399 | Ubiquitination | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| T414 | Phosphorylation | Uniprot | |
| Y415 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot |
研究背景
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.
Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.
Cytoplasm. Nucleus.
Note: Expressed in both as a filamentous pattern.
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
Heterotetramer of two type I and two type II keratins. disulfide-linked keratin-14 associates with keratin-5. Interacts with TRADD and with keratin filaments. Associates with other type I keratins. Interacts with EPPK1 (By similarity). Interacts with KLHL24.
Belongs to the intermediate filament family.
研究領域
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
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