ZEB2 Antibody - #AF5278
(6)
(7)
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Pig(91%), Bovine(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
ZEB2 Antibody detects endogenous levels of total ZEB2.
RRID:
AB_2837764
引用形式: Affinity Biosciences Cat# AF5278, RRID:AB_2837764.
引用形式: Affinity Biosciences Cat# AF5278, RRID:AB_2837764.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
FLJ42816; HSPC082; KIAA0569; SIP 1; SIP1; Smad-interacting protein 1; SMADIP 1; SMADIP1; ZEB 2; Zeb2; ZEB2_HUMAN; Zfhx1b; ZFHX1B protein; Zfx1b; Zinc finger E box binding protein 2; Zinc finger E-box-binding homeobox 2; Zinc finger homeobox 1b; zinc finger homeobox protein 1; Zinc finger homeobox protein 1b;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
タンパク質配列:
- O60315 ZEB2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKQPIMADGPRCKRRKQANPRRKNVVNYDNVVDTGSETDEEDKLHIAEDDGIANPLDQETSPASVPNHESSPHVSQALLPREEEEDEIREGGVEHPWHNNEILQASVDGPEEMKEDYDTMGPEATIQTAINNGTVKNANCTSDFEEYFAKRKLEERDGHAVSIEEYLQRSDTAIIYPEAPEELSRLGTPEANGQEENDLPPGTPDAFAQLLTCPYCDRGYKRLTSLKEHIKYRHEKNEENFSCPLCSYTFAYRTQLERHMVTHKPGTDQHQMLTQGAGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLISVNGRMRNNIKTGSSPNSVSSSPTNSAITQLRNKLENGKPLSMSEQTGLLKIKTEPLDFNDYKVLMATHGFSGTSPFMNGGLGATSPLGVHPSAQSPMQHLGVGMEAPLLGFPTMNSNLSEVQKVLQIVDNTVSRQKMDCKAEEISKLKGYHMKDPCSQPEEQGVTSPNIPPVGLPVVSHNGATKSIIDYTLEKVNEAKACLQSLTTDSRRQISNIKKEKLRTLIDLVTDDKMIENHNISTPFSCQFCKESFPGPIPLHQHERYLCKMNEEIKAVLQPHENIVPNKAGVFVDNKALLLSSVLSEKGMTSPINPYKDHMSVLKAYYAMNMEPNSDELLKISIAVGLPQEFVKEWFEQRKVYQYSNSRSPSLERSSKPLAPNSNPPTKDSLLPRSPVKPMDSITSPSIAELHNSVTNCDPPLRLTKPSHFTNIKPVEKLDHSRSNTPSPLNLSSTSSKNSHSSSYTPNSFSSEELQAEPLDLSLPKQMKEPKSIIATKNKTKASSISLDHNSVSSSSENSDEPLNLTFIKKEFSNSNNLDNKSTNPVFSMNPFSAKPLYTALPPQSAFPPATFMPPVQTSIPGLRPYPGLDQMSFLPHMAYTYPTGAATFADMQQRRKYQRKQGFQGELLDGAQDYMSGLDDMTDSDSCLSRKKIKKTESGMYACDLCDKTFQKSSSLLRHKYEHTGKRPHQCQICKKAFKHKHHLIEHSRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEEREAAEREAREKGHLEPTELLMNRAYLQSITPQGYSDSEERESMPRDGESEKEHEKEGEDGYGKLGRQDGDEEFEEEEEESENKSMDTDPETIRDEEETGDHSMDDSSEDGKMETKSDHEEDNMEDGM
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Sheep
100
Dog
100
Rabbit
100
Pig
91
Bovine
91
Xenopus
64
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60315 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R11 | Methylation | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| Y147 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| T285 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| T350 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| T362 | Phosphorylation | Uniprot | |
| K377 | Acetylation | Uniprot | |
| K391 | Sumoylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| T470 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| K632 | Ubiquitination | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| S647 | Phosphorylation | Uniprot | |
| S703 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| S707 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| S731 | Phosphorylation | Uniprot | |
| S741 | Phosphorylation | Uniprot | |
| S778 | Phosphorylation | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| T782 | Phosphorylation | Uniprot | |
| S784 | Phosphorylation | Uniprot | |
| S796 | Phosphorylation | Uniprot | |
| T802 | Phosphorylation | Uniprot | |
| S805 | Phosphorylation | Uniprot | |
| S819 | Phosphorylation | Uniprot | |
| S829 | Phosphorylation | Uniprot | |
| T837 | Phosphorylation | Uniprot | |
| S840 | Phosphorylation | Uniprot | |
| S841 | Phosphorylation | Uniprot | |
| S843 | Phosphorylation | Uniprot | |
| S848 | Phosphorylation | Uniprot | |
| S853 | Phosphorylation | Uniprot | |
| K866 | Sumoylation | Uniprot | |
| S1050 | Phosphorylation | Uniprot | |
| S1115 | Phosphorylation | Uniprot | |
| T1117 | Phosphorylation | Uniprot | |
| S1122 | Phosphorylation | Uniprot | |
| S1124 | Phosphorylation | Uniprot | |
| S1129 | Phosphorylation | Uniprot | |
| S1136 | Phosphorylation | Uniprot | |
| S1167 | Phosphorylation | Uniprot | |
| T1185 | Phosphorylation | Uniprot | |
| S1189 | Phosphorylation | Uniprot | |
| S1193 | Phosphorylation | Uniprot | |
| S1194 | Phosphorylation | Uniprot | |
| S1203 | Phosphorylation | Uniprot |
研究背景
機能:
Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
PTMs:
Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.
細胞の位置付け:
Nucleus.
サブユニット構造:
Binds activated SMAD1, activated SMAD2 and activated SMAD3; binding with SMAD4 is not detected (By similarity). Interacts with CBX4 and CTBP1.
タンパク質ファミリー:
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
研究領域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
参考文献
1). Fisetin Improves Hyperuricemia-Induced Chronic Kidney Disease via Regulating Gut Microbiota-Mediated Tryptophan Metabolism and Aryl Hydrocarbon Receptor Activation. Journal of Agricultural and Food Chemistry, 2021
[IF=6.1]
2). The regulatory effects of metformin on the [SNAIL/miR-34]:[ZEB/miR-200] system in the epithelial-mesenchymal transition(EMT) for colorectal cancer(CRC). European Journal of Pharmacology, 2018
(PubMed: 30017802)
[IF=5.0]
Application: WB Species: human Sample: SW480 and HCT116 cells
Fig. 3. |Western-blot for SW480 and HCT116 cells (A)(B) Protein strips and quantitative analysis: significant decreases in the SNAIL1, ZEB1 and vimentin and a significant increase in the E-cadherin of metformin and TGF-β-treated samples compared with only TGF-β-treated samples for both SW480 and HCT116 cells; a significant increase in the ZEB2 of metformin and TGF-β-treated samples compared with only TGF-β-treated samples for SW480 cells; significant decreases in the SNAIL2, ZEB1 and ZEB2 of metformin-treated samples compared with negative control samples for HCT116 cells. *P<0.05
3). Network pharmacology- and cell-based assessments identify the FAK/Src pathway as a molecular target for the antimetastatic effect of momordin Ic against cholangiocarcinoma. Heliyon, 2024
(PubMed: 38961933)
[IF=4.0]
Application: WB Species: Human Sample: KKU-452 cells
Fig. 5 Effects of MIc on EMT in CCA cells. KKU-452 cells were chemically induced to undergo EMT (iEMT). (A) The effect of MIc on cell morphology at concentrations of 1 and 2.5 μM was determined. EMT marker expression, including N-cadherin, vimentin, ZEB2, and FOXC1/2, was further evaluated by (B) Western blot (Supplementary Fig. S1 Images of original blot 5B) and (C) immunocytofluorescence analyses. The data shown are representative of two reproducible experiments.
4). Estradiol promotes EMT in endometriosis via MALAT1/miR200s sponge function. REPRODUCTION, 2019
(PubMed: 30500775)
[IF=3.8]
Application: WB Species: human Sample: EECs
Figure 2.| (A and B) Expression levels of EMT-associated markers (Zeb1, Zeb2, E-cadherin and Vimentin) with E2 treatment for different doses (0, 10−12, 10−10, 10−8, 10−6 mol/L) and time (0, 24, 48 or 72 h) in EECs detected by Western blot.(M: mol/L); (C and D) Expression levels of MALAT1 and miR200s under E2 for various doses (0, 10−12, 10−10, 10−8, 10−6 mol/L) for 24 h in EECs measured by qRT-PCR. Data were evaluated by one-way ANOVA analysis (*P < 0.05, **P < 0.01, ***P < 0.001 compared with untreated group).
5). Exosomal miRNA-215-5p Derived from Adipose-Derived Stem Cells Attenuates Epithelial–Mesenchymal Transition of Podocytes by Inhibiting ZEB2. BioMed Research International, 2020
(PubMed: 32149094)
Application: WB Species: mouse Sample: MPC5 cells
Figure 5: |The correlation between miR-215-5p and ZEB2 in podocyte migration. (a) Targeted binding sites between miR-215-5p and ZEB2-3′-UTR sequences were predicted by TargetScan database and effect of miR-215-5p on transcription activity of ZEB2 determined by luciferase report assay in 293 cells. (b) The expression of ZEB2 was measured by qRT-PCR in 293 cells treated with miR-215-5p mimic.(c) The protein level of ZEB2 was measured by western blotting in MPC5 cells treated with miR-215-5p mimic
6). Wnt/PCP-YAP-BIRC2 axis maintains cartilage stem/progenitor cell homeostasis in osteoarthritis. Research Square, 2022
Application: WB Species: Human Sample: CSPCs
Figure 7. Transcriptomic analysis. a KEGG and GO enrichment analysis of DEGs. b The detailed DEGs that enriched in cell cycle, cellular senescence, autophagy, apoptosis, Hippo signaling pathway, and TNF signaling pathways. c The direct downstream target genes of YAP. d The protein level of Birc2, Snai2, Zenb2, and Ccnd2 in CSPCs.
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