BUB3 Antibody - #AF5252
| 製品: | BUB3 Antibody |
| カタログ: | AF5252 |
| タンパク質の説明: | Rabbit polyclonal antibody to BUB3 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 37 kDa; 37kD(Calculated). |
| ユニプロット: | O43684 |
| RRID: | AB_2837738 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5252, RRID:AB_2837738.
折りたたみ／展開
Aa2-050; AU019800; AU021329; AU043350; AW146323; BUB 3; BUB 3L; BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog; bub3; BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3 mitotic checkpoint protein; BUB3_HUMAN; BUB3L; Budding uninhibited by benomyl; Budding uninhibited by benzimidazoles 3; Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae); Budding uninhibited by benzimidazoles 3 homolog (yeast); Budding uninhibited by benzimidazoles 3 homolog; Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of; C78067; hBUB3; Mitotic checkpoint component; Mitotic checkpoint protein bub3; OTTHUMP00000020679; OTTHUMP00000020680; WD repeat type I transmembrane protein A72.5;
免疫原
- O43684 BUB3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43684 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Sumoylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| S18 | Phosphorylation | PR:O43683 (hBUB1) | Uniprot |
| S19 | Phosphorylation | O43683 (BUB1) | Uniprot |
| K21 | Ubiquitination | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| R126 | Methylation | Uniprot | |
| T127 | Phosphorylation | Uniprot | |
| C129 | S-Nitrosylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| R149 | Methylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| K179 | Acetylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| R186 | Methylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| Y194 | Phosphorylation | Uniprot | |
| Y207 | Phosphorylation | P14618 (PKM) | Uniprot |
| S211 | Phosphorylation | Uniprot | |
| K216 | Acetylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K227 | Sumoylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K322 | Acetylation | Uniprot | |
| K322 | Sumoylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| C327 | S-Nitrosylation | Uniprot | |
| T328 | Phosphorylation | Uniprot |
研究背景
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Poly-ADP-ribosylated by PARP1.
Nucleus. Chromosome>Centromere>Kinetochore.
Note: Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3.
Belongs to the WD repeat BUB3 family.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
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