hnRNP M Antibody - #AF5276
(2)
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
hnRNP M Antibody detects endogenous levels of total hnRNP M.
RRID:
AB_2837762
引用形式: Affinity Biosciences Cat# AF5276, RRID:AB_2837762.
引用形式: Affinity Biosciences Cat# AF5276, RRID:AB_2837762.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
CEA receptor; CEAR; Heterogeneous nuclear ribonucleoprotein M; Heterogenous nuclear ribonucleoprotein M4; hnRNA binding protein M4; hnRNP M; Hnrnpm; HNRNPM4; HNRPM; HNRPM_HUMAN; HNRPM4; HTGR1; M3; M4; M4 protein; N-acetylglucosamine receptor 1; NAGR1;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
タンパク質配列:
- P52272 HNRPM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMITIPPSILNNPNIPNEIIHALQAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHLNKGIGMGNIGPAGMGMEGIGFGINKMGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGIERMGPGIDRLGGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMGSGIERMGPLGLDHMASSIERMGQTMERIGSGVERMGAGMGFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMERMVPAGMGAGLERMGPVMDRMATGLERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALGAGIERMGLAMGGGGGASFDRAIEMERGNFGGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDRNA
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
73
Xenopus
70
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P52272 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| K17 | Sumoylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K37 | Sumoylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| K52 | Methylation | Uniprot | |
| K55 | Sumoylation | Uniprot | |
| R56 | Methylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| T76 | Phosphorylation | Uniprot | |
| K83 | Acetylation | Uniprot | |
| K83 | Sumoylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| K88 | Sumoylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| Y100 | Phosphorylation | Uniprot | |
| K110 | Acetylation | Uniprot | |
| K110 | Sumoylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| C114 | S-Nitrosylation | Uniprot | |
| K120 | Sumoylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K126 | Sumoylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K127 | Sumoylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K134 | Acetylation | Uniprot | |
| K134 | Sumoylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| K143 | Sumoylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K145 | Sumoylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| T205 | Phosphorylation | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| K214 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K221 | Sumoylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K239 | Acetylation | Uniprot | |
| K239 | Sumoylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K242 | Sumoylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K277 | Acetylation | Uniprot | |
| K277 | Sumoylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K322 | Sumoylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| R371 | Methylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| K381 | Acetylation | Uniprot | |
| K381 | Sumoylation | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| K388 | Sumoylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| S397 | Phosphorylation | Uniprot | |
| R410 | Methylation | Uniprot | |
| R429 | Methylation | Uniprot | |
| S432 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| R443 | Methylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| S468 | Phosphorylation | Uniprot | |
| T475 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot | |
| R485 | Methylation | Uniprot | |
| R496 | Methylation | Uniprot | |
| R503 | Methylation | Uniprot | |
| T507 | Phosphorylation | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| R543 | Methylation | Uniprot | |
| R550 | Methylation | Uniprot | |
| R565 | Methylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| R621 | Methylation | Uniprot | |
| R627 | Methylation | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| S637 | Phosphorylation | Uniprot | |
| K651 | Sumoylation | Uniprot | |
| K651 | Ubiquitination | Uniprot | |
| T665 | Phosphorylation | Uniprot | |
| K667 | Acetylation | Uniprot | |
| K667 | Sumoylation | Uniprot | |
| K667 | Ubiquitination | Uniprot | |
| K670 | Ubiquitination | Uniprot | |
| K672 | Acetylation | Uniprot | |
| K672 | Sumoylation | Uniprot | |
| K672 | Ubiquitination | Uniprot | |
| C676 | S-Nitrosylation | Uniprot | |
| Y681 | Phosphorylation | Uniprot | |
| K685 | Acetylation | Uniprot | |
| K685 | Sumoylation | Uniprot | |
| K685 | Ubiquitination | Uniprot | |
| K690 | Ubiquitination | Uniprot | |
| K692 | Ubiquitination | Uniprot | |
| C694 | S-Nitrosylation | Uniprot | |
| K698 | Acetylation | Uniprot | |
| K698 | Sumoylation | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| S701 | Phosphorylation | Uniprot | |
| C709 | S-Nitrosylation | Uniprot | |
| K716 | Sumoylation | Uniprot | |
| K716 | Ubiquitination | Uniprot |
研究背景
機能:
Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
PTMs:
Sumoylated.
細胞の位置付け:
Nucleus>Nucleolus.
サブユニット構造:
Identified in the spliceosome C complex.
研究領域
· Genetic Information Processing > Transcription > Spliceosome.
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