hnRNP H Antibody - #AF5241
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5241, RRID:AB_2837727.
折りたたみ／展開
DKFZp686A15170; Heterogeneous nuclear ribonucleoprotein H; Heterogeneous nuclear ribonucleoprotein H1 (H); Heterogeneous nuclear ribonucleoprotein H1; HNRH1_HUMAN; hnRNP H; hnRNPH; Hnrnph1; HNRPH 1; HNRPH; HNRPH1 protein; N-terminally processed;
免疫原
- P31943 HNRH1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMLGTEGGEGFVVKVRGLPWSCSADEVQRFFSDCKIQNGAQGIRFIYTREGRPSGEAFVELESEDEVKLALKKDRETMGHRYVEVFKSNNVEMDWVLKHTGPNSPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P31943 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| M2 | Acetylation | Uniprot | |
| T5 | Phosphorylation | Uniprot | |
| K14 | Sumoylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| R29 | Methylation | Uniprot | |
| C34 | S-Nitrosylation | Uniprot | |
| K35 | Sumoylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| R44 | Methylation | Uniprot | |
| Y47 | Phosphorylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| K68 | Sumoylation | Uniprot | |
| K72 | Sumoylation | Uniprot | |
| R81 | Methylation | Uniprot | |
| K87 | Sumoylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| K98 | Acetylation | Uniprot | |
| K98 | Sumoylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| T100 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | P28482 (MAPK1) , P24941 (CDK2) | Uniprot |
| T107 | Phosphorylation | Uniprot | |
| R114 | Methylation | Uniprot | |
| C122 | S-Nitrosylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| T152 | O-Glycosylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| K167 | Acetylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| Y180 | Phosphorylation | Uniprot | |
| K185 | Acetylation | Uniprot | |
| K185 | Methylation | Uniprot | |
| K185 | Sumoylation | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| Y195 | Phosphorylation | Uniprot | |
| R199 | Methylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| R206 | Methylation | Uniprot | |
| Y210 | Phosphorylation | Uniprot | |
| R212 | Methylation | Uniprot | |
| R217 | Methylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| R224 | Methylation | Uniprot | |
| R230 | Methylation | Uniprot | |
| R232 | Methylation | Uniprot | |
| R233 | Methylation | Uniprot | |
| Y236 | Phosphorylation | Uniprot | |
| Y240 | Phosphorylation | Uniprot | |
| Y243 | Phosphorylation | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| Y249 | Phosphorylation | Uniprot | |
| Y253 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| R259 | Methylation | Uniprot | |
| R262 | Methylation | Uniprot | |
| Y266 | Phosphorylation | Uniprot | |
| C267 | S-Nitrosylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| Y276 | Phosphorylation | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| R294 | Methylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| Y306 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| T328 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| Y372 | Phosphorylation | Uniprot | |
| Y376 | Phosphorylation | Uniprot |
研究背景
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).
Nucleus>Nucleoplasm.
Expressed ubiquitously.
Part of a ternary complex containing FUBP2, PTBP1, PTBP2 and HNRNPH1. Identified in the spliceosome C complex. Interacts with IGF2BP1. Interacts with CUGBP1; the interaction is RNA-dependent. Interacts with MBNL1; the interaction in RNA-independent.
Each quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA).
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