PSD95 Antibody - #AF5283

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製品: PSD95 Antibody
カタログ: AF5283
タンパク質の説明: Rabbit polyclonal antibody to PSD95
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Zebrafish, Bovine, Horse, Sheep, Rabbit
分子量: 105 kDa; 80kD(Calculated).
ユニプロット: P78352
RRID: AB_2827690

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
PSD95 Antibody detects endogenous levels of total PSD95.
RRID:
AB_2827690
引用形式: Affinity Biosciences Cat# AF5283, RRID:AB_2827690.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Discs large homolog 4; Disks large homolog 4; DLG 4; Dlg4; DLG4_HUMAN; FLJ97752; FLJ98574; Human post synaptic density protein 95; Post synaptic density protein 95; Postsynaptic density protein 95; PSD 95; PSD-95; PSD95; SAP 90; SAP-90; SAP90; Synapse associated protein 90; Synapse-associated protein 90; Tax interaction protein 15;

免疫原

免疫原:
Uniprot:

P78352(DLG4_HUMAN) >>Visit HPA database.

遺伝子(ID):
DLG4(1742)
発現特異性:
P78352 DLG4_HUMAN:

Brain.

タンパク質の説明:
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons.
タンパク質配列:
MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Zebrafish
80
Pig
0
Dog
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78352 基板として

Site PTM Type Enzyme
T8 Phosphorylation
T19 Phosphorylation
S73 Phosphorylation
S142 Phosphorylation
Y147 Phosphorylation
T192 Phosphorylation
Y236 Phosphorylation
Y240 Phosphorylation
T287 Phosphorylation P53778 (MAPK12) , O15264 (MAPK13) , P28482 (MAPK1)
S290 Phosphorylation P53778 (MAPK12)
S295 Phosphorylation P45983 (MAPK8)
Y397 Phosphorylation
K403 Ubiquitination
S425 Phosphorylation
Y523 Phosphorylation P06241 (FYN) , P12931 (SRC)
S561 Phosphorylation Q7KZI7 (MARK2)
Y573 Phosphorylation
Y609 Phosphorylation
Y701 Phosphorylation

研究背景

機能:

Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression.

PTMs:

Palmitoylated. Palmitoylation is required for targeting to postsynaptic density, plasma membrane and synapses (By similarity). Palmitoylation may play a role in glutamate receptor GRIA1 synapse clustering (By similarity). Depalmitoylated by ABHD17A and ABHD17B and to a lesser extent by ABHD17C, ABHD12, ABHD13, LYPLA1 and LYPLA2. Undergoes rapid synaptic palmitoylation/depalmitoylation cycles during neuronal development which slow down in mature neurons (By similarity).

Ubiquitinated by MDM2 in response to NMDA receptor activation, leading to proteasome-mediated degradation of DLG4 which is required for AMPA receptor endocytosis.

細胞の位置付け:

Cell membrane>Lipid-anchor>Cytoplasmic side. Cell junction>Synapse>Postsynaptic density. Cell junction>Synapse. Cytoplasm. Cell projection>Axon. Cell projection>Dendritic spine. Cell projection>Dendrite. Cell junction>Synapse>Presynapse.
Note: High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Brain.

サブユニット構造:

Interacts through its PDZ domains with ANO2 and NETO1 (By similarity). Interacts through its first two PDZ domains with GRIN2A, GRIN2B, GRIN2C, GRIN2D (By similarity). Interacts with ASIC3 (By similarity). Interacts with SEMA4C (By similarity). Interacts with CXADR (By similarity). Interacts with KCND2 (By similarity). Interacts with SYNGAP1 (By similarity). Interacts with LRRC4 and LRRC4B (By similarity). Interacts with ERBB4. Interacts with KCNA1, KCNA2, KCNA3 and KCNA4. Interacts through its first PDZ domain with GRIK2, KCNA4 and CRIPT. Interacts through its second PDZ domain with the PDZ domain of NOS1 or the C-terminus of CAPON (By similarity). Interacts through its third PDZ domain with NLGN1 and CRIPT, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with KIF13B. Interacts through its guanylate kinase-like domain with DLGAP1/GKAP, DLGAP2, DLGAP3, DLGAP4, MAP1A, BEGAIN and SIPA1L1 (By similarity). Isoform 2 interacts through an L27 domain with HGS/HRS and the first L27 domain of CASK. Interacts with ADR1B and ANKS1B (By similarity). May interact with HTR2A (By similarity). Interacts with ADAM22. Interacts with KLHL17 and LGI1 (By similarity). Interacts with FRMPD4 (via C-terminus). Interacts with LRFN1, LRFN2 and LRFN4. Interacts (via N-terminal tandem pair of PDZ domains) with GPER1 (via C-terminus tail motif); the interaction is direct and induces the increase of GPER1 protein levels residing at the plasma membrane surface in a estradiol-independent manner (By similarity). Interacts (via N-terminus tandem pair of PDZ domains) with NOS1 (via N-terminal domain) (By similarity). Interacts with SHANK3 (By similarity). Interacts with KCNJ4 (By similarity). Interacts with GPR85. Interacts with CACNG2 and MPP2 (via the SH3-Guanylate kinase-like sub-module) (By similarity). Interacts with ADGRB1. Found in a complex with PRR7 and GRIN1 (By similarity). Interacts (via PDZ3 domain and to lesser degree via PDZ2 domain) with PRR7 (By similarity). Component of the postsynaptic hippocampal AMPA-type glutamate receptor (AMPAR) complex, at least composed of pore forming AMPAR subunits GRIA1, GRIA2 and GRIA3 and AMPAR auxiliary proteins SHISA6 and SHISA7. Interacts (via its first two PDZ domains) with SHISA6 and SHISA7 (via PDZ-binding motif); the interaction is direct (By similarity). Interacts with RPH3A and GRIN2A; this ternary complex regulates NMDA receptor composition at postsynaptic membranes (By similarity). Interacts with ABR and BCR.

タンパク質ファミリー:

The PDZ domain 3 mediates interaction with ADR1B.

The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.

Belongs to the MAGUK family.

研究領域

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Organismal Systems > Nervous system > Glutamatergic synapse.

参考文献

1). SiNiSan ameliorates depression-like behavior in rats by enhancing synaptic plasticity via the CaSR-PKC-ERK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2020 (PubMed: 31958763) [IF=7.5]

Application: WB    Species: rat    Sample: HIP

Fig. 5. |Effects of SNS on synaptic-associated protein in the HIP and PFC of stressed rats. Representative immunoblots for PSD-95, GAP-43, Syn, and Tublin in the HIP(A) and PFC(B) regions. (A) Results of relative protein levels of PSD-95, GAP-43, and Syn in the HIP of rats of each group.
2). Cyfluthrin exposure during pregnancy causes neurotoxicity in offspring-Ca2+ overload via IP3R-GRP75-VDAC1 pathway. Ecotoxicology and environmental safety, 2024 (PubMed: 38492481) [IF=6.8]
3). Exosomes derived from human induced pluripotent stem cell-derived neural progenitor cells protect neuronal function under ischemic conditions. Neural Regeneration Research, 2021 (PubMed: 33642395) [IF=6.1]

Application: WB    Species: Rat    Sample: neural progenitor cells

Figure 3 Effect of iPSC-NPC-derived exosomes (OGD+iNPC-exo) on expression of the PTEN/AKT signaling pathway and of synaptic plasticity-related proteins in OGD induced neurons. (A–C) mRNA expression (A) and protein expression (B, C) of the PTEN/AKT signaling pathway and of synaptic plasticity-related proteins (NF200, GAP-43, Synapsin, and PSD95) analyzed by polymerase chain reaction and western blot assay. The mRNA expression is described by the optical density ratio relative to the control group. Protein expression was described by the optical density ratio relative to β-actin. Data are presented as mean ± SD. ***P < 0.001, vs. control group; ###P < 0.001 (one-way analysis of variance with post hoc Bonferroni test). All experiments were repeated three times. GAP43: growth associated protein 43; iPSC-NPCs: induced pluripotent stem cells-derived neural progenitor cells; NF200: neurofilament 200; OGD: oxygen-glucose deprivation; p-Akt: phosphor-Akt; PSD95: postsynaptic density protein 95; PTEN: phosphatase and tensin homolog deleted on chromosome ten.
4). Early-Life Stress Induces Depression-Like Behavior and Synaptic-Plasticity Changes in a Maternal Separation Rat Model: Gender Difference and Metabolomics Study. Frontiers in Pharmacology, 2020 (PubMed: 32174832) [IF=5.6]

Application: WB    Species: Rat    Sample: hippocampus

Figure 5 MS reduces the expression of synaptic-plasticity protein. (A) The bands of synaptic-plasticity proteins of SYN, PSD-95, and GAP-43 in the hippocampus by WB. Statistical results indicate the relative protein levels expressed by SYN, GAP-43, and PSD-95. (B) The bands of synaptic-plasticity proteins of SYN, PSD-95, and GAP-43 in cortex by WB. Statistical results indicate the relative protein levels expressed by SYN, GAP-43, and PSD-95. Statistical analyses are performed by two-way ANOVA followed by t-test. Data are presented as mean ± SEM, *p < 0.05, **p < 0.01, n = 3 per group.
5). Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn. NEUROPHARMACOLOGY, 2019 (PubMed: 30721695) [IF=4.7]
6). The Effect of Early Maternal Separation Combined With Adolescent Chronic Unpredictable Mild Stress on Behavior and Synaptic Plasticity in Adult Female Rats. Frontiers in Psychiatry, 2021 (PubMed: 33746787) [IF=4.7]

Application: WB    Species: Rat    Sample: Hippocampus

Figure 5 Western blot analysis. The expressions of synaptic plasticity proteins were determined by western blot (A), including PSD-95 (B), GAP-43 (C), and SYN (D). The values represent the mean ± SEM, n = 5. *p < 0.05, **p < 0.01 vs. CON, #p < 0.05, ##p < 0.01 vs. MS. CON, control; MS, maternal separation; CUMS, chronic unpredictable mild stress.
7). Adverse effects of iron deficiency anemia on pregnancy outcome and offspring development and intervention of three iron supplements. Scientific Reports, 2021 (PubMed: 33446747) [IF=4.6]

Application: WB    Species: Rat    Sample: hippocampus

Figure 5 Iron related indexes and neural development of offspring rats after iron supplement treatment. Western blot analysis for FTL and Tf in liver (a–c) and spleen (d–f). Morris water maze test for day 1 escape latency (g) and day 2 escape latency (h). Western blot analysis for FTL and Tf in brain (i–k) and hippocampus (l–n), and for SYN1, NMDAR, PSD-95 in hippocampus (o–r). The quantification of western blotting was provided in supplementary material. FTL ferritin light chain, Tf transferrin, SYN1 synapsin 1, NMDAR N-methyl-D-aspartate receptor, PSD-95 postsynaptic density protein 95. Data of Western blot analysis (mean ± SD) are expressed as the ratio of the relative contents between the value from IDA group and NG group and six iron treatment groups (n = 3). The relative contents of target proteins were quantified using the ratio between the optical density (OD) of target protein and the amount of the housekeeping protein GAPDH. **p < 0.01, compared with NG, #p < 0.05, ##p < 0.01, compared with IDAG. One-way ANOVA followed by Tukey multiple comparison test was used for comparison among 8 different groups.
8). CHIP Decline Is Associated With Isoflurane-Induced Neurodegeneration in Aged Mice. Frontiers in Neuroscience, 2022 (PubMed: 35368262) [IF=4.3]

Application: WB    Species: mouse    Sample: N2a cells

FIGURE 6 | Decreased CHIP expression altered synaptic protein expression and phosphorylation in N2a cells. (A) CHIP knock-down protein was designed by siRNA in N2a cells. Approximately 5 µl Stub1-siRNA was able to achieve effective knock-down CHIP expression, and consequently the expression level of synapsin I S9 was decreased with no change in synaptic protein SNAP25 and PSD95 expression.
9). Regular Exercise Enhances Cognitive Function and Intracephalic GLUT Expression in Alzheimer\'s Disease Model Mice. JOURNAL OF ALZHEIMERS DISEASE, 2019 (PubMed: 31561359) [IF=4.0]

Application: WB    Species: mouse    Sample: hippocampus

Fig. 4. The expression of PSD-95, SYN, and BDNF. Representative immunoblots of PSD-95, SYN, and BDNF in the cortex (A-D) and hippocampus (E-H) in each group. Protein immunoreactivity was normalized to -actin. Individual data are presented as the mean ± S.E.M. from 4 individual mice in each group.
10). Increased Fibronectin Impairs the Function of Excitatory/Inhibitory Synapses in Hirschsprung Disease. CELLULAR AND MOLECULAR NEUROBIOLOGY, 2020 (PubMed: 31760535) [IF=4.0]

Application: IF/ICC    Species: rat    Sample: PC12 cell

Fig. 3|Suppression of FN increases the expression of synaptic proteins. A Relative expression of NL-1, PSD-95, vGLUT1, NL-2, SLC32, and FN mRNAs were signifcantly increased in the FN-KD group compared with the NC group and B Western blots of PC12 cell lysates in the FN-KD, NC, and Ctr groups with NL-1, PSD-95, NL-2, SLC32, and FN antibodies. The quantitative results (right) confrm that silencing FN signifcantly increases the expression of neuronal marker proteins.C The co-expression of NL-1, or NL-2, or PSD-95, or SLC32 with FN was examined by double immunofuorescence staining. The results support the idea that downregulation of FN enhances NL-1, PSD-95, NL-2, and SLC32 expression. Values are mean ± SEM, *P < 0.05, **P < 0.01, and ***P < 0.001

Application: IHC    Species: human    Sample: ganglionic, transitional, and aganglionic colonic segments

Fig. 2|Quantitative histological evaluations of ganglionic, transitional, and aganglionic colonic segments of HSCR patients. A1–3, a1–3 PSD-95 group, B1–3, b1–3 SLC32 group, C1–3, c1–3 FN group, d histological score, based on the intensity of positive staining (brown), and e area of positive staining shown as a percentage of total tissue area. Figures are representative of at least fve separate experiments. The diferences among the three groups were all statistically signifcant. Values are mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (ganglionic vs. aganglionic, P < 0.0001)
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