hnRNP L Antibody - #AF5260

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製品: hnRNP L Antibody
カタログ: AF5260
タンパク質の説明: Rabbit polyclonal antibody to hnRNP L
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
分子量: 60 kDa; 64kD(Calculated).
ユニプロット: P14866
RRID: AB_2837746

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(82%)
クローナリティ:
Polyclonal
特異性:
hnRNP L Antibody detects endogenous levels of total hnRNP L.
RRID:
AB_2837746
引用形式: Affinity Biosciences Cat# AF5260, RRID:AB_2837746.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

D830027H13Rik; FLJ35509; Heterogeneous nuclear ribonucleoprotein L; hnRNP L; hnRNP-L; Hnrnpl; hnRPL; HNRPL_HUMAN; P/OKcl.14;

免疫原

免疫原:
Uniprot:

P14866(HNRPL_HUMAN) >>Visit HPA database.

遺伝子(ID):
HNRNPL(3191)
タンパク質の説明:
Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
タンパク質配列:
MSRRLLPRAEKRRRRLEQRQQPDEQRRRSGAMVKMAAAGGGGGGGRYYGGGSEGGRAPKRLKTDNAGDQHGGGGGGGGGAGAAGGGGGGENYDDPHKTPASPVVHIRGLIDGVVEADLVEALQEFGPISYVVVMPKKRQALVEFEDVLGACNAVNYAADNQIYIAGHPAFVNYSTSQKISRPGDSDDSRSVNSVLLFTILNPIYSITTDVLYTICNPCGPVQRIVIFRKNGVQAMVEFDSVQSAQRAKASLNGADIYSGCCTLKIEYAKPTRLNVFKNDQDTWDYTNPNLSGQGDPGSNPNKRQRQPPLLGDHPAEYGGPHGGYHSHYHDEGYGPPPPHYEGRRMGPPVGGHRRGPSRYGPQYGHPPPPPPPPEYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKLNVCVSKQPAIMPGQSYGLEDGSCSYKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPSSVKVFSGKSERSSSGLLEWESKSDALETLGFLNHYQMKNPNGPYPYTLKLCFSTAQHAS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
82
Zebrafish
78
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14866 基板として

Site PTM Type Enzyme
S29 Phosphorylation
K34 Acetylation
K34 Ubiquitination
R46 Methylation
Y47 Phosphorylation
Y48 Phosphorylation
S52 Phosphorylation
R56 Methylation
K59 Acetylation
K59 Sumoylation
K59 Ubiquitination
K62 Sumoylation
K62 Ubiquitination
T63 Phosphorylation
Y92 Phosphorylation
K97 Acetylation
K97 Ubiquitination
T98 Phosphorylation
S101 Phosphorylation
R107 Methylation
K136 Sumoylation
S185 Phosphorylation
S188 Phosphorylation
K229 Ubiquitination
K248 Ubiquitination
S250 Phosphorylation
S258 Phosphorylation
C260 S-Nitrosylation
C261 S-Nitrosylation
K264 Acetylation
K264 Ubiquitination
Y267 Phosphorylation
K269 Acetylation
K269 Ubiquitination
K277 Sumoylation
K277 Ubiquitination
T282 Phosphorylation
Y285 Phosphorylation
S291 Phosphorylation
S298 Phosphorylation
K302 Acetylation
K302 Sumoylation
K302 Ubiquitination
Y328 Phosphorylation
Y333 Phosphorylation
Y340 Phosphorylation
R344 Methylation
R354 Methylation
R358 Methylation
Y359 Phosphorylation
Y363 Phosphorylation
Y375 Phosphorylation
S381 Phosphorylation
R398 Methylation
K411 Ubiquitination
K413 Ubiquitination
K416 Ubiquitination
K418 Sumoylation
K418 Ubiquitination
Y430 Phosphorylation
R434 Methylation
T437 Phosphorylation
K448 Acetylation
C452 S-Nitrosylation
K455 Ubiquitination
K475 Acetylation
K475 Ubiquitination
T487 Phosphorylation
K493 Ubiquitination
K527 Ubiquitination
S530 Phosphorylation
S531 Phosphorylation
K533 Ubiquitination
S536 Phosphorylation
K538 Sumoylation
K538 Ubiquitination
S543 Phosphorylation
S544 Phosphorylation Q16566 (CAMK4)
K552 Acetylation
K552 Sumoylation
K552 Ubiquitination
S553 Phosphorylation
Y565 Phosphorylation
K568 Sumoylation
K568 Ubiquitination
Y574 Phosphorylation
Y576 Phosphorylation
T577 Phosphorylation
K579 Ubiquitination
C581 S-Nitrosylation
S589 Phosphorylation

研究背景

機能:

Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.

PTMs:

Several isoelectric forms of the L protein are probably the results of post-translational modifications.

Phosphorylation at Ser-544 by CaMK4 enhances interaction with a CaMK4-responsive RNA element (CaRRE1), and prevents inclusion of the stress axis-regulated exon (STREX) of the KCNMA1 potassium channel transcripts upon membrane depolarization.

細胞の位置付け:

Nucleus>Nucleoplasm. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. These granules are not identical with P bodies or stress granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with HNRNPLL. Interacts with APEX1; the interaction is DNA-dependent. Component of a complex with SETD2. Interacts with ELAVL1.

タンパク質ファミリー:

RRM domain 2 has moderate RNA-binding affinity. RRM domains 3 and 4 may facilitate RNA looping when binding to two appropriately separated binding sites within the same target pre-mRNA (PubMed:23782695).

参考文献

1). Stealthy nanoparticles protect endothelial barrier from leakiness by resisting the absorption of VE-cadherin. Nanoscale, 2021 (PubMed: 34259298) [IF=6.7]

Application: WB    Species: Human    Sample: HMVEC

Fig. 4 (a) Schematic showing the protein pull down experimental setup. Immunoblotting (left panel) and its semi-quantitative analysis (right panel) of (b) VEC, SOD1, claudin-5 and α-tublin from whole cell lysate and pulled down by different NPs. (c) Y658 (p-VEC(Y658)), Y731 (p-VEC(Y731), VEC and α-tublin from the whole cell lysate with/without the Src kinase inhibitor, PP1.

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