Cleaved-Caspase 9 (Asp330) Antibody - #AF5244

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製品: Cleaved-Caspase 9 (Asp330) Antibody
カタログ: AF5244
タンパク質の説明: Rabbit polyclonal antibody to Cleaved-Caspase 9 (Asp330)
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human
分子量: 10 kDa; 46kD(Calculated).
ユニプロット: P55211
RRID: AB_2837730

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
Cleaved-Caspase 9 (Asp330) Antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp330.
RRID:
AB_2837730
引用形式: Affinity Biosciences Cat# AF5244, RRID:AB_2837730.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

APAF-3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP-9; CASP9; CASP9_HUMAN; Caspase 9 apoptosis related cysteine peptidase; Caspase 9 Dominant Negative; Caspase 9c; Caspase-9; Caspase-9 subunit p10; ICE LAP6; ICE like apoptotic protease 6; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1, regulatory subunit 56; RNCASP9;

免疫原

免疫原:

A synthesized peptide derived from human Caspase 9 (Cleaved-Asp330).

Uniprot:

P55211(CASP9_HUMAN) >>Visit HPA database.

遺伝子(ID):
CASP9(842)
発現特異性:
P55211 CASP9_HUMAN:

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

タンパク質の説明:
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
タンパク質配列:
MDEADRRLLRRCRLRLVEELQVDQLWDALLSRELFRPHMIEDIQRAGSGSRRDQARQLIIDLETRGSQALPLFISCLEDTGQDMLASFLRTNRQAAKLSKPTLENLTPVVLRPEIRKPEVLRPETPRPVDIGSGGFGDVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS

研究背景

機能:

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).

Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

PTMs:

Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.

Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.

組織特異性:

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

タンパク質ファミリー:

Belongs to the peptidase C14A family.

研究領域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

参考文献

1). CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma. Molecular Cancer, 2022 (PubMed: 35858900) [IF=37.3]

Application: WB    Species: Human    Sample: HepG2 and Hep3B cells

Fig. 6 FTO is regulated by circGPR137B/miR-4739 axis in HCC. A Schematic representation of the binding sites between miR-4739 and FTO 3’UTR. B Comparison of the luciferase activity of WT or Mut FTO 3’UTR after treatment with miR-4739 mimics in HepG2 and Hep3B cells. C, D RT-qPCR and western blot analysis of the expression of FTO, p21, p27, cleaved caspase-3/− 9, N-cadherin, E-cadherin and vimentin after the transfection with circGPR137B lentiviruses and (or) miR-4739 mimics in HepG2 and Hep3B cells. E, F, G MTT, colony formation and transwell analysis of the cell proliferation, colony number and cell invasion after the transfection with FTO plasmids and (or) miR-4739 mimics in HepG2 and Hep3B cells. Data are the means ± SEM of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001
2). Cytotoxicity of adducts formed between quercetin and methylglyoxal in PC-12 cells. Food Chemistry, 2021 (PubMed: 33706136) [IF=8.5]

Application: WB    Species: Rat    Sample: PC-12 cells

Fig. 5. Effect of treatments of MGO, Que-mono-MGO, and Que-di-MGO on the expression levels of apoptotic markers and components of AKT and Nrf2-HO-1/NQO-1 signaling pathways. Significant differences (p < 0.05) between samples of different treatments are marked with different letters on each column.
3). Targeting TNFAIP2 with NIR-II CRISPR-Cas9 nanosystem to overcome cisplatin resistance in laryngeal cancer. NPJ precision oncology, 2025 (PubMed: 40731142) [IF=7.9]

Application: WB    Species: human    Sample:

Fig. 4: Analysis of the impact of TNFAIP2 gene knockout on ROS production in cisplatin-induced apoptosis. A Mitochondrial Reactive Oxygen Species (mtROS) levels detected using MitoSOX dye (scale bar: 25 μm); B GSH and GSSG levels measured in cell lines using GSH and GSSG detection kits, with the GSH/GSSG ratio calculated; C SOD enzyme activity measured using a SOD activity assay kit; D POD enzyme activity measured using a POD activity assay kit; E CAT enzyme activity measured using a CAT activity assay kit; F, G Apoptosis in TNFAIP2 knockout cells induced by cisplatin, with and without NAC (4 mmol/L, pretreated for 2 h), analyzed by flow cytometry; H, I Expression levels of JNK protein and other related molecular markers in the apoptosis signaling pathway induced by cisplatin, with and without NAC (4 mmol/L, pretreated for 2 h), analyzed by Western blot in control and TNFAIP2 knockout cell lines; J Schematic representation of the ROS/JNK signaling pathway in cisplatin-induced apoptosis. *p 
4). BCAS1-positive immature oligodendrocytes are affected by the α-synuclein-induced pathology of multiple system atrophy. Acta neuropathologica communications, 2020 (PubMed: 32727582) [IF=6.2]

Application: IF/ICC    Species: Rat    Sample:

Fig. 4 α-Syn PFF exposure to immature OLGs during differentiation-induced abnormal oligodendrogenesis. a Confocal images of oligodendroglial cells on day 4 after differentiation induction showing the colocalization of α-syn immunoreactivity and cleaved caspase-9 expression. The cells were incubated with 1 μM α-syn PFFs for 24 h (day 3–4) before fixation. White arrowheads indicate intracellular inclusions. The regions marked by dotted squares are highlighted in the magnified views. Scale bar = 5 μm. b Scheme showing the experimental protocols of 3 μM α-syn PFF exposure to oligodendroglial cells during two different phases (day 0–1, or day 3–4) of differentiation. c Cell viability analysis (WST assay) of day 8 OLGs, which were obtained through different protocols of α-syn PFF exposure during differentiation (no exposure, exposure on day 0–1, or exposure on days 3–4). N = 5, respectively, independent culture, Kruskal–Wallis, p* 
5). Photoprotective Effects of Dendrobium nobile Lindl. Polysaccharides against UVB-Induced Oxidative Stress and Apoptosis in HaCaT Cells. International Journal of Molecular Sciences, 2023 (PubMed: 37047098) [IF=5.6]

Application: IF/ICC    Species: Human    Sample: HaCaT cells

Figure 8 DNPs inhibit UVB-induced apoptosis-related proteins expression in HaCaT cells. (A) Representative fluorescence images showing cleaved caspase-9 staining and (B) cleaved caspase-3 staining. Scale bar = 100 μm (A)/20 μm (B); Cleaved caspase-9 and cleaved caspase-3 positive cells were counted (C,D). ### p < 0.001 vs. control;
6). HOXB4/METTL7B cascade mediates malignant phenotypes of hepatocellular carcinoma through TKT m6A modification. Biology direct, 2025 (PubMed: 40045399) [IF=5.5]

Application: WB    Species: human    Sample: Li-7 cells

Fig. 2 HOXB4 inhibits proliferation and promotes apoptosis of hepatocellular carcinoma cell in vitro. (a-b) The proliferation of HCC cells with upregulated or downregulated HOXB4 expression; (c-d) EdU assay detected the proliferation of HCC cells with upregulated or downregulated HOXB4 expression. Scale bar, 50 μm; (e-f) Colony formation ability of HCC cells with upregulated or downregulated HOXB4 expression; (g) Representative immunoblots of PCNA in Li-7 cells with upregulated or downregulated HOXB4 expression; (h) Cell apoptosis of Li-7 cells with upregulated HOXB4 expression according to flow cytometry analysis; (i) Representative immunoblots of Bax, Bcl-2, cleaved caspase 3, and cleaved caspase 9 protein levels in Li-7 cells with upregulated HOXB4 expression. Data are expressed as mean ± SD, N = 3. **p 
7). Discovery of petroleum ether extract of eclipta targeting p53/Fas pathway for the treatment of chemotherapy-induced alopecia: Network pharmacology and experimental validation. Journal of ethnopharmacology, 2024 (PubMed: 38844249) [IF=4.8]
8). Lutein inhibits glutamate-induced apoptosis in HT22 cells via the Nrf2/HO-1 signaling pathway. Frontiers in neuroscience, 2024 (PubMed: 39193525) [IF=4.3]
9). ADAM28 from both endothelium and gastric cancer cleaves von Willebrand Factor to eliminate von Willebrand Factor-induced apoptosis of gastric cancer cells. European Journal of Pharmacology, 2021 (PubMed: 33675784) [IF=4.2]

Application: WB    Species: Human    Sample: SGC7901 cells

Fig. 5. In the co-culture system, ADAM28 derived from endothelium reduces the apoptosis of lower ventricular gastric cancer cells. (A) Western blot analysis of ADAM28 expression in HUVECs with ADAM28 overexpression or knockdown (***P < 0.001). (B–C) Flow cytometry was used to analyze the effects of HUVEC, HUVEC-ADAM28-KD and HUVEC-ADAM28-OE on the apoptosis of gastric cancer cells in the lower ventricle. Percentage of apoptotic cells compared to control was quantitated by mean fluorescence intensity and was shown in right graphs (***P < 0.001). (D) When HUVEC, HUVEC-ADAM28-KD and HUVEC-ADAM28-OE were added into the upper chamber, Western blot analysis was performed on SGC7901 cells in the lower chamber with antibodies against Casp3/c-Casp3 and Casp9/cCasp9. β-actin was used as a loading control. Each bar represented the mean ± S.D. of three independent experiments.
10). S14G-humanin alleviates acute lung injury by inhibiting the activation of NF-κB. Aging, 2023 (PubMed: 38054825) [IF=3.9]
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